Comparison of micro-enzymatic and high performance anion exchange chromatography methods for the analysis of glucose in human serum

In this study we have performed a comparison of the most used spectroscopic glucose-oxidase assay for routine clinical monitoring of serum glucose, with the high-performance anion-exchange chromatographic method using pulsed amperometric detection (HPAEC-PAD). The linearity, repeatability and intern...

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Detalles Bibliográficos
Publicado: 2006
Materias:
Glucose determination
Glucose-oxidase method
HPAEC-PAD
Human serum analysis
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14246376_v2005_n12_p243_Casabuono
http://hdl.handle.net/20.500.12110/paper_14246376_v2005_n12_p243_Casabuono
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_14246376_v2005_n12_p243_Casabuono
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spelling paper:paper_14246376_v2005_n12_p243_Casabuono2023-06-08T16:13:56Z Comparison of micro-enzymatic and high performance anion exchange chromatography methods for the analysis of glucose in human serum Glucose determination Glucose-oxidase method HPAEC-PAD Human serum analysis In this study we have performed a comparison of the most used spectroscopic glucose-oxidase assay for routine clinical monitoring of serum glucose, with the high-performance anion-exchange chromatographic method using pulsed amperometric detection (HPAEC-PAD). The linearity, repeatability and internal reproducibility of the chromatographic method were determined. The correlation by linear regression and difference plot was analyzed. It is known that the enzymatic method leads to unreliable results when glucose must be determined in samples containing high concentration of bilirubin or lipids. On the contrary, the accuracy of the HPAEC-PAD method with these complex matrix sera resulted similar to normal samples showing no interferences. Another advantage was the minimal amount of human serum needed: one microliter was largely sufficient to perform the whole analysis. This fact would make the HPAEC-PAD method applicable when samples of preterm infants or new born babies of low weight must be analyzed. ©ARKAT. 2006 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14246376_v2005_n12_p243_Casabuono http://hdl.handle.net/20.500.12110/paper_14246376_v2005_n12_p243_Casabuono
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Glucose determination
Glucose-oxidase method
HPAEC-PAD
Human serum analysis
spellingShingle Glucose determination
Glucose-oxidase method
HPAEC-PAD
Human serum analysis
Comparison of micro-enzymatic and high performance anion exchange chromatography methods for the analysis of glucose in human serum
topic_facet Glucose determination
Glucose-oxidase method
HPAEC-PAD
Human serum analysis
description In this study we have performed a comparison of the most used spectroscopic glucose-oxidase assay for routine clinical monitoring of serum glucose, with the high-performance anion-exchange chromatographic method using pulsed amperometric detection (HPAEC-PAD). The linearity, repeatability and internal reproducibility of the chromatographic method were determined. The correlation by linear regression and difference plot was analyzed. It is known that the enzymatic method leads to unreliable results when glucose must be determined in samples containing high concentration of bilirubin or lipids. On the contrary, the accuracy of the HPAEC-PAD method with these complex matrix sera resulted similar to normal samples showing no interferences. Another advantage was the minimal amount of human serum needed: one microliter was largely sufficient to perform the whole analysis. This fact would make the HPAEC-PAD method applicable when samples of preterm infants or new born babies of low weight must be analyzed. ©ARKAT.
title Comparison of micro-enzymatic and high performance anion exchange chromatography methods for the analysis of glucose in human serum
title_short Comparison of micro-enzymatic and high performance anion exchange chromatography methods for the analysis of glucose in human serum
title_full Comparison of micro-enzymatic and high performance anion exchange chromatography methods for the analysis of glucose in human serum
title_fullStr Comparison of micro-enzymatic and high performance anion exchange chromatography methods for the analysis of glucose in human serum
title_full_unstemmed Comparison of micro-enzymatic and high performance anion exchange chromatography methods for the analysis of glucose in human serum
title_sort comparison of micro-enzymatic and high performance anion exchange chromatography methods for the analysis of glucose in human serum
publishDate 2006
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14246376_v2005_n12_p243_Casabuono
http://hdl.handle.net/20.500.12110/paper_14246376_v2005_n12_p243_Casabuono
_version_ 1768543528413036544

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