Autohydrolysis of a partially cyclized mu/nu-carrageenan and structural elucidation of the oligosaccharides by chemical analysis, NMR spectroscopy and UV-MALDI mass spectrometry

A partially cyclized mu/nu-carrageenan from Gigartina skottsbergii was submitted to autohydrolysis and the resulting mixture of oligosaccharides was fractionated using sequential dialysis through tubings of molecular weight cutoff 12000, 3500 and 1000. In this way four fractions were separated and c...

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Detalles Bibliográficos
Publicado: 2005
Materias:
Autohydrolysis
Carrageenans
Nor-harmane
Structural analysis
Sulfated galactans
Sulfated oligosaccharides
UV-MALDI-TOF-MS
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14246376_v2005_n12_p319_Ciancia
http://hdl.handle.net/20.500.12110/paper_14246376_v2005_n12_p319_Ciancia
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_14246376_v2005_n12_p319_Ciancia
record_format dspace
spelling paper:paper_14246376_v2005_n12_p319_Ciancia2023-06-08T16:13:57Z Autohydrolysis of a partially cyclized mu/nu-carrageenan and structural elucidation of the oligosaccharides by chemical analysis, NMR spectroscopy and UV-MALDI mass spectrometry Autohydrolysis Carrageenans Nor-harmane Structural analysis Sulfated galactans Sulfated oligosaccharides UV-MALDI-TOF-MS A partially cyclized mu/nu-carrageenan from Gigartina skottsbergii was submitted to autohydrolysis and the resulting mixture of oligosaccharides was fractionated using sequential dialysis through tubings of molecular weight cutoff 12000, 3500 and 1000. In this way four fractions were separated and characterized. Structural analysis of the major fraction, obtained from the solution inside the dialysis tubing of molecular weight cutoff 12000 (D120), was carried out. A significant difference was found by 13C NMR spectroscopy and UV-MALDI-TOF mass spectrometry: in the 13C NMR spectrum the reducing end-chain unit was the hydrated aldehyde of 3,6-anhydrogalactose while the UV-MALDI-TOF mass spectrum showed an unimodal distribution of even and odd peaks, suggesting fragmentation of glycosidic linkages. © ARKAT. 2005 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14246376_v2005_n12_p319_Ciancia http://hdl.handle.net/20.500.12110/paper_14246376_v2005_n12_p319_Ciancia
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Autohydrolysis
Carrageenans
Nor-harmane
Structural analysis
Sulfated galactans
Sulfated oligosaccharides
UV-MALDI-TOF-MS
spellingShingle Autohydrolysis
Carrageenans
Nor-harmane
Structural analysis
Sulfated galactans
Sulfated oligosaccharides
UV-MALDI-TOF-MS
Autohydrolysis of a partially cyclized mu/nu-carrageenan and structural elucidation of the oligosaccharides by chemical analysis, NMR spectroscopy and UV-MALDI mass spectrometry
topic_facet Autohydrolysis
Carrageenans
Nor-harmane
Structural analysis
Sulfated galactans
Sulfated oligosaccharides
UV-MALDI-TOF-MS
description A partially cyclized mu/nu-carrageenan from Gigartina skottsbergii was submitted to autohydrolysis and the resulting mixture of oligosaccharides was fractionated using sequential dialysis through tubings of molecular weight cutoff 12000, 3500 and 1000. In this way four fractions were separated and characterized. Structural analysis of the major fraction, obtained from the solution inside the dialysis tubing of molecular weight cutoff 12000 (D120), was carried out. A significant difference was found by 13C NMR spectroscopy and UV-MALDI-TOF mass spectrometry: in the 13C NMR spectrum the reducing end-chain unit was the hydrated aldehyde of 3,6-anhydrogalactose while the UV-MALDI-TOF mass spectrum showed an unimodal distribution of even and odd peaks, suggesting fragmentation of glycosidic linkages. © ARKAT.
title Autohydrolysis of a partially cyclized mu/nu-carrageenan and structural elucidation of the oligosaccharides by chemical analysis, NMR spectroscopy and UV-MALDI mass spectrometry
title_short Autohydrolysis of a partially cyclized mu/nu-carrageenan and structural elucidation of the oligosaccharides by chemical analysis, NMR spectroscopy and UV-MALDI mass spectrometry
title_full Autohydrolysis of a partially cyclized mu/nu-carrageenan and structural elucidation of the oligosaccharides by chemical analysis, NMR spectroscopy and UV-MALDI mass spectrometry
title_fullStr Autohydrolysis of a partially cyclized mu/nu-carrageenan and structural elucidation of the oligosaccharides by chemical analysis, NMR spectroscopy and UV-MALDI mass spectrometry
title_full_unstemmed Autohydrolysis of a partially cyclized mu/nu-carrageenan and structural elucidation of the oligosaccharides by chemical analysis, NMR spectroscopy and UV-MALDI mass spectrometry
title_sort autohydrolysis of a partially cyclized mu/nu-carrageenan and structural elucidation of the oligosaccharides by chemical analysis, nmr spectroscopy and uv-maldi mass spectrometry
publishDate 2005
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14246376_v2005_n12_p319_Ciancia
http://hdl.handle.net/20.500.12110/paper_14246376_v2005_n12_p319_Ciancia
_version_ 1768545524781154304

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