Development of a novel method for in vivo determination of activation energy of glucose transport across S. cerevisiae cellular membranes. A biosensor-like approach

Whereas biosensors have been usually proposed as analytical tools, used to investigate the surrounding media pursuing an analytical answer, we have used a biosensor-like device to characterize the microbial cells immobilized on it. We have studied the kinetics of transport and degradation of glucose...

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Autor principal:	Cortón, Eduardo
Publicado:	2009
Materias:	Activation energy Arrhenius plot Biosensors Glucose transport Microbial metabolism Saccharomyces cerevisiae Analytical tool Cellular membranes Glucose concentration In-vivo Microbial cells S.cerevisiae Arrhenius plots Cell membranes Glucose Metabolism Yeast
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14248220_v9_n3_p1599_Kormes http://hdl.handle.net/20.500.12110/paper_14248220_v9_n3_p1599_Kormes
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_14248220_v9_n3_p1599_Kormes
record_format	dspace
spelling	paper:paper_14248220_v9_n3_p1599_Kormes2023-06-08T16:13:59Z Development of a novel method for in vivo determination of activation energy of glucose transport across S. cerevisiae cellular membranes. A biosensor-like approach Cortón, Eduardo Activation energy Arrhenius plot Biosensors Glucose transport Microbial metabolism Saccharomyces cerevisiae Analytical tool Cellular membranes Glucose concentration Glucose transport In-vivo Microbial cells Microbial metabolism S.cerevisiae Activation energy Arrhenius plots Cell membranes Glucose Metabolism Yeast Biosensors Whereas biosensors have been usually proposed as analytical tools, used to investigate the surrounding media pursuing an analytical answer, we have used a biosensor-like device to characterize the microbial cells immobilized on it. We have studied the kinetics of transport and degradation of glucose at different concentrations and temperatures. When glucose concentrations of 15 and 1.5 mM were assayed, calculated activation energies were 25.2 and 18.4 kcal mol-1, respectively, in good agreement with previously published data. The opportunity and convenience of using Arrhenius plots to estimate the activation energy in metabolic-related processes is also discussed. © 2009 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. Fil:Cortón, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2009 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14248220_v9_n3_p1599_Kormes http://hdl.handle.net/20.500.12110/paper_14248220_v9_n3_p1599_Kormes
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Activation energy Arrhenius plot Biosensors Glucose transport Microbial metabolism Saccharomyces cerevisiae Analytical tool Cellular membranes Glucose concentration Glucose transport In-vivo Microbial cells Microbial metabolism S.cerevisiae Activation energy Arrhenius plots Cell membranes Glucose Metabolism Yeast Biosensors
spellingShingle	Activation energy Arrhenius plot Biosensors Glucose transport Microbial metabolism Saccharomyces cerevisiae Analytical tool Cellular membranes Glucose concentration Glucose transport In-vivo Microbial cells Microbial metabolism S.cerevisiae Activation energy Arrhenius plots Cell membranes Glucose Metabolism Yeast Biosensors Cortón, Eduardo Development of a novel method for in vivo determination of activation energy of glucose transport across S. cerevisiae cellular membranes. A biosensor-like approach
topic_facet	Activation energy Arrhenius plot Biosensors Glucose transport Microbial metabolism Saccharomyces cerevisiae Analytical tool Cellular membranes Glucose concentration Glucose transport In-vivo Microbial cells Microbial metabolism S.cerevisiae Activation energy Arrhenius plots Cell membranes Glucose Metabolism Yeast Biosensors
description	Whereas biosensors have been usually proposed as analytical tools, used to investigate the surrounding media pursuing an analytical answer, we have used a biosensor-like device to characterize the microbial cells immobilized on it. We have studied the kinetics of transport and degradation of glucose at different concentrations and temperatures. When glucose concentrations of 15 and 1.5 mM were assayed, calculated activation energies were 25.2 and 18.4 kcal mol-1, respectively, in good agreement with previously published data. The opportunity and convenience of using Arrhenius plots to estimate the activation energy in metabolic-related processes is also discussed. © 2009 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.
author	Cortón, Eduardo
author_facet	Cortón, Eduardo
author_sort	Cortón, Eduardo
title	Development of a novel method for in vivo determination of activation energy of glucose transport across S. cerevisiae cellular membranes. A biosensor-like approach
title_short	Development of a novel method for in vivo determination of activation energy of glucose transport across S. cerevisiae cellular membranes. A biosensor-like approach
title_full	Development of a novel method for in vivo determination of activation energy of glucose transport across S. cerevisiae cellular membranes. A biosensor-like approach
title_fullStr	Development of a novel method for in vivo determination of activation energy of glucose transport across S. cerevisiae cellular membranes. A biosensor-like approach
title_full_unstemmed	Development of a novel method for in vivo determination of activation energy of glucose transport across S. cerevisiae cellular membranes. A biosensor-like approach
title_sort	development of a novel method for in vivo determination of activation energy of glucose transport across s. cerevisiae cellular membranes. a biosensor-like approach
publishDate	2009
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14248220_v9_n3_p1599_Kormes http://hdl.handle.net/20.500.12110/paper_14248220_v9_n3_p1599_Kormes
work_keys_str_mv	AT cortoneduardo developmentofanovelmethodforinvivodeterminationofactivationenergyofglucosetransportacrossscerevisiaecellularmembranesabiosensorlikeapproach
_version_	1768545204212596736

Development of a novel method for in vivo determination of activation energy of glucose transport across S. cerevisiae cellular membranes. A biosensor-like approach

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