Effect of copper on diesel degradation in Pseudomonas extremaustralis
Environments co-contaminated with heavy metals and hydrocarbons have become an important problem worldwide, especially due to the effect of metals on hydrocarbon degrading microorganisms. Pseudomonas extremaustralis, a bacterium isolated from a pristine pond in Antarctica, showed high capabilities t...
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2019
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14310651_v23_n1_p91_Colonnella http://hdl.handle.net/20.500.12110/paper_14310651_v23_n1_p91_Colonnella |
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paper:paper_14310651_v23_n1_p91_Colonnella2023-06-08T16:14:06Z Effect of copper on diesel degradation in Pseudomonas extremaustralis AFM Copper resistance Diesel degradation P. extremaustralis Environments co-contaminated with heavy metals and hydrocarbons have become an important problem worldwide, especially due to the effect of metals on hydrocarbon degrading microorganisms. Pseudomonas extremaustralis, a bacterium isolated from a pristine pond in Antarctica, showed high capabilities to cope with environmental stress and a very versatile metabolism that includes alkane degradation under microaerobic conditions. In this work, we analyzed P. extremaustralis’ capability to resist high copper concentrations and the effect of copper presence in diesel biodegradation. We observed that P. extremaustralis resisted up to 4 mM CuSO 4 in a rich medium such as LB. This copper resistance is sustained by the presence of the cus and cop operons together with other efflux systems and porins located in a single region in P. extremaustralis genome. When copper was present, diesel degradation was negatively affected, even though copper enhanced bacterial attachment to hydrocarbons. However, when a small amount of glucose (0.05% w/v) was added, the presence of CuSO 4 enhanced alkane degradation. In addition, atomic force microscopy analysis showed that the presence of glucose decreased the negative effects produced by copper and diesel on the cell envelopes. © 2018, Springer Japan KK, part of Springer Nature. 2019 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14310651_v23_n1_p91_Colonnella http://hdl.handle.net/20.500.12110/paper_14310651_v23_n1_p91_Colonnella |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
AFM Copper resistance Diesel degradation P. extremaustralis |
| spellingShingle |
AFM Copper resistance Diesel degradation P. extremaustralis Effect of copper on diesel degradation in Pseudomonas extremaustralis |
| topic_facet |
AFM Copper resistance Diesel degradation P. extremaustralis |
| description |
Environments co-contaminated with heavy metals and hydrocarbons have become an important problem worldwide, especially due to the effect of metals on hydrocarbon degrading microorganisms. Pseudomonas extremaustralis, a bacterium isolated from a pristine pond in Antarctica, showed high capabilities to cope with environmental stress and a very versatile metabolism that includes alkane degradation under microaerobic conditions. In this work, we analyzed P. extremaustralis’ capability to resist high copper concentrations and the effect of copper presence in diesel biodegradation. We observed that P. extremaustralis resisted up to 4 mM CuSO 4 in a rich medium such as LB. This copper resistance is sustained by the presence of the cus and cop operons together with other efflux systems and porins located in a single region in P. extremaustralis genome. When copper was present, diesel degradation was negatively affected, even though copper enhanced bacterial attachment to hydrocarbons. However, when a small amount of glucose (0.05% w/v) was added, the presence of CuSO 4 enhanced alkane degradation. In addition, atomic force microscopy analysis showed that the presence of glucose decreased the negative effects produced by copper and diesel on the cell envelopes. © 2018, Springer Japan KK, part of Springer Nature. |
| title |
Effect of copper on diesel degradation in Pseudomonas extremaustralis |
| title_short |
Effect of copper on diesel degradation in Pseudomonas extremaustralis |
| title_full |
Effect of copper on diesel degradation in Pseudomonas extremaustralis |
| title_fullStr |
Effect of copper on diesel degradation in Pseudomonas extremaustralis |
| title_full_unstemmed |
Effect of copper on diesel degradation in Pseudomonas extremaustralis |
| title_sort |
effect of copper on diesel degradation in pseudomonas extremaustralis |
| publishDate |
2019 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14310651_v23_n1_p91_Colonnella http://hdl.handle.net/20.500.12110/paper_14310651_v23_n1_p91_Colonnella |
| _version_ |
1768545615197765632 |