Replicability of dominant bacterial populations after long-term surfactant-enrichment in lab-scale activated sludge
Bacterial communities were examined in replicate lab-scale activated sludge reactors after a period of several months of enrichment with non-ionic nonylphenol ethoxylate (NPE) surfactants. Four sequential batch reactors were fed with synthetic sewage, two of which received additionally NPE. Small su...
Guardado en:
| Autores principales: | , , |
|---|---|
| Publicado: |
2006
|
| Materias: | |
| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14622912_v8_n4_p625_Lozada http://hdl.handle.net/20.500.12110/paper_14622912_v8_n4_p625_Lozada |
| Aporte de: |
| id |
paper:paper_14622912_v8_n4_p625_Lozada |
|---|---|
| record_format |
dspace |
| spelling |
paper:paper_14622912_v8_n4_p625_Lozada2023-06-08T16:16:14Z Replicability of dominant bacterial populations after long-term surfactant-enrichment in lab-scale activated sludge Figuerola, Eva Lucía Margarita Itria, Raúl Fabio Erijman, Leonardo bacterial RNA ethylene glycol derivative ribosome DNA RNA 16S surfactant terics article bacterium bioreactor classification fluorescence in situ hybridization genetics growth, development and aging instrumentation methodology microbiology phylogeny reverse transcription polymerase chain reaction sewage two dimensional gel electrophoresis water management Bacteria Bioreactors DNA, Ribosomal Electrophoresis, Gel, Two-Dimensional Ethylene Glycols In Situ Hybridization, Fluorescence Phylogeny Reverse Transcriptase Polymerase Chain Reaction RNA, Bacterial RNA, Ribosomal, 16S Sewage Soil Microbiology Surface-Active Agents Water Purification Acidobacteria Alphaproteobacteria Bacteria (microorganisms) Betaproteobacteria Burkholderiales Gammaproteobacteria Sphingomonas Bacterial communities were examined in replicate lab-scale activated sludge reactors after a period of several months of enrichment with non-ionic nonylphenol ethoxylate (NPE) surfactants. Four sequential batch reactors were fed with synthetic sewage, two of which received additionally NPE. Small subunit rDNA-derived denaturing gel gradient electrophoresis (DGGE) profiles and 16S rDNA clone libraries were dominated by clones of Gammaproteobacteria class. Sequences of the other codominant rDNA phylotypes observed only in DGGE from NPE-amended reactors were, respectively, associated with the Group III of the Acidobacteria phylum. Intriguingly, 16S rRNA content from abundant Gammaproteobacteria cells was unexpectedly low. In addition to Acidobacteria, rRNA-derived DGGE profiles were dominated by members of the order Burkholderiales (of the Betaproteobacteria) and of the genus Sphingomonas (a member of the Alphaproteobacteria). Specific oligonucleotide probes for the selected ribotypes were designed and applied for quantitative real time polymerase chain reaction and fluorescence in situ hybridization, confirming their dominance in treated reactors. The parallel abundance of unique phylotypes in replicate reactors implies a distinctive selection of dominant organisms, which are better adapted to specialized niches in the highly selective environment. © 2005 Society for Applied Microbiology and Blackwell Publishing Ltd. Fil:Figuerola, E.L.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Itria, R.F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Erijman, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2006 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14622912_v8_n4_p625_Lozada http://hdl.handle.net/20.500.12110/paper_14622912_v8_n4_p625_Lozada |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
bacterial RNA ethylene glycol derivative ribosome DNA RNA 16S surfactant terics article bacterium bioreactor classification fluorescence in situ hybridization genetics growth, development and aging instrumentation methodology microbiology phylogeny reverse transcription polymerase chain reaction sewage two dimensional gel electrophoresis water management Bacteria Bioreactors DNA, Ribosomal Electrophoresis, Gel, Two-Dimensional Ethylene Glycols In Situ Hybridization, Fluorescence Phylogeny Reverse Transcriptase Polymerase Chain Reaction RNA, Bacterial RNA, Ribosomal, 16S Sewage Soil Microbiology Surface-Active Agents Water Purification Acidobacteria Alphaproteobacteria Bacteria (microorganisms) Betaproteobacteria Burkholderiales Gammaproteobacteria Sphingomonas |
| spellingShingle |
bacterial RNA ethylene glycol derivative ribosome DNA RNA 16S surfactant terics article bacterium bioreactor classification fluorescence in situ hybridization genetics growth, development and aging instrumentation methodology microbiology phylogeny reverse transcription polymerase chain reaction sewage two dimensional gel electrophoresis water management Bacteria Bioreactors DNA, Ribosomal Electrophoresis, Gel, Two-Dimensional Ethylene Glycols In Situ Hybridization, Fluorescence Phylogeny Reverse Transcriptase Polymerase Chain Reaction RNA, Bacterial RNA, Ribosomal, 16S Sewage Soil Microbiology Surface-Active Agents Water Purification Acidobacteria Alphaproteobacteria Bacteria (microorganisms) Betaproteobacteria Burkholderiales Gammaproteobacteria Sphingomonas Figuerola, Eva Lucía Margarita Itria, Raúl Fabio Erijman, Leonardo Replicability of dominant bacterial populations after long-term surfactant-enrichment in lab-scale activated sludge |
| topic_facet |
bacterial RNA ethylene glycol derivative ribosome DNA RNA 16S surfactant terics article bacterium bioreactor classification fluorescence in situ hybridization genetics growth, development and aging instrumentation methodology microbiology phylogeny reverse transcription polymerase chain reaction sewage two dimensional gel electrophoresis water management Bacteria Bioreactors DNA, Ribosomal Electrophoresis, Gel, Two-Dimensional Ethylene Glycols In Situ Hybridization, Fluorescence Phylogeny Reverse Transcriptase Polymerase Chain Reaction RNA, Bacterial RNA, Ribosomal, 16S Sewage Soil Microbiology Surface-Active Agents Water Purification Acidobacteria Alphaproteobacteria Bacteria (microorganisms) Betaproteobacteria Burkholderiales Gammaproteobacteria Sphingomonas |
| description |
Bacterial communities were examined in replicate lab-scale activated sludge reactors after a period of several months of enrichment with non-ionic nonylphenol ethoxylate (NPE) surfactants. Four sequential batch reactors were fed with synthetic sewage, two of which received additionally NPE. Small subunit rDNA-derived denaturing gel gradient electrophoresis (DGGE) profiles and 16S rDNA clone libraries were dominated by clones of Gammaproteobacteria class. Sequences of the other codominant rDNA phylotypes observed only in DGGE from NPE-amended reactors were, respectively, associated with the Group III of the Acidobacteria phylum. Intriguingly, 16S rRNA content from abundant Gammaproteobacteria cells was unexpectedly low. In addition to Acidobacteria, rRNA-derived DGGE profiles were dominated by members of the order Burkholderiales (of the Betaproteobacteria) and of the genus Sphingomonas (a member of the Alphaproteobacteria). Specific oligonucleotide probes for the selected ribotypes were designed and applied for quantitative real time polymerase chain reaction and fluorescence in situ hybridization, confirming their dominance in treated reactors. The parallel abundance of unique phylotypes in replicate reactors implies a distinctive selection of dominant organisms, which are better adapted to specialized niches in the highly selective environment. © 2005 Society for Applied Microbiology and Blackwell Publishing Ltd. |
| author |
Figuerola, Eva Lucía Margarita Itria, Raúl Fabio Erijman, Leonardo |
| author_facet |
Figuerola, Eva Lucía Margarita Itria, Raúl Fabio Erijman, Leonardo |
| author_sort |
Figuerola, Eva Lucía Margarita |
| title |
Replicability of dominant bacterial populations after long-term surfactant-enrichment in lab-scale activated sludge |
| title_short |
Replicability of dominant bacterial populations after long-term surfactant-enrichment in lab-scale activated sludge |
| title_full |
Replicability of dominant bacterial populations after long-term surfactant-enrichment in lab-scale activated sludge |
| title_fullStr |
Replicability of dominant bacterial populations after long-term surfactant-enrichment in lab-scale activated sludge |
| title_full_unstemmed |
Replicability of dominant bacterial populations after long-term surfactant-enrichment in lab-scale activated sludge |
| title_sort |
replicability of dominant bacterial populations after long-term surfactant-enrichment in lab-scale activated sludge |
| publishDate |
2006 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14622912_v8_n4_p625_Lozada http://hdl.handle.net/20.500.12110/paper_14622912_v8_n4_p625_Lozada |
| work_keys_str_mv |
AT figuerolaevaluciamargarita replicabilityofdominantbacterialpopulationsafterlongtermsurfactantenrichmentinlabscaleactivatedsludge AT itriaraulfabio replicabilityofdominantbacterialpopulationsafterlongtermsurfactantenrichmentinlabscaleactivatedsludge AT erijmanleonardo replicabilityofdominantbacterialpopulationsafterlongtermsurfactantenrichmentinlabscaleactivatedsludge |
| _version_ |
1768542332096872448 |