Virus infection elevates transcriptional activity of miR164a promoter in plants

Background. Micro RNAs (miRs) constitute a large group of endogenous small RNAs that have crucial roles in many important plant functions. Virus infection and transgenic expression of viral proteins alter accumulation and activity of miRs and so far, most of the published evidence involves post-tran...

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Autores principales:	Bazzini, Ariel Alejandro, Almasia, Natalia Inés, Mongelli, Vanesa Claudia, Maroniche, Guillermo Andrés, Rodriguez, María Cecilia, Distéfano, Ana Julia, Hopp, Horacio Esteban, Del Vas, Mariana, Asurmendi, Sebastián
Publicado:	2009
Materias:	Arabidopsis Brassica napus Chinese Rape Mosaic Virus Tobacco mosaic virus microRNA microRNA 164, Arabidopsis microRNA-164, Arabidopsis phytohormone plant RNA article biology gene expression regulation genetics metabolism molecular cloning Mosaic virus physiology plant disease promoter region reporter gene transgenic plant virology Cloning, Molecular Computational Biology Gene Expression Regulation, Developmental Gene Expression Regulation, Plant Genes, Reporter MicroRNAs Mosaic Viruses Plant Diseases Plant Growth Regulators Plants, Genetically Modified Promoter Regions, Genetic RNA, Plant
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14712229_v9_n_p_Bazzini http://hdl.handle.net/20.500.12110/paper_14712229_v9_n_p_Bazzini
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_14712229_v9_n_p_Bazzini
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spelling	paper:paper_14712229_v9_n_p_Bazzini2023-06-08T16:17:19Z Virus infection elevates transcriptional activity of miR164a promoter in plants Bazzini, Ariel Alejandro Almasia, Natalia Inés Mongelli, Vanesa Claudia Maroniche, Guillermo Andrés Rodriguez, María Cecilia Distéfano, Ana Julia Hopp, Horacio Esteban Del Vas, Mariana Asurmendi, Sebastián Arabidopsis Brassica napus Chinese Rape Mosaic Virus Tobacco mosaic virus microRNA microRNA 164, Arabidopsis microRNA-164, Arabidopsis phytohormone plant RNA Arabidopsis article biology gene expression regulation genetics metabolism molecular cloning Mosaic virus physiology plant disease promoter region reporter gene transgenic plant virology Arabidopsis Cloning, Molecular Computational Biology Gene Expression Regulation, Developmental Gene Expression Regulation, Plant Genes, Reporter MicroRNAs Mosaic Viruses Plant Diseases Plant Growth Regulators Plants, Genetically Modified Promoter Regions, Genetic RNA, Plant Background. Micro RNAs (miRs) constitute a large group of endogenous small RNAs that have crucial roles in many important plant functions. Virus infection and transgenic expression of viral proteins alter accumulation and activity of miRs and so far, most of the published evidence involves post-transcriptional regulations. Results. Using transgenic plants expressing a reporter gene under the promoter region of a characterized miR (P-miR164a), we monitored the reporter gene expression in different tissues and during Arabidopsis development. Strong expression was detected in both vascular tissues and hydathodes. P-miR164a activity was developmentally regulated in plants with a maximum expression at stages 1.12 to 5.1 (according to Boyes, 2001) along the transition from vegetative to reproductive growth. Upon quantification of P-miR164a-derived GUS activity after Tobacco mosaic virus Cg or Oilseed rape mosaic virus (ORMV) infection and after hormone treatments, we demonstrated that ORMV and gibberellic acid elevated P-miR164a activity. Accordingly, total mature miR164, precursor of miR164a and CUC1 mRNA (a miR164 target) levels increased after virus infection and interestingly the most severe virus (ORMV) produced the strongest promoter induction. Conclusion. This work shows for the first time that the alteration of miR pathways produced by viral infections possesses a transcriptional component. In addition, the degree of miR alteration correlates with virus severity since a more severe virus produces a stronger P-miR164a induction. © 2009 Bazzini et al; licensee BioMed Central Ltd. Fil:Bazzini, A.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Almasia, N.I. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Mongelli, V.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Maroniche, G.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rodriguez, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Distéfano, A.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Hopp, H.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Del Vas, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Asurmendi, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2009 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14712229_v9_n_p_Bazzini http://hdl.handle.net/20.500.12110/paper_14712229_v9_n_p_Bazzini
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Arabidopsis Brassica napus Chinese Rape Mosaic Virus Tobacco mosaic virus microRNA microRNA 164, Arabidopsis microRNA-164, Arabidopsis phytohormone plant RNA Arabidopsis article biology gene expression regulation genetics metabolism molecular cloning Mosaic virus physiology plant disease promoter region reporter gene transgenic plant virology Arabidopsis Cloning, Molecular Computational Biology Gene Expression Regulation, Developmental Gene Expression Regulation, Plant Genes, Reporter MicroRNAs Mosaic Viruses Plant Diseases Plant Growth Regulators Plants, Genetically Modified Promoter Regions, Genetic RNA, Plant
spellingShingle	Arabidopsis Brassica napus Chinese Rape Mosaic Virus Tobacco mosaic virus microRNA microRNA 164, Arabidopsis microRNA-164, Arabidopsis phytohormone plant RNA Arabidopsis article biology gene expression regulation genetics metabolism molecular cloning Mosaic virus physiology plant disease promoter region reporter gene transgenic plant virology Arabidopsis Cloning, Molecular Computational Biology Gene Expression Regulation, Developmental Gene Expression Regulation, Plant Genes, Reporter MicroRNAs Mosaic Viruses Plant Diseases Plant Growth Regulators Plants, Genetically Modified Promoter Regions, Genetic RNA, Plant Bazzini, Ariel Alejandro Almasia, Natalia Inés Mongelli, Vanesa Claudia Maroniche, Guillermo Andrés Rodriguez, María Cecilia Distéfano, Ana Julia Hopp, Horacio Esteban Del Vas, Mariana Asurmendi, Sebastián Virus infection elevates transcriptional activity of miR164a promoter in plants
topic_facet	Arabidopsis Brassica napus Chinese Rape Mosaic Virus Tobacco mosaic virus microRNA microRNA 164, Arabidopsis microRNA-164, Arabidopsis phytohormone plant RNA Arabidopsis article biology gene expression regulation genetics metabolism molecular cloning Mosaic virus physiology plant disease promoter region reporter gene transgenic plant virology Arabidopsis Cloning, Molecular Computational Biology Gene Expression Regulation, Developmental Gene Expression Regulation, Plant Genes, Reporter MicroRNAs Mosaic Viruses Plant Diseases Plant Growth Regulators Plants, Genetically Modified Promoter Regions, Genetic RNA, Plant
description	Background. Micro RNAs (miRs) constitute a large group of endogenous small RNAs that have crucial roles in many important plant functions. Virus infection and transgenic expression of viral proteins alter accumulation and activity of miRs and so far, most of the published evidence involves post-transcriptional regulations. Results. Using transgenic plants expressing a reporter gene under the promoter region of a characterized miR (P-miR164a), we monitored the reporter gene expression in different tissues and during Arabidopsis development. Strong expression was detected in both vascular tissues and hydathodes. P-miR164a activity was developmentally regulated in plants with a maximum expression at stages 1.12 to 5.1 (according to Boyes, 2001) along the transition from vegetative to reproductive growth. Upon quantification of P-miR164a-derived GUS activity after Tobacco mosaic virus Cg or Oilseed rape mosaic virus (ORMV) infection and after hormone treatments, we demonstrated that ORMV and gibberellic acid elevated P-miR164a activity. Accordingly, total mature miR164, precursor of miR164a and CUC1 mRNA (a miR164 target) levels increased after virus infection and interestingly the most severe virus (ORMV) produced the strongest promoter induction. Conclusion. This work shows for the first time that the alteration of miR pathways produced by viral infections possesses a transcriptional component. In addition, the degree of miR alteration correlates with virus severity since a more severe virus produces a stronger P-miR164a induction. © 2009 Bazzini et al; licensee BioMed Central Ltd.
author	Bazzini, Ariel Alejandro Almasia, Natalia Inés Mongelli, Vanesa Claudia Maroniche, Guillermo Andrés Rodriguez, María Cecilia Distéfano, Ana Julia Hopp, Horacio Esteban Del Vas, Mariana Asurmendi, Sebastián
author_facet	Bazzini, Ariel Alejandro Almasia, Natalia Inés Mongelli, Vanesa Claudia Maroniche, Guillermo Andrés Rodriguez, María Cecilia Distéfano, Ana Julia Hopp, Horacio Esteban Del Vas, Mariana Asurmendi, Sebastián
author_sort	Bazzini, Ariel Alejandro
title	Virus infection elevates transcriptional activity of miR164a promoter in plants
title_short	Virus infection elevates transcriptional activity of miR164a promoter in plants
title_full	Virus infection elevates transcriptional activity of miR164a promoter in plants
title_fullStr	Virus infection elevates transcriptional activity of miR164a promoter in plants
title_full_unstemmed	Virus infection elevates transcriptional activity of miR164a promoter in plants
title_sort	virus infection elevates transcriptional activity of mir164a promoter in plants
publishDate	2009
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14712229_v9_n_p_Bazzini http://hdl.handle.net/20.500.12110/paper_14712229_v9_n_p_Bazzini
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_version_	1768545479605354496

Virus infection elevates transcriptional activity of miR164a promoter in plants

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