Expression of fragile X mental retardation protein and Fmr1 mRNA during folliculogenesis in the rat
Fragile X mental retardation protein (FMRP) belongs to a small family of RNA-binding proteins. Its absence or inactivity is responsible for fragile X syndrome, the most common cause of inherited mental retardation. Despite its ubiquitous expression, FMRP function and expression remain almost underst...
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2013
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14701626_v145_n4_p335_Ferder http://hdl.handle.net/20.500.12110/paper_14701626_v145_n4_p335_Ferder |
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paper:paper_14701626_v145_n4_p335_Ferder2023-06-08T16:17:05Z Expression of fragile X mental retardation protein and Fmr1 mRNA during folliculogenesis in the rat Parborell, M.Fernanda A. Sundblad, Victoria Charreau, Eduardo Hernán Tesone, Marta fragile X mental retardation protein messenger RNA animal experiment animal tissue article brain cytoplasm female germ cell granulosa cell immunohistochemistry nonhuman ovary follicle ovary follicle development priority journal protein expression rat testis theca cell Animals Female Fragile X Mental Retardation Protein Ovarian Follicle Protein Isoforms Rats Rats, Sprague-Dawley RNA, Messenger Fragile X mental retardation protein (FMRP) belongs to a small family of RNA-binding proteins. Its absence or inactivity is responsible for fragile X syndrome, the most common cause of inherited mental retardation. Despite its ubiquitous expression, FMRP function and expression remain almost understudied in non-neuronal tissues, though previous studies on germline development during oogenesis may suggest a special function of this protein also in ovarian tissue. In addition, the well-documented association of FMR1 premutation state with fragile X-related premature ovarian insufficiency adds interest to the role of FMRP in ovarian physiology. The aim of the present work was to investigate the expression of Fmr1 mRNA and its protein, FMRP, at different stages of rat follicular development. By immunohistochemical studies we demonstrated FMRP expression in granulosa, theca and germ cells in all stages of follicular development. In addition, changes in Fmr1 expression, both at the protein and mRNA levels, were observed. FMRP levels increased upon follicular development while preantral and early antral follicles presented similar levels of Fmr1 transcripts with decreased expression in preovulatory follicles. These observations suggest that Fmr1 expression in the ovary is regulated at different and perhaps independent levels. In addition, our results show expression of at least four different isoforms of FMRP during all stages of follicular growth with expression patterns that differ from those observed in brain and testis. Our study shows a regulated expression of Fmr1, both at mRNA and protein levels, during rat follicular development. © 2013 Society for Reproduction and Fertility. Fil:Parborell, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Sundblad, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Charreau, E.H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Tesone, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2013 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14701626_v145_n4_p335_Ferder http://hdl.handle.net/20.500.12110/paper_14701626_v145_n4_p335_Ferder |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
fragile X mental retardation protein messenger RNA animal experiment animal tissue article brain cytoplasm female germ cell granulosa cell immunohistochemistry nonhuman ovary follicle ovary follicle development priority journal protein expression rat testis theca cell Animals Female Fragile X Mental Retardation Protein Ovarian Follicle Protein Isoforms Rats Rats, Sprague-Dawley RNA, Messenger |
| spellingShingle |
fragile X mental retardation protein messenger RNA animal experiment animal tissue article brain cytoplasm female germ cell granulosa cell immunohistochemistry nonhuman ovary follicle ovary follicle development priority journal protein expression rat testis theca cell Animals Female Fragile X Mental Retardation Protein Ovarian Follicle Protein Isoforms Rats Rats, Sprague-Dawley RNA, Messenger Parborell, M.Fernanda A. Sundblad, Victoria Charreau, Eduardo Hernán Tesone, Marta Expression of fragile X mental retardation protein and Fmr1 mRNA during folliculogenesis in the rat |
| topic_facet |
fragile X mental retardation protein messenger RNA animal experiment animal tissue article brain cytoplasm female germ cell granulosa cell immunohistochemistry nonhuman ovary follicle ovary follicle development priority journal protein expression rat testis theca cell Animals Female Fragile X Mental Retardation Protein Ovarian Follicle Protein Isoforms Rats Rats, Sprague-Dawley RNA, Messenger |
| description |
Fragile X mental retardation protein (FMRP) belongs to a small family of RNA-binding proteins. Its absence or inactivity is responsible for fragile X syndrome, the most common cause of inherited mental retardation. Despite its ubiquitous expression, FMRP function and expression remain almost understudied in non-neuronal tissues, though previous studies on germline development during oogenesis may suggest a special function of this protein also in ovarian tissue. In addition, the well-documented association of FMR1 premutation state with fragile X-related premature ovarian insufficiency adds interest to the role of FMRP in ovarian physiology. The aim of the present work was to investigate the expression of Fmr1 mRNA and its protein, FMRP, at different stages of rat follicular development. By immunohistochemical studies we demonstrated FMRP expression in granulosa, theca and germ cells in all stages of follicular development. In addition, changes in Fmr1 expression, both at the protein and mRNA levels, were observed. FMRP levels increased upon follicular development while preantral and early antral follicles presented similar levels of Fmr1 transcripts with decreased expression in preovulatory follicles. These observations suggest that Fmr1 expression in the ovary is regulated at different and perhaps independent levels. In addition, our results show expression of at least four different isoforms of FMRP during all stages of follicular growth with expression patterns that differ from those observed in brain and testis. Our study shows a regulated expression of Fmr1, both at mRNA and protein levels, during rat follicular development. © 2013 Society for Reproduction and Fertility. |
| author |
Parborell, M.Fernanda A. Sundblad, Victoria Charreau, Eduardo Hernán Tesone, Marta |
| author_facet |
Parborell, M.Fernanda A. Sundblad, Victoria Charreau, Eduardo Hernán Tesone, Marta |
| author_sort |
Parborell, M.Fernanda A. |
| title |
Expression of fragile X mental retardation protein and Fmr1 mRNA during folliculogenesis in the rat |
| title_short |
Expression of fragile X mental retardation protein and Fmr1 mRNA during folliculogenesis in the rat |
| title_full |
Expression of fragile X mental retardation protein and Fmr1 mRNA during folliculogenesis in the rat |
| title_fullStr |
Expression of fragile X mental retardation protein and Fmr1 mRNA during folliculogenesis in the rat |
| title_full_unstemmed |
Expression of fragile X mental retardation protein and Fmr1 mRNA during folliculogenesis in the rat |
| title_sort |
expression of fragile x mental retardation protein and fmr1 mrna during folliculogenesis in the rat |
| publishDate |
2013 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14701626_v145_n4_p335_Ferder http://hdl.handle.net/20.500.12110/paper_14701626_v145_n4_p335_Ferder |
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| _version_ |
1768546643647397888 |