Genotoxic damage induced by isopropanol in germinal and somatic cells of Drosophila melanogaster

Isopropanol (isopropyl alcohol, 2-propanol, IPA) is a volatile solvent widely used in domestic or industrial environments and reported as innocuous in various test systems. The aim of this work was to search for in vivo genotoxic effects of IPA in Drosophila melanogaster, studying its ability to ind...

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Autores principales:	Palermo, Ana María, Mudry, Marta Dolores
Publicado:	2011
Materias:	Aneuploidy Drosophila melanogaster Isopropanol (IPA) Meiotic non-disjunction (ND) Sex linked recessive lethals (SLRL) Somatic mutation and recombination (SMART) 2 propanol animal cell animal experiment animal model article cell cycle M phase controlled study female female fertility genotoxicity germ cell male nondisjunction nonhuman priority journal somatic cell somatic mutation spermatogenesis 2-Propanol Animals DNA Damage Female Genes, Lethal Germ Cells Larva Male Mutagenicity Tests Mutagens
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13835718_v726_n2_p215_Palermo http://hdl.handle.net/20.500.12110/paper_13835718_v726_n2_p215_Palermo
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	paper:paper_13835718_v726_n2_p215_Palermo2023-06-08T16:12:29Z Genotoxic damage induced by isopropanol in germinal and somatic cells of Drosophila melanogaster Palermo, Ana María Mudry, Marta Dolores Aneuploidy Drosophila melanogaster Isopropanol (IPA) Meiotic non-disjunction (ND) Sex linked recessive lethals (SLRL) Somatic mutation and recombination (SMART) 2 propanol animal cell animal experiment animal model article cell cycle M phase controlled study Drosophila melanogaster female female fertility genotoxicity germ cell male nondisjunction nonhuman priority journal somatic cell somatic mutation spermatogenesis 2-Propanol Aneuploidy Animals DNA Damage Drosophila melanogaster Female Genes, Lethal Germ Cells Larva Male Mutagenicity Tests Mutagens Drosophila melanogaster Isopropanol (isopropyl alcohol, 2-propanol, IPA) is a volatile solvent widely used in domestic or industrial environments and reported as innocuous in various test systems. The aim of this work was to search for in vivo genotoxic effects of IPA in Drosophila melanogaster, studying its ability to induce nondisjunction (ND) in females, sex linked recessive lethals (SLRL) in males, and somatic mutation and/or recombination (SMART) in larvae. Treatments were acute (60. min) and were administered via inhalation. IPA had low toxicity in adult flies (75% IPA mortality index, MI = 12.7% (females) and 2.6% (males)) and larvae (MI = 14.3%, 75% IPA). Female fertility was severely affected during the first 24 h (brood I, BI) after treatment, but, afterwards, control values were recovered. IPA induced a 50-fold increase of ND (%) in 24 h old females, and a six-fold rise in 4-5 d old BI offspring. Nondisjunction frequencies (%) in the offspring of broods II to V (24 h in each case) were similar to control values. IPA doses of 25% and 50% (v/v), tested in 24 h old females, showed a significant dose-dependent increase of ND(%)in BI only, with control values in subsequent broods. Flies gave normal offspring when kept in regular media for 24 h before mating. The eye spot test (SMART) showed a significant increase at 50% IPA (p<0.05, m = 2), but the response was not dose-dependent. IPA failed to induce SLRL in any of the spermatogenesis stages tested. These findings suggest that the main effect of IPA is to induce chromosomal malsegregation; IPA must be present at the resumption of M-phase I after fertilization, to exert these effects. The alcohol does not affect DNA directly, but perturbations of the nuclear membrane may be responsible for induction of ND. © 2011 Elsevier B.V. Fil:Palermo, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Mudry, M.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2011 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13835718_v726_n2_p215_Palermo http://hdl.handle.net/20.500.12110/paper_13835718_v726_n2_p215_Palermo
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Aneuploidy Drosophila melanogaster Isopropanol (IPA) Meiotic non-disjunction (ND) Sex linked recessive lethals (SLRL) Somatic mutation and recombination (SMART) 2 propanol animal cell animal experiment animal model article cell cycle M phase controlled study Drosophila melanogaster female female fertility genotoxicity germ cell male nondisjunction nonhuman priority journal somatic cell somatic mutation spermatogenesis 2-Propanol Aneuploidy Animals DNA Damage Drosophila melanogaster Female Genes, Lethal Germ Cells Larva Male Mutagenicity Tests Mutagens Drosophila melanogaster
spellingShingle	Aneuploidy Drosophila melanogaster Isopropanol (IPA) Meiotic non-disjunction (ND) Sex linked recessive lethals (SLRL) Somatic mutation and recombination (SMART) 2 propanol animal cell animal experiment animal model article cell cycle M phase controlled study Drosophila melanogaster female female fertility genotoxicity germ cell male nondisjunction nonhuman priority journal somatic cell somatic mutation spermatogenesis 2-Propanol Aneuploidy Animals DNA Damage Drosophila melanogaster Female Genes, Lethal Germ Cells Larva Male Mutagenicity Tests Mutagens Drosophila melanogaster Palermo, Ana María Mudry, Marta Dolores Genotoxic damage induced by isopropanol in germinal and somatic cells of Drosophila melanogaster
topic_facet	Aneuploidy Drosophila melanogaster Isopropanol (IPA) Meiotic non-disjunction (ND) Sex linked recessive lethals (SLRL) Somatic mutation and recombination (SMART) 2 propanol animal cell animal experiment animal model article cell cycle M phase controlled study Drosophila melanogaster female female fertility genotoxicity germ cell male nondisjunction nonhuman priority journal somatic cell somatic mutation spermatogenesis 2-Propanol Aneuploidy Animals DNA Damage Drosophila melanogaster Female Genes, Lethal Germ Cells Larva Male Mutagenicity Tests Mutagens Drosophila melanogaster
description	Isopropanol (isopropyl alcohol, 2-propanol, IPA) is a volatile solvent widely used in domestic or industrial environments and reported as innocuous in various test systems. The aim of this work was to search for in vivo genotoxic effects of IPA in Drosophila melanogaster, studying its ability to induce nondisjunction (ND) in females, sex linked recessive lethals (SLRL) in males, and somatic mutation and/or recombination (SMART) in larvae. Treatments were acute (60. min) and were administered via inhalation. IPA had low toxicity in adult flies (75% IPA mortality index, MI = 12.7% (females) and 2.6% (males)) and larvae (MI = 14.3%, 75% IPA). Female fertility was severely affected during the first 24 h (brood I, BI) after treatment, but, afterwards, control values were recovered. IPA induced a 50-fold increase of ND (%) in 24 h old females, and a six-fold rise in 4-5 d old BI offspring. Nondisjunction frequencies (%) in the offspring of broods II to V (24 h in each case) were similar to control values. IPA doses of 25% and 50% (v/v), tested in 24 h old females, showed a significant dose-dependent increase of ND(%)in BI only, with control values in subsequent broods. Flies gave normal offspring when kept in regular media for 24 h before mating. The eye spot test (SMART) showed a significant increase at 50% IPA (p<0.05, m = 2), but the response was not dose-dependent. IPA failed to induce SLRL in any of the spermatogenesis stages tested. These findings suggest that the main effect of IPA is to induce chromosomal malsegregation; IPA must be present at the resumption of M-phase I after fertilization, to exert these effects. The alcohol does not affect DNA directly, but perturbations of the nuclear membrane may be responsible for induction of ND. © 2011 Elsevier B.V.
author	Palermo, Ana María Mudry, Marta Dolores
author_facet	Palermo, Ana María Mudry, Marta Dolores
author_sort	Palermo, Ana María
title	Genotoxic damage induced by isopropanol in germinal and somatic cells of Drosophila melanogaster
title_short	Genotoxic damage induced by isopropanol in germinal and somatic cells of Drosophila melanogaster
title_full	Genotoxic damage induced by isopropanol in germinal and somatic cells of Drosophila melanogaster
title_fullStr	Genotoxic damage induced by isopropanol in germinal and somatic cells of Drosophila melanogaster
title_full_unstemmed	Genotoxic damage induced by isopropanol in germinal and somatic cells of Drosophila melanogaster
title_sort	genotoxic damage induced by isopropanol in germinal and somatic cells of drosophila melanogaster
publishDate	2011
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13835718_v726_n2_p215_Palermo http://hdl.handle.net/20.500.12110/paper_13835718_v726_n2_p215_Palermo
work_keys_str_mv	AT palermoanamaria genotoxicdamageinducedbyisopropanolingerminalandsomaticcellsofdrosophilamelanogaster AT mudrymartadolores genotoxicdamageinducedbyisopropanolingerminalandsomaticcellsofdrosophilamelanogaster
_version_	1768543527672741888

Genotoxic damage induced by isopropanol in germinal and somatic cells of Drosophila melanogaster

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