Genetic characterization of apospory in tetraploid Paspalum notatum based on the identification of linked molecular markers

Tetraploid Paspalum notatum (bahiagrass) is a valuable forage grass with aposporous apomictic reproduction. In a previous study, we showed that apospory in bahiagrass is under the control of a single dominant gene with a distorted segregation ratio. The objective of this work was to identify molecul...

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Autores principales:	Martinez, Eric Javier, Hopp, Horacio Esteban
Publicado:	2003
Materias:	Apomixis Apospory Bulked segregant analysis Comparative mapping Molecular markers Paspalum notatum Embryophyta Paspalum Paspalum simplex
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13803743_v12_n4_p319_Martinez http://hdl.handle.net/20.500.12110/paper_13803743_v12_n4_p319_Martinez
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_13803743_v12_n4_p319_Martinez
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spelling	paper:paper_13803743_v12_n4_p319_Martinez2023-06-08T16:12:16Z Genetic characterization of apospory in tetraploid Paspalum notatum based on the identification of linked molecular markers Martinez, Eric Javier Hopp, Horacio Esteban Apomixis Apospory Bulked segregant analysis Comparative mapping Molecular markers Paspalum notatum Embryophyta Paspalum Paspalum notatum Paspalum simplex Tetraploid Paspalum notatum (bahiagrass) is a valuable forage grass with aposporous apomictic reproduction. In a previous study, we showed that apospory in bahiagrass is under the control of a single dominant gene with a distorted segregation ratio. The objective of this work was to identify molecular markers linked to apospory in tetraploid P. notatum and establish a preliminary syntenic relationship with the genomic region associated with apospory in P. simplex. A F1 population of 290 individuals, segregating for apospory, was generated after crossing a completely sexual plant (Q4188) with a natural aposporous apomictic plant (Q4117). The whole progeny was classified as sexual or aposporous by embryo sacs analysis. A bulked segregant analysis was carried out to identify molecular markers co-segregating with apospory. Four hundred RAPD primers, 30 AFLP primers combinations and 85 RFLP clones were screened using DNA from both parental genotypes and aposporous and sexual bulks. Linkage analysis was performed with cytological and genetic information from the complete progeny. Cytoembryological analysis showed 219 sexual and 71 aposporous F1 individuals. Seven different molecular markers (2 RAPD, 4 AFLP and 1 RFLP) were found to be completely linked to apospory. The RFLP probe C1069, mapping to the telomeric region of the long arm of rice chromosome 12, was one of the molecular markers completely linked to apospory in P. notatum. This marker had been previously associated with apospory in P. simplex. A preliminary map of the chromosome region carrying the apospory locus was constructed. Fil:Martínez, E.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Hopp, H.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2003 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13803743_v12_n4_p319_Martinez http://hdl.handle.net/20.500.12110/paper_13803743_v12_n4_p319_Martinez
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Apomixis Apospory Bulked segregant analysis Comparative mapping Molecular markers Paspalum notatum Embryophyta Paspalum Paspalum notatum Paspalum simplex
spellingShingle	Apomixis Apospory Bulked segregant analysis Comparative mapping Molecular markers Paspalum notatum Embryophyta Paspalum Paspalum notatum Paspalum simplex Martinez, Eric Javier Hopp, Horacio Esteban Genetic characterization of apospory in tetraploid Paspalum notatum based on the identification of linked molecular markers
topic_facet	Apomixis Apospory Bulked segregant analysis Comparative mapping Molecular markers Paspalum notatum Embryophyta Paspalum Paspalum notatum Paspalum simplex
description	Tetraploid Paspalum notatum (bahiagrass) is a valuable forage grass with aposporous apomictic reproduction. In a previous study, we showed that apospory in bahiagrass is under the control of a single dominant gene with a distorted segregation ratio. The objective of this work was to identify molecular markers linked to apospory in tetraploid P. notatum and establish a preliminary syntenic relationship with the genomic region associated with apospory in P. simplex. A F1 population of 290 individuals, segregating for apospory, was generated after crossing a completely sexual plant (Q4188) with a natural aposporous apomictic plant (Q4117). The whole progeny was classified as sexual or aposporous by embryo sacs analysis. A bulked segregant analysis was carried out to identify molecular markers co-segregating with apospory. Four hundred RAPD primers, 30 AFLP primers combinations and 85 RFLP clones were screened using DNA from both parental genotypes and aposporous and sexual bulks. Linkage analysis was performed with cytological and genetic information from the complete progeny. Cytoembryological analysis showed 219 sexual and 71 aposporous F1 individuals. Seven different molecular markers (2 RAPD, 4 AFLP and 1 RFLP) were found to be completely linked to apospory. The RFLP probe C1069, mapping to the telomeric region of the long arm of rice chromosome 12, was one of the molecular markers completely linked to apospory in P. notatum. This marker had been previously associated with apospory in P. simplex. A preliminary map of the chromosome region carrying the apospory locus was constructed.
author	Martinez, Eric Javier Hopp, Horacio Esteban
author_facet	Martinez, Eric Javier Hopp, Horacio Esteban
author_sort	Martinez, Eric Javier
title	Genetic characterization of apospory in tetraploid Paspalum notatum based on the identification of linked molecular markers
title_short	Genetic characterization of apospory in tetraploid Paspalum notatum based on the identification of linked molecular markers
title_full	Genetic characterization of apospory in tetraploid Paspalum notatum based on the identification of linked molecular markers
title_fullStr	Genetic characterization of apospory in tetraploid Paspalum notatum based on the identification of linked molecular markers
title_full_unstemmed	Genetic characterization of apospory in tetraploid Paspalum notatum based on the identification of linked molecular markers
title_sort	genetic characterization of apospory in tetraploid paspalum notatum based on the identification of linked molecular markers
publishDate	2003
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13803743_v12_n4_p319_Martinez http://hdl.handle.net/20.500.12110/paper_13803743_v12_n4_p319_Martinez
work_keys_str_mv	AT martinezericjavier geneticcharacterizationofaposporyintetraploidpaspalumnotatumbasedontheidentificationoflinkedmolecularmarkers AT hopphoracioesteban geneticcharacterizationofaposporyintetraploidpaspalumnotatumbasedontheidentificationoflinkedmolecularmarkers
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Genetic characterization of apospory in tetraploid Paspalum notatum based on the identification of linked molecular markers

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