Pairing and recombination features during meiosis in Cebus paraguayanus (Primates: Platyrrhini)

Background: Among neotropical Primates, the Cai monkey Cebus paraguayanus (CPA) presents long, conserved chromosome syntenies with the human karyotype (HSA) as well as numerous C+ blocks in different chromosome pairs. In this study, immunofluorescence (IF) against two proteins of the Synaptonemal Co...

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Detalles Bibliográficos
Publicado: 2009
Materias:
BRCA1 protein
chromosome protein
protein MLH1
protein Rec8
protein SYCPI
replication factor A
unclassified drug
cell cycle protein
animal cell
article
autosome
Cebus paraguayanus
cell nucleus
chromosome bivalent
chromosome pairing
genetic recombination
heterochromatin
immunofluorescence
meiosis
nonhuman
pachytene
primate
protein structure
animal
Cebidae
chromosome
fluorescence in situ hybridization
genetics
male
metabolism
spermatocyte
Cebus
Platyrrhini
Primates
Synapsis
Animals
Cell Cycle Proteins
Chromosome Pairing
Chromosomes
Heterochromatin
In Situ Hybridization, Fluorescence
Male
Meiosis
Recombination, Genetic
Spermatocytes
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14712156_v10_n_p_GarciaCruz
http://hdl.handle.net/20.500.12110/paper_14712156_v10_n_p_GarciaCruz
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
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spelling paper:paper_14712156_v10_n_p_GarciaCruz2023-06-08T16:17:13Z Pairing and recombination features during meiosis in Cebus paraguayanus (Primates: Platyrrhini) BRCA1 protein chromosome protein protein MLH1 protein Rec8 protein SYCPI replication factor A unclassified drug cell cycle protein animal cell article autosome Cebus paraguayanus cell nucleus chromosome bivalent chromosome pairing genetic recombination heterochromatin immunofluorescence meiosis nonhuman pachytene primate protein structure animal Cebidae chromosome fluorescence in situ hybridization genetics male metabolism spermatocyte Cebus Platyrrhini Primates Synapsis Animals Cebus Cell Cycle Proteins Chromosome Pairing Chromosomes Heterochromatin In Situ Hybridization, Fluorescence Male Meiosis Recombination, Genetic Spermatocytes Background: Among neotropical Primates, the Cai monkey Cebus paraguayanus (CPA) presents long, conserved chromosome syntenies with the human karyotype (HSA) as well as numerous C+ blocks in different chromosome pairs. In this study, immunofluorescence (IF) against two proteins of the Synaptonemal Complex (SC), namely REC8 and SYCP1, two recombination protein markers (RPA and MLH1), and one protein involved in the pachytene checkpoint machinery (BRCA1) was performed in CPA spermatocytes in order to analyze chromosome meiotic behavior in detail. Results: Although in the vast majority of pachytene cells all autosomeswere paired and synapsed, in a small number of nuclei the heterochromatic C-positive terminal region of bivalent 11 remained unpaired. The analysis of 75 CPA cells at pachytene revealed a mean of 43.22 MLH1 foci per nucleus and 1.07 MLH1 foci in each CPA bivalent 11, always positioned in the region homologous to HSA chromosome 21. Conclusion: Our results suggest that C blocks undergo delayed pairing and synapsis, although they do not interfere with the general progress of pairing and synapsis. © 2009 García-Cruz et al; licensee BioMed Central Ltd. 2009 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14712156_v10_n_p_GarciaCruz http://hdl.handle.net/20.500.12110/paper_14712156_v10_n_p_GarciaCruz
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic BRCA1 protein
chromosome protein
protein MLH1
protein Rec8
protein SYCPI
replication factor A
unclassified drug
cell cycle protein
animal cell
article
autosome
Cebus paraguayanus
cell nucleus
chromosome bivalent
chromosome pairing
genetic recombination
heterochromatin
immunofluorescence
meiosis
nonhuman
pachytene
primate
protein structure
animal
Cebidae
chromosome
fluorescence in situ hybridization
genetics
male
metabolism
spermatocyte
Cebus
Platyrrhini
Primates
Synapsis
Animals
Cebus
Cell Cycle Proteins
Chromosome Pairing
Chromosomes
Heterochromatin
In Situ Hybridization, Fluorescence
Male
Meiosis
Recombination, Genetic
Spermatocytes
spellingShingle BRCA1 protein
chromosome protein
protein MLH1
protein Rec8
protein SYCPI
replication factor A
unclassified drug
cell cycle protein
animal cell
article
autosome
Cebus paraguayanus
cell nucleus
chromosome bivalent
chromosome pairing
genetic recombination
heterochromatin
immunofluorescence
meiosis
nonhuman
pachytene
primate
protein structure
animal
Cebidae
chromosome
fluorescence in situ hybridization
genetics
male
metabolism
spermatocyte
Cebus
Platyrrhini
Primates
Synapsis
Animals
Cebus
Cell Cycle Proteins
Chromosome Pairing
Chromosomes
Heterochromatin
In Situ Hybridization, Fluorescence
Male
Meiosis
Recombination, Genetic
Spermatocytes
Pairing and recombination features during meiosis in Cebus paraguayanus (Primates: Platyrrhini)
topic_facet BRCA1 protein
chromosome protein
protein MLH1
protein Rec8
protein SYCPI
replication factor A
unclassified drug
cell cycle protein
animal cell
article
autosome
Cebus paraguayanus
cell nucleus
chromosome bivalent
chromosome pairing
genetic recombination
heterochromatin
immunofluorescence
meiosis
nonhuman
pachytene
primate
protein structure
animal
Cebidae
chromosome
fluorescence in situ hybridization
genetics
male
metabolism
spermatocyte
Cebus
Platyrrhini
Primates
Synapsis
Animals
Cebus
Cell Cycle Proteins
Chromosome Pairing
Chromosomes
Heterochromatin
In Situ Hybridization, Fluorescence
Male
Meiosis
Recombination, Genetic
Spermatocytes
description Background: Among neotropical Primates, the Cai monkey Cebus paraguayanus (CPA) presents long, conserved chromosome syntenies with the human karyotype (HSA) as well as numerous C+ blocks in different chromosome pairs. In this study, immunofluorescence (IF) against two proteins of the Synaptonemal Complex (SC), namely REC8 and SYCP1, two recombination protein markers (RPA and MLH1), and one protein involved in the pachytene checkpoint machinery (BRCA1) was performed in CPA spermatocytes in order to analyze chromosome meiotic behavior in detail. Results: Although in the vast majority of pachytene cells all autosomeswere paired and synapsed, in a small number of nuclei the heterochromatic C-positive terminal region of bivalent 11 remained unpaired. The analysis of 75 CPA cells at pachytene revealed a mean of 43.22 MLH1 foci per nucleus and 1.07 MLH1 foci in each CPA bivalent 11, always positioned in the region homologous to HSA chromosome 21. Conclusion: Our results suggest that C blocks undergo delayed pairing and synapsis, although they do not interfere with the general progress of pairing and synapsis. © 2009 García-Cruz et al; licensee BioMed Central Ltd.
title Pairing and recombination features during meiosis in Cebus paraguayanus (Primates: Platyrrhini)
title_short Pairing and recombination features during meiosis in Cebus paraguayanus (Primates: Platyrrhini)
title_full Pairing and recombination features during meiosis in Cebus paraguayanus (Primates: Platyrrhini)
title_fullStr Pairing and recombination features during meiosis in Cebus paraguayanus (Primates: Platyrrhini)
title_full_unstemmed Pairing and recombination features during meiosis in Cebus paraguayanus (Primates: Platyrrhini)
title_sort pairing and recombination features during meiosis in cebus paraguayanus (primates: platyrrhini)
publishDate 2009
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14712156_v10_n_p_GarciaCruz
http://hdl.handle.net/20.500.12110/paper_14712156_v10_n_p_GarciaCruz
_version_ 1768545753640206336

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