Latest advances in OBOC peptide libraries. Improvements in screening strategies and enlarging the family from linear to cyclic libraries
Solid phase screenings of one bead one compound (OBOC) libraries have been widely used to find ligands with pharmacological and analytical uses, and to purify or detect proteins in complex mixtures. To improve library screening, in the last years various strategies have been developed to avoid the s...
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2016
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13892010_v17_n5_p449_MartinezCeron http://hdl.handle.net/20.500.12110/paper_13892010_v17_n5_p449_MartinezCeron |
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paper:paper_13892010_v17_n5_p449_MartinezCeron2023-06-08T16:13:08Z Latest advances in OBOC peptide libraries. Improvements in screening strategies and enlarging the family from linear to cyclic libraries Affinity Cyclic Libraries Mass spectrometry Peptide Peptoid Tandem MS/MS amino acid trifluoroacetic acid cyclopeptide ligand peptide library Article combinatorial library drug selectivity electrospray enzymatic degradation human mass spectrometry matrix assisted laser desorption ionization time of flight mass spectrometry one bead one compound peptide library peptide library screening tandem mass spectrometry chemistry Humans Ligands Peptide Library Peptides, Cyclic Tandem Mass Spectrometry Solid phase screenings of one bead one compound (OBOC) libraries have been widely used to find ligands with pharmacological and analytical uses, and to purify or detect proteins in complex mixtures. To improve library screening, in the last years various strategies have been developed to avoid the selection of false positive beads and to obtain selective ligands. Currently, there is great interest in cyclic peptides because of their resistance to enzymatic degradation and higher selectivity compared to their linear counterparts. Lots of cyclic peptide libraries protocols have been recently developed to facilitate hits analysis. The aim of this review is to summarize the latest applications of solid phase screening of OBOC combinatorial peptide libraries, the improvements in the screening methods including mass spectrometry MS/MS techniques and the strategies to synthesize OBOC cyclic peptide libraries. © 2016 Bentham Science Publishers. 2016 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13892010_v17_n5_p449_MartinezCeron http://hdl.handle.net/20.500.12110/paper_13892010_v17_n5_p449_MartinezCeron |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Affinity Cyclic Libraries Mass spectrometry Peptide Peptoid Tandem MS/MS amino acid trifluoroacetic acid cyclopeptide ligand peptide library Article combinatorial library drug selectivity electrospray enzymatic degradation human mass spectrometry matrix assisted laser desorption ionization time of flight mass spectrometry one bead one compound peptide library peptide library screening tandem mass spectrometry chemistry Humans Ligands Peptide Library Peptides, Cyclic Tandem Mass Spectrometry |
| spellingShingle |
Affinity Cyclic Libraries Mass spectrometry Peptide Peptoid Tandem MS/MS amino acid trifluoroacetic acid cyclopeptide ligand peptide library Article combinatorial library drug selectivity electrospray enzymatic degradation human mass spectrometry matrix assisted laser desorption ionization time of flight mass spectrometry one bead one compound peptide library peptide library screening tandem mass spectrometry chemistry Humans Ligands Peptide Library Peptides, Cyclic Tandem Mass Spectrometry Latest advances in OBOC peptide libraries. Improvements in screening strategies and enlarging the family from linear to cyclic libraries |
| topic_facet |
Affinity Cyclic Libraries Mass spectrometry Peptide Peptoid Tandem MS/MS amino acid trifluoroacetic acid cyclopeptide ligand peptide library Article combinatorial library drug selectivity electrospray enzymatic degradation human mass spectrometry matrix assisted laser desorption ionization time of flight mass spectrometry one bead one compound peptide library peptide library screening tandem mass spectrometry chemistry Humans Ligands Peptide Library Peptides, Cyclic Tandem Mass Spectrometry |
| description |
Solid phase screenings of one bead one compound (OBOC) libraries have been widely used to find ligands with pharmacological and analytical uses, and to purify or detect proteins in complex mixtures. To improve library screening, in the last years various strategies have been developed to avoid the selection of false positive beads and to obtain selective ligands. Currently, there is great interest in cyclic peptides because of their resistance to enzymatic degradation and higher selectivity compared to their linear counterparts. Lots of cyclic peptide libraries protocols have been recently developed to facilitate hits analysis. The aim of this review is to summarize the latest applications of solid phase screening of OBOC combinatorial peptide libraries, the improvements in the screening methods including mass spectrometry MS/MS techniques and the strategies to synthesize OBOC cyclic peptide libraries. © 2016 Bentham Science Publishers. |
| title |
Latest advances in OBOC peptide libraries. Improvements in screening strategies and enlarging the family from linear to cyclic libraries |
| title_short |
Latest advances in OBOC peptide libraries. Improvements in screening strategies and enlarging the family from linear to cyclic libraries |
| title_full |
Latest advances in OBOC peptide libraries. Improvements in screening strategies and enlarging the family from linear to cyclic libraries |
| title_fullStr |
Latest advances in OBOC peptide libraries. Improvements in screening strategies and enlarging the family from linear to cyclic libraries |
| title_full_unstemmed |
Latest advances in OBOC peptide libraries. Improvements in screening strategies and enlarging the family from linear to cyclic libraries |
| title_sort |
latest advances in oboc peptide libraries. improvements in screening strategies and enlarging the family from linear to cyclic libraries |
| publishDate |
2016 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13892010_v17_n5_p449_MartinezCeron http://hdl.handle.net/20.500.12110/paper_13892010_v17_n5_p449_MartinezCeron |
| _version_ |
1768542520691654656 |