Development of a biosensor for environmental monitoring based on microalgae immobilized in silica hydrogels
A new biosensor was designed for the assessment of aquatic environment quality. Three microalgae were used as toxicity bioindicators: Chlorella vulgaris, Pseudokirchneriella subcapitata and Chlamydomonas reinhardtii. These microalgae were immobilized in alginate and silica hydrogels in a two step pr...
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2012
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14248220_v12_n12_p16879_Ferro http://hdl.handle.net/20.500.12110/paper_14248220_v12_n12_p16879_Ferro |
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paper:paper_14248220_v12_n12_p16879_Ferro2023-06-08T16:13:58Z Development of a biosensor for environmental monitoring based on microalgae immobilized in silica hydrogels Perullini, Ana Mercedes Jobbagy, Matias Aldabe Bilmes, Sara Alfonsina Dora Algae Atrazine Biosensor Chlorophyll fluorescence DCMU Herbicides Sol-gel Aquatic environments Chlamydomonas reinhardtii Chlorophyll fluorescence DCMU Environmental Monitoring Fluorescence enhancement Pseudokirchneriella subcapitata Two-step procedure Algae Herbicides Hydrogels Microorganisms Silica Sol-gels Weed control Biosensors atrazine diuron fresh water article chemistry Chlamydomonas reinhardtii Chlorella vulgaris environmental monitoring equipment genetic procedures human hydrogel isolation and purification microalga water pollutant Atrazine Biosensing Techniques Chlamydomonas reinhardtii Chlorella vulgaris Diuron Environmental Monitoring Fresh Water Humans Hydrogel Microalgae Water Pollutants, Chemical A new biosensor was designed for the assessment of aquatic environment quality. Three microalgae were used as toxicity bioindicators: Chlorella vulgaris, Pseudokirchneriella subcapitata and Chlamydomonas reinhardtii. These microalgae were immobilized in alginate and silica hydrogels in a two step procedure. After studying the growth rate of entrapped cells, chlorophyll fluorescence was measured after exposure to (3-(3,4-dichlorophenyl)-1,1-dimethylurea) (DCMU) and various concentrations of the common herbicide atrazine. Microalgae are very sensitive to herbicides and detection of fluorescence enhancement with very good efficiency was realized. The best detection limit was 0.1 μM, obtained with the strain C. reinhardtii after 40 minutes of exposure. © 2012 by the authors; licensee MDPI, Basel, Switzerland. Fil:Perullini, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Jobbagy, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Bilmes, S.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2012 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14248220_v12_n12_p16879_Ferro http://hdl.handle.net/20.500.12110/paper_14248220_v12_n12_p16879_Ferro |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Algae Atrazine Biosensor Chlorophyll fluorescence DCMU Herbicides Sol-gel Aquatic environments Chlamydomonas reinhardtii Chlorophyll fluorescence DCMU Environmental Monitoring Fluorescence enhancement Pseudokirchneriella subcapitata Two-step procedure Algae Herbicides Hydrogels Microorganisms Silica Sol-gels Weed control Biosensors atrazine diuron fresh water article chemistry Chlamydomonas reinhardtii Chlorella vulgaris environmental monitoring equipment genetic procedures human hydrogel isolation and purification microalga water pollutant Atrazine Biosensing Techniques Chlamydomonas reinhardtii Chlorella vulgaris Diuron Environmental Monitoring Fresh Water Humans Hydrogel Microalgae Water Pollutants, Chemical |
| spellingShingle |
Algae Atrazine Biosensor Chlorophyll fluorescence DCMU Herbicides Sol-gel Aquatic environments Chlamydomonas reinhardtii Chlorophyll fluorescence DCMU Environmental Monitoring Fluorescence enhancement Pseudokirchneriella subcapitata Two-step procedure Algae Herbicides Hydrogels Microorganisms Silica Sol-gels Weed control Biosensors atrazine diuron fresh water article chemistry Chlamydomonas reinhardtii Chlorella vulgaris environmental monitoring equipment genetic procedures human hydrogel isolation and purification microalga water pollutant Atrazine Biosensing Techniques Chlamydomonas reinhardtii Chlorella vulgaris Diuron Environmental Monitoring Fresh Water Humans Hydrogel Microalgae Water Pollutants, Chemical Perullini, Ana Mercedes Jobbagy, Matias Aldabe Bilmes, Sara Alfonsina Dora Development of a biosensor for environmental monitoring based on microalgae immobilized in silica hydrogels |
| topic_facet |
Algae Atrazine Biosensor Chlorophyll fluorescence DCMU Herbicides Sol-gel Aquatic environments Chlamydomonas reinhardtii Chlorophyll fluorescence DCMU Environmental Monitoring Fluorescence enhancement Pseudokirchneriella subcapitata Two-step procedure Algae Herbicides Hydrogels Microorganisms Silica Sol-gels Weed control Biosensors atrazine diuron fresh water article chemistry Chlamydomonas reinhardtii Chlorella vulgaris environmental monitoring equipment genetic procedures human hydrogel isolation and purification microalga water pollutant Atrazine Biosensing Techniques Chlamydomonas reinhardtii Chlorella vulgaris Diuron Environmental Monitoring Fresh Water Humans Hydrogel Microalgae Water Pollutants, Chemical |
| description |
A new biosensor was designed for the assessment of aquatic environment quality. Three microalgae were used as toxicity bioindicators: Chlorella vulgaris, Pseudokirchneriella subcapitata and Chlamydomonas reinhardtii. These microalgae were immobilized in alginate and silica hydrogels in a two step procedure. After studying the growth rate of entrapped cells, chlorophyll fluorescence was measured after exposure to (3-(3,4-dichlorophenyl)-1,1-dimethylurea) (DCMU) and various concentrations of the common herbicide atrazine. Microalgae are very sensitive to herbicides and detection of fluorescence enhancement with very good efficiency was realized. The best detection limit was 0.1 μM, obtained with the strain C. reinhardtii after 40 minutes of exposure. © 2012 by the authors; licensee MDPI, Basel, Switzerland. |
| author |
Perullini, Ana Mercedes Jobbagy, Matias Aldabe Bilmes, Sara Alfonsina Dora |
| author_facet |
Perullini, Ana Mercedes Jobbagy, Matias Aldabe Bilmes, Sara Alfonsina Dora |
| author_sort |
Perullini, Ana Mercedes |
| title |
Development of a biosensor for environmental monitoring based on microalgae immobilized in silica hydrogels |
| title_short |
Development of a biosensor for environmental monitoring based on microalgae immobilized in silica hydrogels |
| title_full |
Development of a biosensor for environmental monitoring based on microalgae immobilized in silica hydrogels |
| title_fullStr |
Development of a biosensor for environmental monitoring based on microalgae immobilized in silica hydrogels |
| title_full_unstemmed |
Development of a biosensor for environmental monitoring based on microalgae immobilized in silica hydrogels |
| title_sort |
development of a biosensor for environmental monitoring based on microalgae immobilized in silica hydrogels |
| publishDate |
2012 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14248220_v12_n12_p16879_Ferro http://hdl.handle.net/20.500.12110/paper_14248220_v12_n12_p16879_Ferro |
| work_keys_str_mv |
AT perullinianamercedes developmentofabiosensorforenvironmentalmonitoringbasedonmicroalgaeimmobilizedinsilicahydrogels AT jobbagymatias developmentofabiosensorforenvironmentalmonitoringbasedonmicroalgaeimmobilizedinsilicahydrogels AT aldabebilmessaraalfonsinadora developmentofabiosensorforenvironmentalmonitoringbasedonmicroalgaeimmobilizedinsilicahydrogels |
| _version_ |
1768544610833924096 |