Expression of caspase-2, -3, -8 and -9 proteins and enzyme activity in the corpus luteum of the rat at diferent stages during the natural estrous cycle
Apoptosis is associated with the regression of the corpus luteum (CL) in many species. Since caspases play a central role in apoptosis, we studied several initiators (-2, -8, and -9) and the main effector (-3) caspase in the CL during the estrous cycle of the rat. Two different populations of CL (ol...
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2006
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14701626_v132_n3_p465_Peluffo http://hdl.handle.net/20.500.12110/paper_14701626_v132_n3_p465_Peluffo |
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paper:paper_14701626_v132_n3_p465_Peluffo2023-06-08T16:17:03Z Expression of caspase-2, -3, -8 and -9 proteins and enzyme activity in the corpus luteum of the rat at diferent stages during the natural estrous cycle caspase 2 caspase 3 caspase 8 caspase 9 cytochrome P450 progesterone animal tissue apoptosis article controlled study corpus luteum diestrus endothelium cell enzyme activity estrus cycle female immunohistochemistry luteal cell luteal phase nick end labeling nonhuman priority journal protein analysis protein content protein expression protein function rat statistical significance Western blotting Animals Apoptosis Blotting, Western Caspase 2 Caspase 3 Caspase 8 Caspase 9 Caspases Cholesterol Side-Chain Cleavage Enzyme Corpus Luteum Estrous Cycle Female Immunohistochemistry In Situ Nick-End Labeling Progesterone Rats Rats, Sprague-Dawley Apoptosis is associated with the regression of the corpus luteum (CL) in many species. Since caspases play a central role in apoptosis, we studied several initiators (-2, -8, and -9) and the main effector (-3) caspase in the CL during the estrous cycle of the rat. Two different populations of CL (old and new) were identified on ovaries at estrus and diestrus II (DII). Diminished (P<0.05) luteal progesterone content and P450scc levels suggested that functional luteolysis occurred between the new CL at DII and old CL at estrus, whereas the decline (P<0.05) in luteal weight indicated that structural regression was occurring between old CL at estrus to DII. Immunostaining for caspase-2 in luteal and endothelial cells appeared to increase as the luteal phase progressed, peaking at DII in the old CL. However, caspase-8 and -9 immunostaining showed little change with a slight increase at estrus in the old population. Notably, caspase-3 staining appeared to peak at DII in the new CL. Enzyme activity of caspase-9 increased (P<0.05) in the new CL at DII, followed by that of caspase-2 and -3 in old CL at estrus. Caspase-8 activity did not change at any stage. The number of apoptotic cells increased at DII in the old CL. These results suggest an important role for this protease family during early events of luteolysis in the rat estrous cycle. © 2006 Society for Reproduction and Fertility. 2006 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14701626_v132_n3_p465_Peluffo http://hdl.handle.net/20.500.12110/paper_14701626_v132_n3_p465_Peluffo |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
caspase 2 caspase 3 caspase 8 caspase 9 cytochrome P450 progesterone animal tissue apoptosis article controlled study corpus luteum diestrus endothelium cell enzyme activity estrus cycle female immunohistochemistry luteal cell luteal phase nick end labeling nonhuman priority journal protein analysis protein content protein expression protein function rat statistical significance Western blotting Animals Apoptosis Blotting, Western Caspase 2 Caspase 3 Caspase 8 Caspase 9 Caspases Cholesterol Side-Chain Cleavage Enzyme Corpus Luteum Estrous Cycle Female Immunohistochemistry In Situ Nick-End Labeling Progesterone Rats Rats, Sprague-Dawley |
| spellingShingle |
caspase 2 caspase 3 caspase 8 caspase 9 cytochrome P450 progesterone animal tissue apoptosis article controlled study corpus luteum diestrus endothelium cell enzyme activity estrus cycle female immunohistochemistry luteal cell luteal phase nick end labeling nonhuman priority journal protein analysis protein content protein expression protein function rat statistical significance Western blotting Animals Apoptosis Blotting, Western Caspase 2 Caspase 3 Caspase 8 Caspase 9 Caspases Cholesterol Side-Chain Cleavage Enzyme Corpus Luteum Estrous Cycle Female Immunohistochemistry In Situ Nick-End Labeling Progesterone Rats Rats, Sprague-Dawley Expression of caspase-2, -3, -8 and -9 proteins and enzyme activity in the corpus luteum of the rat at diferent stages during the natural estrous cycle |
| topic_facet |
caspase 2 caspase 3 caspase 8 caspase 9 cytochrome P450 progesterone animal tissue apoptosis article controlled study corpus luteum diestrus endothelium cell enzyme activity estrus cycle female immunohistochemistry luteal cell luteal phase nick end labeling nonhuman priority journal protein analysis protein content protein expression protein function rat statistical significance Western blotting Animals Apoptosis Blotting, Western Caspase 2 Caspase 3 Caspase 8 Caspase 9 Caspases Cholesterol Side-Chain Cleavage Enzyme Corpus Luteum Estrous Cycle Female Immunohistochemistry In Situ Nick-End Labeling Progesterone Rats Rats, Sprague-Dawley |
| description |
Apoptosis is associated with the regression of the corpus luteum (CL) in many species. Since caspases play a central role in apoptosis, we studied several initiators (-2, -8, and -9) and the main effector (-3) caspase in the CL during the estrous cycle of the rat. Two different populations of CL (old and new) were identified on ovaries at estrus and diestrus II (DII). Diminished (P<0.05) luteal progesterone content and P450scc levels suggested that functional luteolysis occurred between the new CL at DII and old CL at estrus, whereas the decline (P<0.05) in luteal weight indicated that structural regression was occurring between old CL at estrus to DII. Immunostaining for caspase-2 in luteal and endothelial cells appeared to increase as the luteal phase progressed, peaking at DII in the old CL. However, caspase-8 and -9 immunostaining showed little change with a slight increase at estrus in the old population. Notably, caspase-3 staining appeared to peak at DII in the new CL. Enzyme activity of caspase-9 increased (P<0.05) in the new CL at DII, followed by that of caspase-2 and -3 in old CL at estrus. Caspase-8 activity did not change at any stage. The number of apoptotic cells increased at DII in the old CL. These results suggest an important role for this protease family during early events of luteolysis in the rat estrous cycle. © 2006 Society for Reproduction and Fertility. |
| title |
Expression of caspase-2, -3, -8 and -9 proteins and enzyme activity in the corpus luteum of the rat at diferent stages during the natural estrous cycle |
| title_short |
Expression of caspase-2, -3, -8 and -9 proteins and enzyme activity in the corpus luteum of the rat at diferent stages during the natural estrous cycle |
| title_full |
Expression of caspase-2, -3, -8 and -9 proteins and enzyme activity in the corpus luteum of the rat at diferent stages during the natural estrous cycle |
| title_fullStr |
Expression of caspase-2, -3, -8 and -9 proteins and enzyme activity in the corpus luteum of the rat at diferent stages during the natural estrous cycle |
| title_full_unstemmed |
Expression of caspase-2, -3, -8 and -9 proteins and enzyme activity in the corpus luteum of the rat at diferent stages during the natural estrous cycle |
| title_sort |
expression of caspase-2, -3, -8 and -9 proteins and enzyme activity in the corpus luteum of the rat at diferent stages during the natural estrous cycle |
| publishDate |
2006 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14701626_v132_n3_p465_Peluffo http://hdl.handle.net/20.500.12110/paper_14701626_v132_n3_p465_Peluffo |
| _version_ |
1768545159785480192 |