Alfalfa snakin-1 prevents fungal colonization and probably coevolved with rhizobia

Background: The production of antimicrobial peptides is a common defense strategy of living cells against a wide range of pathogens. Plant snakin peptides inhibit bacterial and fungal growth at extremely low concentrations. However, little is known of their molecular and ecological characteristics,...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ayub, Nicolás Daniel, Fox, Ana Romina, Dieguez, María José, Berini, Carolina Andrea, Muschietti, Jorge P., Soto, Gabriela Cynthia
Publicado:	2014
Materias:	Alfalfa Antimicrobial peptides Evolution Innate immunity Land plants Snakin Embryophyta Medicago sativa antimicrobial cationic peptide vegetable protein alfalfa evolution gene expression genetics immunology metabolism microbiology physiology plant immunity Rhizobium symbiosis transgenic plant Antimicrobial Cationic Peptides Biological Evolution Gene Expression Plant Immunity Plant Proteins Plants, Genetically Modified Symbiosis
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14712229_v14_n1_p_Garcia http://hdl.handle.net/20.500.12110/paper_14712229_v14_n1_p_Garcia
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_14712229_v14_n1_p_Garcia
record_format	dspace
spelling	paper:paper_14712229_v14_n1_p_Garcia2023-06-08T16:17:17Z Alfalfa snakin-1 prevents fungal colonization and probably coevolved with rhizobia Ayub, Nicolás Daniel Fox, Ana Romina Dieguez, María José Berini, Carolina Andrea Muschietti, Jorge P. Soto, Gabriela Cynthia Alfalfa Antimicrobial peptides Evolution Innate immunity Land plants Snakin Embryophyta Medicago sativa antimicrobial cationic peptide vegetable protein alfalfa evolution gene expression genetics immunology metabolism microbiology physiology plant immunity Rhizobium symbiosis transgenic plant Antimicrobial Cationic Peptides Biological Evolution Gene Expression Medicago sativa Plant Immunity Plant Proteins Plants, Genetically Modified Rhizobium Symbiosis Background: The production of antimicrobial peptides is a common defense strategy of living cells against a wide range of pathogens. Plant snakin peptides inhibit bacterial and fungal growth at extremely low concentrations. However, little is known of their molecular and ecological characteristics, including origin, evolutionary equivalence, specific functions and activity against beneficial microbes. The aim of this study was to identify and characterize snakin-1 from alfalfa (MsSN1).Results: Phylogenetic analysis showed complete congruence between snakin-1 and plant trees. The antimicrobial activity of MsSN1 against bacterial and fungal pathogens of alfalfa was demonstrated in vitro and in vivo. Transgenic alfalfa overexpressing MsSN1 showed increased antimicrobial activity against virulent fungal strains. However, MsSN1 did not affect nitrogen-fixing bacterial strains only when these had an alfalfa origin.Conclusions: The results reported here suggest that snakin peptides have important and ancestral roles in land plant innate immunity. Our data indicate a coevolutionary process, in which alfalfa exerts a selection pressure for resistance to MsSN1 on rhizobial bacteria. The increased antimicrobial activity against virulent fungal strains without altering the nitrogen-fixing symbiosis observed in MsSN1-overexpressing alfalfa transgenic plants opens the way to the production of effective legume transgenic cultivars for biotic stress resistance. © 2014 García et al.; licensee BioMed Central Ltd. Fil:Ayub, N.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Fox, A.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Diéguez, M.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Berini, C.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Muschietti, J.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Soto, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2014 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14712229_v14_n1_p_Garcia http://hdl.handle.net/20.500.12110/paper_14712229_v14_n1_p_Garcia
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Alfalfa Antimicrobial peptides Evolution Innate immunity Land plants Snakin Embryophyta Medicago sativa antimicrobial cationic peptide vegetable protein alfalfa evolution gene expression genetics immunology metabolism microbiology physiology plant immunity Rhizobium symbiosis transgenic plant Antimicrobial Cationic Peptides Biological Evolution Gene Expression Medicago sativa Plant Immunity Plant Proteins Plants, Genetically Modified Rhizobium Symbiosis
spellingShingle	Alfalfa Antimicrobial peptides Evolution Innate immunity Land plants Snakin Embryophyta Medicago sativa antimicrobial cationic peptide vegetable protein alfalfa evolution gene expression genetics immunology metabolism microbiology physiology plant immunity Rhizobium symbiosis transgenic plant Antimicrobial Cationic Peptides Biological Evolution Gene Expression Medicago sativa Plant Immunity Plant Proteins Plants, Genetically Modified Rhizobium Symbiosis Ayub, Nicolás Daniel Fox, Ana Romina Dieguez, María José Berini, Carolina Andrea Muschietti, Jorge P. Soto, Gabriela Cynthia Alfalfa snakin-1 prevents fungal colonization and probably coevolved with rhizobia
topic_facet	Alfalfa Antimicrobial peptides Evolution Innate immunity Land plants Snakin Embryophyta Medicago sativa antimicrobial cationic peptide vegetable protein alfalfa evolution gene expression genetics immunology metabolism microbiology physiology plant immunity Rhizobium symbiosis transgenic plant Antimicrobial Cationic Peptides Biological Evolution Gene Expression Medicago sativa Plant Immunity Plant Proteins Plants, Genetically Modified Rhizobium Symbiosis
description	Background: The production of antimicrobial peptides is a common defense strategy of living cells against a wide range of pathogens. Plant snakin peptides inhibit bacterial and fungal growth at extremely low concentrations. However, little is known of their molecular and ecological characteristics, including origin, evolutionary equivalence, specific functions and activity against beneficial microbes. The aim of this study was to identify and characterize snakin-1 from alfalfa (MsSN1).Results: Phylogenetic analysis showed complete congruence between snakin-1 and plant trees. The antimicrobial activity of MsSN1 against bacterial and fungal pathogens of alfalfa was demonstrated in vitro and in vivo. Transgenic alfalfa overexpressing MsSN1 showed increased antimicrobial activity against virulent fungal strains. However, MsSN1 did not affect nitrogen-fixing bacterial strains only when these had an alfalfa origin.Conclusions: The results reported here suggest that snakin peptides have important and ancestral roles in land plant innate immunity. Our data indicate a coevolutionary process, in which alfalfa exerts a selection pressure for resistance to MsSN1 on rhizobial bacteria. The increased antimicrobial activity against virulent fungal strains without altering the nitrogen-fixing symbiosis observed in MsSN1-overexpressing alfalfa transgenic plants opens the way to the production of effective legume transgenic cultivars for biotic stress resistance. © 2014 García et al.; licensee BioMed Central Ltd.
author	Ayub, Nicolás Daniel Fox, Ana Romina Dieguez, María José Berini, Carolina Andrea Muschietti, Jorge P. Soto, Gabriela Cynthia
author_facet	Ayub, Nicolás Daniel Fox, Ana Romina Dieguez, María José Berini, Carolina Andrea Muschietti, Jorge P. Soto, Gabriela Cynthia
author_sort	Ayub, Nicolás Daniel
title	Alfalfa snakin-1 prevents fungal colonization and probably coevolved with rhizobia
title_short	Alfalfa snakin-1 prevents fungal colonization and probably coevolved with rhizobia
title_full	Alfalfa snakin-1 prevents fungal colonization and probably coevolved with rhizobia
title_fullStr	Alfalfa snakin-1 prevents fungal colonization and probably coevolved with rhizobia
title_full_unstemmed	Alfalfa snakin-1 prevents fungal colonization and probably coevolved with rhizobia
title_sort	alfalfa snakin-1 prevents fungal colonization and probably coevolved with rhizobia
publishDate	2014
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14712229_v14_n1_p_Garcia http://hdl.handle.net/20.500.12110/paper_14712229_v14_n1_p_Garcia
work_keys_str_mv	AT ayubnicolasdaniel alfalfasnakin1preventsfungalcolonizationandprobablycoevolvedwithrhizobia AT foxanaromina alfalfasnakin1preventsfungalcolonizationandprobablycoevolvedwithrhizobia AT dieguezmariajose alfalfasnakin1preventsfungalcolonizationandprobablycoevolvedwithrhizobia AT berinicarolinaandrea alfalfasnakin1preventsfungalcolonizationandprobablycoevolvedwithrhizobia AT muschiettijorgep alfalfasnakin1preventsfungalcolonizationandprobablycoevolvedwithrhizobia AT sotogabrielacynthia alfalfasnakin1preventsfungalcolonizationandprobablycoevolvedwithrhizobia
_version_	1768543864065359872

Alfalfa snakin-1 prevents fungal colonization and probably coevolved with rhizobia

Ejemplares similares