Isozyme and RAPD studies in Prosopis glandulosa and P. velutina (Leguminosae, Mimosoideae)
Allozyme and random amplified polymorphic DNA (RAPD) techniques have been compared for their usefulness for genetic and taxonomic studies in Prosopis glandulosa and P. velutina populations. Isozymes and RAPDs yielded similarly high estimates of genetic variability. Genetic structure and differentiat...
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2000
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paper:paper_14154757_v23_n3_p639_Bessega2023-06-08T16:13:28Z Isozyme and RAPD studies in Prosopis glandulosa and P. velutina (Leguminosae, Mimosoideae) Bessega, Cecilia Fabiana Saidman, Beatriz Ofelia Vilardi, Juan Cesar alcohol dehydrogenase aminopeptidase aspartate aminotransferase esterase isocitrate dehydrogenase isoenzyme peroxidase plant DNA superoxide dismutase article climate controlled study correlation function data analysis diagnostic value gene frequency gene structure gene transfer genetic analysis genetic variability geographic distribution inheritance legume marker gene nonhuman random amplified polymorphic DNA species difference taxonomy Prosopis glandulosa Prosopis velutina Allozyme and random amplified polymorphic DNA (RAPD) techniques have been compared for their usefulness for genetic and taxonomic studies in Prosopis glandulosa and P. velutina populations. Isozymes and RAPDs yielded similarly high estimates of genetic variability. Genetic structure and differentiation were analyzed through non-hierarchical Wright's FDT. For all populations considered, both markers produced low gene flow (Nm < 1) estimates. When only P. glandulosa populations were analyzed, isozyme data yielded higher gene flow estimates (Nm > 1), in agreement with that expected for conspecific populations. However, in RAPD data the expected reduction in FDT and the increase in Nm were not observed. Correlation between FDT and geographical distance matrices (Mantel test) for all populations was significant (P = 0.02) when based on isozymes, but not so (P = 0.33) when based on RAPDs. No significant associations among genetic and geographical or climatic variables were observed. Two isoenzyme systems (GOT and PRX) enabled us to distinguish between P. glandulosa and P. velutina, but no diagnostic band for recognition of populations or species studied here were detected by RAPD. However, RAPD markers showed higher values for genetic differentiation among conspecific populations of P. glandulosa and a lower coefficient of variation than those obtained from isozymes. Fil:Bessega, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Saidman, B.O. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Vilardi, J.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2000 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14154757_v23_n3_p639_Bessega http://hdl.handle.net/20.500.12110/paper_14154757_v23_n3_p639_Bessega |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
alcohol dehydrogenase aminopeptidase aspartate aminotransferase esterase isocitrate dehydrogenase isoenzyme peroxidase plant DNA superoxide dismutase article climate controlled study correlation function data analysis diagnostic value gene frequency gene structure gene transfer genetic analysis genetic variability geographic distribution inheritance legume marker gene nonhuman random amplified polymorphic DNA species difference taxonomy Prosopis glandulosa Prosopis velutina |
| spellingShingle |
alcohol dehydrogenase aminopeptidase aspartate aminotransferase esterase isocitrate dehydrogenase isoenzyme peroxidase plant DNA superoxide dismutase article climate controlled study correlation function data analysis diagnostic value gene frequency gene structure gene transfer genetic analysis genetic variability geographic distribution inheritance legume marker gene nonhuman random amplified polymorphic DNA species difference taxonomy Prosopis glandulosa Prosopis velutina Bessega, Cecilia Fabiana Saidman, Beatriz Ofelia Vilardi, Juan Cesar Isozyme and RAPD studies in Prosopis glandulosa and P. velutina (Leguminosae, Mimosoideae) |
| topic_facet |
alcohol dehydrogenase aminopeptidase aspartate aminotransferase esterase isocitrate dehydrogenase isoenzyme peroxidase plant DNA superoxide dismutase article climate controlled study correlation function data analysis diagnostic value gene frequency gene structure gene transfer genetic analysis genetic variability geographic distribution inheritance legume marker gene nonhuman random amplified polymorphic DNA species difference taxonomy Prosopis glandulosa Prosopis velutina |
| description |
Allozyme and random amplified polymorphic DNA (RAPD) techniques have been compared for their usefulness for genetic and taxonomic studies in Prosopis glandulosa and P. velutina populations. Isozymes and RAPDs yielded similarly high estimates of genetic variability. Genetic structure and differentiation were analyzed through non-hierarchical Wright's FDT. For all populations considered, both markers produced low gene flow (Nm < 1) estimates. When only P. glandulosa populations were analyzed, isozyme data yielded higher gene flow estimates (Nm > 1), in agreement with that expected for conspecific populations. However, in RAPD data the expected reduction in FDT and the increase in Nm were not observed. Correlation between FDT and geographical distance matrices (Mantel test) for all populations was significant (P = 0.02) when based on isozymes, but not so (P = 0.33) when based on RAPDs. No significant associations among genetic and geographical or climatic variables were observed. Two isoenzyme systems (GOT and PRX) enabled us to distinguish between P. glandulosa and P. velutina, but no diagnostic band for recognition of populations or species studied here were detected by RAPD. However, RAPD markers showed higher values for genetic differentiation among conspecific populations of P. glandulosa and a lower coefficient of variation than those obtained from isozymes. |
| author |
Bessega, Cecilia Fabiana Saidman, Beatriz Ofelia Vilardi, Juan Cesar |
| author_facet |
Bessega, Cecilia Fabiana Saidman, Beatriz Ofelia Vilardi, Juan Cesar |
| author_sort |
Bessega, Cecilia Fabiana |
| title |
Isozyme and RAPD studies in Prosopis glandulosa and P. velutina (Leguminosae, Mimosoideae) |
| title_short |
Isozyme and RAPD studies in Prosopis glandulosa and P. velutina (Leguminosae, Mimosoideae) |
| title_full |
Isozyme and RAPD studies in Prosopis glandulosa and P. velutina (Leguminosae, Mimosoideae) |
| title_fullStr |
Isozyme and RAPD studies in Prosopis glandulosa and P. velutina (Leguminosae, Mimosoideae) |
| title_full_unstemmed |
Isozyme and RAPD studies in Prosopis glandulosa and P. velutina (Leguminosae, Mimosoideae) |
| title_sort |
isozyme and rapd studies in prosopis glandulosa and p. velutina (leguminosae, mimosoideae) |
| publishDate |
2000 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14154757_v23_n3_p639_Bessega http://hdl.handle.net/20.500.12110/paper_14154757_v23_n3_p639_Bessega |
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AT bessegaceciliafabiana isozymeandrapdstudiesinprosopisglandulosaandpvelutinaleguminosaemimosoideae AT saidmanbeatrizofelia isozymeandrapdstudiesinprosopisglandulosaandpvelutinaleguminosaemimosoideae AT vilardijuancesar isozymeandrapdstudiesinprosopisglandulosaandpvelutinaleguminosaemimosoideae |
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