Assessment of γh2AX nuclear foci number and size in normal and repair-deficient cells irradiated with low and high linear energy transfer radiation

The aim of this study was to evaluate the DNA damage induced by low and high linear energy transfer radiation in two related cell lines with different radiation sensitivity, CHO10B2 and irs-20 cells (defective in DNAPKcs). Double-strand breaks were assessed by the detection of phosphorylated histone...

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Autores principales:	Molinari de Rey, Beatriz, Palmieri, Mónica Alejandra, Maglioco, Andrea Florencia, Policastro, Lucía Laura, Kreiner, Andrés Juan, Burlon, Alejandro, Davidson, Jorge, Davidson, Miguel, Durán, Hebe Alicia
Publicado:	2010
Materias:	γH2AX CHO10B2 DNA double strand break DNA-PKcs H2AX foci size Ionising radiation irs-20 LET Linear energy transfer histone gamma h2ax histone H2AX lithium unclassified drug animal cell article controlled study DNA damage double stranded DNA break gamma irradiation immunofluorescence ionizing radiation linear energy transfer nonhuman protein phosphorylation radiation dose fractionation radiosensitivity
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14776545_v7_n5_p393_Bracalente http://hdl.handle.net/20.500.12110/paper_14776545_v7_n5_p393_Bracalente
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	paper:paper_14776545_v7_n5_p393_Bracalente2023-06-08T16:18:10Z Assessment of γh2AX nuclear foci number and size in normal and repair-deficient cells irradiated with low and high linear energy transfer radiation Molinari de Rey, Beatriz Palmieri, Mónica Alejandra Maglioco, Andrea Florencia Policastro, Lucía Laura Kreiner, Andrés Juan Burlon, Alejandro Davidson, Jorge Davidson, Miguel Durán, Hebe Alicia γH2AX CHO10B2 DNA double strand break DNA-PKcs H2AX foci size Ionising radiation irs-20 LET Linear energy transfer histone gamma h2ax histone H2AX lithium unclassified drug animal cell article controlled study DNA damage double stranded DNA break gamma irradiation immunofluorescence ionizing radiation linear energy transfer nonhuman protein phosphorylation radiation dose fractionation radiosensitivity The aim of this study was to evaluate the DNA damage induced by low and high linear energy transfer radiation in two related cell lines with different radiation sensitivity, CHO10B2 and irs-20 cells (defective in DNAPKcs). Double-strand breaks were assessed by the detection of phosphorylated histone H2AX foci (γH2AX). The number of foci increased as a function of dose for both cell lines and types of radiation. However, irs-20 cells showed a higher number of foci than the parental CHO10B2. An increase in the foci size was observed for both cell lines after lithium irradiation. This could be attributed to clusters of DNA damage. Moreover, the number of lithiuminduced larger foci per nuclei increased with dose and fitted with the expected number of hits per nucleus. Concluding, γH2AX foci size provides a potential tool to characterise the intrinsic radiosensitivity of cells related to DNA damage and to compare the effects of different quality radiations. Copyright © 2010 Inderscience Enterprises Ltd. Fil:Molinari, B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Palmieri, M.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Maglioco, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Policastro, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Kreiner, A.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Burlón, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Davidson, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Davidson, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Durán, H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2010 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14776545_v7_n5_p393_Bracalente http://hdl.handle.net/20.500.12110/paper_14776545_v7_n5_p393_Bracalente
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	γH2AX CHO10B2 DNA double strand break DNA-PKcs H2AX foci size Ionising radiation irs-20 LET Linear energy transfer histone gamma h2ax histone H2AX lithium unclassified drug animal cell article controlled study DNA damage double stranded DNA break gamma irradiation immunofluorescence ionizing radiation linear energy transfer nonhuman protein phosphorylation radiation dose fractionation radiosensitivity
spellingShingle	γH2AX CHO10B2 DNA double strand break DNA-PKcs H2AX foci size Ionising radiation irs-20 LET Linear energy transfer histone gamma h2ax histone H2AX lithium unclassified drug animal cell article controlled study DNA damage double stranded DNA break gamma irradiation immunofluorescence ionizing radiation linear energy transfer nonhuman protein phosphorylation radiation dose fractionation radiosensitivity Molinari de Rey, Beatriz Palmieri, Mónica Alejandra Maglioco, Andrea Florencia Policastro, Lucía Laura Kreiner, Andrés Juan Burlon, Alejandro Davidson, Jorge Davidson, Miguel Durán, Hebe Alicia Assessment of γh2AX nuclear foci number and size in normal and repair-deficient cells irradiated with low and high linear energy transfer radiation
topic_facet	γH2AX CHO10B2 DNA double strand break DNA-PKcs H2AX foci size Ionising radiation irs-20 LET Linear energy transfer histone gamma h2ax histone H2AX lithium unclassified drug animal cell article controlled study DNA damage double stranded DNA break gamma irradiation immunofluorescence ionizing radiation linear energy transfer nonhuman protein phosphorylation radiation dose fractionation radiosensitivity
description	The aim of this study was to evaluate the DNA damage induced by low and high linear energy transfer radiation in two related cell lines with different radiation sensitivity, CHO10B2 and irs-20 cells (defective in DNAPKcs). Double-strand breaks were assessed by the detection of phosphorylated histone H2AX foci (γH2AX). The number of foci increased as a function of dose for both cell lines and types of radiation. However, irs-20 cells showed a higher number of foci than the parental CHO10B2. An increase in the foci size was observed for both cell lines after lithium irradiation. This could be attributed to clusters of DNA damage. Moreover, the number of lithiuminduced larger foci per nuclei increased with dose and fitted with the expected number of hits per nucleus. Concluding, γH2AX foci size provides a potential tool to characterise the intrinsic radiosensitivity of cells related to DNA damage and to compare the effects of different quality radiations. Copyright © 2010 Inderscience Enterprises Ltd.
author	Molinari de Rey, Beatriz Palmieri, Mónica Alejandra Maglioco, Andrea Florencia Policastro, Lucía Laura Kreiner, Andrés Juan Burlon, Alejandro Davidson, Jorge Davidson, Miguel Durán, Hebe Alicia
author_facet	Molinari de Rey, Beatriz Palmieri, Mónica Alejandra Maglioco, Andrea Florencia Policastro, Lucía Laura Kreiner, Andrés Juan Burlon, Alejandro Davidson, Jorge Davidson, Miguel Durán, Hebe Alicia
author_sort	Molinari de Rey, Beatriz
title	Assessment of γh2AX nuclear foci number and size in normal and repair-deficient cells irradiated with low and high linear energy transfer radiation
title_short	Assessment of γh2AX nuclear foci number and size in normal and repair-deficient cells irradiated with low and high linear energy transfer radiation
title_full	Assessment of γh2AX nuclear foci number and size in normal and repair-deficient cells irradiated with low and high linear energy transfer radiation
title_fullStr	Assessment of γh2AX nuclear foci number and size in normal and repair-deficient cells irradiated with low and high linear energy transfer radiation
title_full_unstemmed	Assessment of γh2AX nuclear foci number and size in normal and repair-deficient cells irradiated with low and high linear energy transfer radiation
title_sort	assessment of γh2ax nuclear foci number and size in normal and repair-deficient cells irradiated with low and high linear energy transfer radiation
publishDate	2010
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14776545_v7_n5_p393_Bracalente http://hdl.handle.net/20.500.12110/paper_14776545_v7_n5_p393_Bracalente
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Assessment of γh2AX nuclear foci number and size in normal and repair-deficient cells irradiated with low and high linear energy transfer radiation

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