Polyhydroxyalkanoates are essential for maintenance of redox state in the Antarctic bacterium Pseudomonas sp. 14-3 during low temperature adaptation
Polyhydroxyalkanoates (PHAs) are highly reduced bacterial storage compounds that increase fitness in changing environments. We have previously shown that phaRBAC genes from the Antarctic bacterium Pseudomonas sp. 14-3 are located in a genomic island containing other genes probably related with its a...
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2009
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14310651_v13_n1_p59_Ayub http://hdl.handle.net/20.500.12110/paper_14310651_v13_n1_p59_Ayub |
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paper:paper_14310651_v13_n1_p59_Ayub2023-06-08T16:14:05Z Polyhydroxyalkanoates are essential for maintenance of redox state in the Antarctic bacterium Pseudomonas sp. 14-3 during low temperature adaptation Ayub, Nicolás Daniel Tribelli, Paula María Antarctica Cold exposure Oxidative stress Polyhydroxyalkanoates Pseudomonas Redox state polyhydroxyalkanoic acid primer DNA adaptation Antarctica article cold genetics growth, development and aging mutation nucleotide sequence oxidation reduction reaction physiology Pseudomonas Adaptation, Physiological Antarctic Regions Base Sequence Cold Temperature DNA Primers Mutation Oxidation-Reduction Polyhydroxyalkanoates Pseudomonas Bacteria (microorganisms) Equus asinus Pseudomonas Pseudomonas sp. 14-3 Polyhydroxyalkanoates (PHAs) are highly reduced bacterial storage compounds that increase fitness in changing environments. We have previously shown that phaRBAC genes from the Antarctic bacterium Pseudomonas sp. 14-3 are located in a genomic island containing other genes probably related with its adaptability to cold environments. In this paper, Pseudomonas sp. 14-3 and its PHA synthase-minus mutant (phaC) were used to asses the effect of PHA accumulation on the adaptability to cold conditions. The phaC mutant was unable to grow at 10°C and was more susceptible to freezing than its parent strain. PHA was necessary for the development of the oxidative stress response induced by cold treatment. Addition of reduced compounds cystine and gluthathione suppressed the cold sensitive phenotype of the phaC mutant. Cold shock produced very rapid degradation of PHA in the wild type strain. The NADH/NAD ratio and NADPH content, estimated by diamide sensitivity, decreased strongly in the mutant after cold shock while only minor changes were observed in the wild type. Accordingly, the level of lipid peroxidation in the mutant strain was 25-fold higher after temperature downshift. We propose that PHA metabolism modulates the availability of reducing equivalents, contributing to alleviate the oxidative stress produced by low temperature. © 2008 Springer. Fil:Ayub, N.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Tribelli, P.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2009 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14310651_v13_n1_p59_Ayub http://hdl.handle.net/20.500.12110/paper_14310651_v13_n1_p59_Ayub |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Antarctica Cold exposure Oxidative stress Polyhydroxyalkanoates Pseudomonas Redox state polyhydroxyalkanoic acid primer DNA adaptation Antarctica article cold genetics growth, development and aging mutation nucleotide sequence oxidation reduction reaction physiology Pseudomonas Adaptation, Physiological Antarctic Regions Base Sequence Cold Temperature DNA Primers Mutation Oxidation-Reduction Polyhydroxyalkanoates Pseudomonas Bacteria (microorganisms) Equus asinus Pseudomonas Pseudomonas sp. 14-3 |
| spellingShingle |
Antarctica Cold exposure Oxidative stress Polyhydroxyalkanoates Pseudomonas Redox state polyhydroxyalkanoic acid primer DNA adaptation Antarctica article cold genetics growth, development and aging mutation nucleotide sequence oxidation reduction reaction physiology Pseudomonas Adaptation, Physiological Antarctic Regions Base Sequence Cold Temperature DNA Primers Mutation Oxidation-Reduction Polyhydroxyalkanoates Pseudomonas Bacteria (microorganisms) Equus asinus Pseudomonas Pseudomonas sp. 14-3 Ayub, Nicolás Daniel Tribelli, Paula María Polyhydroxyalkanoates are essential for maintenance of redox state in the Antarctic bacterium Pseudomonas sp. 14-3 during low temperature adaptation |
| topic_facet |
Antarctica Cold exposure Oxidative stress Polyhydroxyalkanoates Pseudomonas Redox state polyhydroxyalkanoic acid primer DNA adaptation Antarctica article cold genetics growth, development and aging mutation nucleotide sequence oxidation reduction reaction physiology Pseudomonas Adaptation, Physiological Antarctic Regions Base Sequence Cold Temperature DNA Primers Mutation Oxidation-Reduction Polyhydroxyalkanoates Pseudomonas Bacteria (microorganisms) Equus asinus Pseudomonas Pseudomonas sp. 14-3 |
| description |
Polyhydroxyalkanoates (PHAs) are highly reduced bacterial storage compounds that increase fitness in changing environments. We have previously shown that phaRBAC genes from the Antarctic bacterium Pseudomonas sp. 14-3 are located in a genomic island containing other genes probably related with its adaptability to cold environments. In this paper, Pseudomonas sp. 14-3 and its PHA synthase-minus mutant (phaC) were used to asses the effect of PHA accumulation on the adaptability to cold conditions. The phaC mutant was unable to grow at 10°C and was more susceptible to freezing than its parent strain. PHA was necessary for the development of the oxidative stress response induced by cold treatment. Addition of reduced compounds cystine and gluthathione suppressed the cold sensitive phenotype of the phaC mutant. Cold shock produced very rapid degradation of PHA in the wild type strain. The NADH/NAD ratio and NADPH content, estimated by diamide sensitivity, decreased strongly in the mutant after cold shock while only minor changes were observed in the wild type. Accordingly, the level of lipid peroxidation in the mutant strain was 25-fold higher after temperature downshift. We propose that PHA metabolism modulates the availability of reducing equivalents, contributing to alleviate the oxidative stress produced by low temperature. © 2008 Springer. |
| author |
Ayub, Nicolás Daniel Tribelli, Paula María |
| author_facet |
Ayub, Nicolás Daniel Tribelli, Paula María |
| author_sort |
Ayub, Nicolás Daniel |
| title |
Polyhydroxyalkanoates are essential for maintenance of redox state in the Antarctic bacterium Pseudomonas sp. 14-3 during low temperature adaptation |
| title_short |
Polyhydroxyalkanoates are essential for maintenance of redox state in the Antarctic bacterium Pseudomonas sp. 14-3 during low temperature adaptation |
| title_full |
Polyhydroxyalkanoates are essential for maintenance of redox state in the Antarctic bacterium Pseudomonas sp. 14-3 during low temperature adaptation |
| title_fullStr |
Polyhydroxyalkanoates are essential for maintenance of redox state in the Antarctic bacterium Pseudomonas sp. 14-3 during low temperature adaptation |
| title_full_unstemmed |
Polyhydroxyalkanoates are essential for maintenance of redox state in the Antarctic bacterium Pseudomonas sp. 14-3 during low temperature adaptation |
| title_sort |
polyhydroxyalkanoates are essential for maintenance of redox state in the antarctic bacterium pseudomonas sp. 14-3 during low temperature adaptation |
| publishDate |
2009 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14310651_v13_n1_p59_Ayub http://hdl.handle.net/20.500.12110/paper_14310651_v13_n1_p59_Ayub |
| work_keys_str_mv |
AT ayubnicolasdaniel polyhydroxyalkanoatesareessentialformaintenanceofredoxstateintheantarcticbacteriumpseudomonassp143duringlowtemperatureadaptation AT tribellipaulamaria polyhydroxyalkanoatesareessentialformaintenanceofredoxstateintheantarcticbacteriumpseudomonassp143duringlowtemperatureadaptation |
| _version_ |
1768546737911234560 |