Murine sperm capacitation, oocyte penetration and decondensation following moderate alcohol intake
Male chronic alcohol abuse causes testicular failure and infertility. We analyzed the effects of moderate sub-chronic alcohol intake on sperm morphology, capacitation, fertilization and sperm head decondensation. CF-1 male mice were administered 15% ethanol in drinking water for 15 days; control mic...
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2018
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14701626_v155_n6_p529_Sanchez http://hdl.handle.net/20.500.12110/paper_14701626_v155_n6_p529_Sanchez |
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paper:paper_14701626_v155_n6_p529_Sanchez2023-06-08T16:17:06Z Murine sperm capacitation, oocyte penetration and decondensation following moderate alcohol intake alcohol progesterone tyrosine alcohol topical antiinfective agent acrosome reaction alcohol consumption animal cell animal experiment Article controlled study exocytosis in vitro fertilization in vitro study male mouse nonhuman oocyte oocyte penetration priority journal protein phosphorylation reproduction spermatozoon capacitation spermatozoon head spermatozoon motility animal drug effect fertility pathology spermatozoon capacitation Acrosome Reaction Animals Anti-Infective Agents, Local Ethanol Fertilization in Vitro Male Mice Oocytes Sperm Capacitation Sperm-Ovum Interactions Male chronic alcohol abuse causes testicular failure and infertility. We analyzed the effects of moderate sub-chronic alcohol intake on sperm morphology, capacitation, fertilization and sperm head decondensation. CF-1 male mice were administered 15% ethanol in drinking water for 15 days; control mice received ethanol-free water. Similar patterns of tyrosine phosphorylation were observed in capacitated spermatozoa of control and treated males. Percentage of hyperactivation (H) and spontaneous (SAR) and progesterone-induced (IAR) acrosome reaction significantly decreased at 120 and 150min of capacitation in treated males compared to controls (H: 14.1±2.5 vs 23.7±2.6, P<0.05; SAR-T120min: 17.9±2.5 vs 32.9±4.1, P<0.01; IAR-150min: 43.3±3.5 vs 73.1±1.1, P<0.001, n=6). During in vitro fertilization (2.5, 3.5 and 4.5h post-insemination), there was an increased percentage of fertilized oocytes (with a decondensed sperm head and one or two pronuclei) in treated males (P<0.001, n=7). After 60min of in vitro decondensation with glutathione plus heparin, the percentage of decondensed sperm heads was significantly higher in treated males than in controls (mean±s.d.: 57.1±5.6 vs 48.3±4.5, P<0.05, n=5). The percentage of morphologically normal sperm heads was significantly decreased in treated males with respect to controls (P<0.001, n=9). These results show that short-term moderate alcohol consumption in outbred mice affect sperm morphology, hyperactivation, acrosomal exocytosis, and the dynamics of in vitro fertilization and in vitro sperm nuclear decondensation. © 2018 Society for Reproduction and Fertility. 2018 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14701626_v155_n6_p529_Sanchez http://hdl.handle.net/20.500.12110/paper_14701626_v155_n6_p529_Sanchez |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
alcohol progesterone tyrosine alcohol topical antiinfective agent acrosome reaction alcohol consumption animal cell animal experiment Article controlled study exocytosis in vitro fertilization in vitro study male mouse nonhuman oocyte oocyte penetration priority journal protein phosphorylation reproduction spermatozoon capacitation spermatozoon head spermatozoon motility animal drug effect fertility pathology spermatozoon capacitation Acrosome Reaction Animals Anti-Infective Agents, Local Ethanol Fertilization in Vitro Male Mice Oocytes Sperm Capacitation Sperm-Ovum Interactions |
| spellingShingle |
alcohol progesterone tyrosine alcohol topical antiinfective agent acrosome reaction alcohol consumption animal cell animal experiment Article controlled study exocytosis in vitro fertilization in vitro study male mouse nonhuman oocyte oocyte penetration priority journal protein phosphorylation reproduction spermatozoon capacitation spermatozoon head spermatozoon motility animal drug effect fertility pathology spermatozoon capacitation Acrosome Reaction Animals Anti-Infective Agents, Local Ethanol Fertilization in Vitro Male Mice Oocytes Sperm Capacitation Sperm-Ovum Interactions Murine sperm capacitation, oocyte penetration and decondensation following moderate alcohol intake |
| topic_facet |
alcohol progesterone tyrosine alcohol topical antiinfective agent acrosome reaction alcohol consumption animal cell animal experiment Article controlled study exocytosis in vitro fertilization in vitro study male mouse nonhuman oocyte oocyte penetration priority journal protein phosphorylation reproduction spermatozoon capacitation spermatozoon head spermatozoon motility animal drug effect fertility pathology spermatozoon capacitation Acrosome Reaction Animals Anti-Infective Agents, Local Ethanol Fertilization in Vitro Male Mice Oocytes Sperm Capacitation Sperm-Ovum Interactions |
| description |
Male chronic alcohol abuse causes testicular failure and infertility. We analyzed the effects of moderate sub-chronic alcohol intake on sperm morphology, capacitation, fertilization and sperm head decondensation. CF-1 male mice were administered 15% ethanol in drinking water for 15 days; control mice received ethanol-free water. Similar patterns of tyrosine phosphorylation were observed in capacitated spermatozoa of control and treated males. Percentage of hyperactivation (H) and spontaneous (SAR) and progesterone-induced (IAR) acrosome reaction significantly decreased at 120 and 150min of capacitation in treated males compared to controls (H: 14.1±2.5 vs 23.7±2.6, P<0.05; SAR-T120min: 17.9±2.5 vs 32.9±4.1, P<0.01; IAR-150min: 43.3±3.5 vs 73.1±1.1, P<0.001, n=6). During in vitro fertilization (2.5, 3.5 and 4.5h post-insemination), there was an increased percentage of fertilized oocytes (with a decondensed sperm head and one or two pronuclei) in treated males (P<0.001, n=7). After 60min of in vitro decondensation with glutathione plus heparin, the percentage of decondensed sperm heads was significantly higher in treated males than in controls (mean±s.d.: 57.1±5.6 vs 48.3±4.5, P<0.05, n=5). The percentage of morphologically normal sperm heads was significantly decreased in treated males with respect to controls (P<0.001, n=9). These results show that short-term moderate alcohol consumption in outbred mice affect sperm morphology, hyperactivation, acrosomal exocytosis, and the dynamics of in vitro fertilization and in vitro sperm nuclear decondensation. © 2018 Society for Reproduction and Fertility. |
| title |
Murine sperm capacitation, oocyte penetration and decondensation following moderate alcohol intake |
| title_short |
Murine sperm capacitation, oocyte penetration and decondensation following moderate alcohol intake |
| title_full |
Murine sperm capacitation, oocyte penetration and decondensation following moderate alcohol intake |
| title_fullStr |
Murine sperm capacitation, oocyte penetration and decondensation following moderate alcohol intake |
| title_full_unstemmed |
Murine sperm capacitation, oocyte penetration and decondensation following moderate alcohol intake |
| title_sort |
murine sperm capacitation, oocyte penetration and decondensation following moderate alcohol intake |
| publishDate |
2018 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14701626_v155_n6_p529_Sanchez http://hdl.handle.net/20.500.12110/paper_14701626_v155_n6_p529_Sanchez |
| _version_ |
1768546643829850112 |