Optimization of lignocellulolytic enzyme production by the white-rot fungus Trametes trogii in solid-state fermentation using response surface methodology
The white-rot basidiomicete Trametes trogii (MYA 28-11) is an outstanding producer of laccase. A Doehlert experimental design was applied to optimize its lignocellulolytic enzyme production in solid-state fermentation. The impact on enzyme production of three quantitative variables, namely pH, coppe...
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paper:paper_1369703X_v39_n1_p207_Levin2023-06-08T16:12:14Z Optimization of lignocellulolytic enzyme production by the white-rot fungus Trametes trogii in solid-state fermentation using response surface methodology Levin, Laura Noemi Papinutti, Víctor Leandro Lignocellulolytic enzymes Response surface methodology Solid-state fermentation White-rot fungi Fermentation Fungi pH effects Solid state reactions Lignocellulolytic enzymes Response surface methodology Solid state fermentation White-rot fungi Enzyme activity cellulase copper laccase lignocellulose manganese peroxidase nitrogen peptone xylan endo 1,3 beta xylosidase acidity article bleaching correlation analysis culture medium enzyme activity enzyme synthesis experimental design malt nonhuman paper industry pH priority journal pulp processing response surface method solid state fermentation Trametes Trametes trogii white rot fungus wood Funalia trogii Fungi Mya The white-rot basidiomicete Trametes trogii (MYA 28-11) is an outstanding producer of laccase. A Doehlert experimental design was applied to optimize its lignocellulolytic enzyme production in solid-state fermentation. The impact on enzyme production of three quantitative variables, namely pH, copper and nitrogen concentrations, was investigated by using a wood-based solid medium supplemented with malt extract. Optimization was aimed at simultaneously minimizing cellulase activity and maximizing ligninolytic enzyme production. Such conditions were: pH 4.5, peptone 12.5 g l-1, and CuSO4 11 mM. Highest activities achieved were: laccase 901 and Mn-peroxidase 20 U g-1. There was not a direct correlation between the levels of enzymatic activities and the extent of losses of wood weight or components. The factorial approach also allowed us to quantify the enzyme activity in any part of the experimental domain, consequently, it was also possible to determine a culture medium to obtain crude extracellular extracts with high laccase (510 U g-1) and endoxylanase (780 U g-1) yields. These enzymes have gained renewed interest mainly due to their applications in paper industries for pulp treatment, improving the effectiveness of conventional bleaching. Considering the results obtained, T. trogii could be an attractive source of both enzymes. © 2007 Elsevier B.V. All rights reserved. Fil:Levin, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Papinutti, V.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2008 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1369703X_v39_n1_p207_Levin http://hdl.handle.net/20.500.12110/paper_1369703X_v39_n1_p207_Levin |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Lignocellulolytic enzymes Response surface methodology Solid-state fermentation White-rot fungi Fermentation Fungi pH effects Solid state reactions Lignocellulolytic enzymes Response surface methodology Solid state fermentation White-rot fungi Enzyme activity cellulase copper laccase lignocellulose manganese peroxidase nitrogen peptone xylan endo 1,3 beta xylosidase acidity article bleaching correlation analysis culture medium enzyme activity enzyme synthesis experimental design malt nonhuman paper industry pH priority journal pulp processing response surface method solid state fermentation Trametes Trametes trogii white rot fungus wood Funalia trogii Fungi Mya |
| spellingShingle |
Lignocellulolytic enzymes Response surface methodology Solid-state fermentation White-rot fungi Fermentation Fungi pH effects Solid state reactions Lignocellulolytic enzymes Response surface methodology Solid state fermentation White-rot fungi Enzyme activity cellulase copper laccase lignocellulose manganese peroxidase nitrogen peptone xylan endo 1,3 beta xylosidase acidity article bleaching correlation analysis culture medium enzyme activity enzyme synthesis experimental design malt nonhuman paper industry pH priority journal pulp processing response surface method solid state fermentation Trametes Trametes trogii white rot fungus wood Funalia trogii Fungi Mya Levin, Laura Noemi Papinutti, Víctor Leandro Optimization of lignocellulolytic enzyme production by the white-rot fungus Trametes trogii in solid-state fermentation using response surface methodology |
| topic_facet |
Lignocellulolytic enzymes Response surface methodology Solid-state fermentation White-rot fungi Fermentation Fungi pH effects Solid state reactions Lignocellulolytic enzymes Response surface methodology Solid state fermentation White-rot fungi Enzyme activity cellulase copper laccase lignocellulose manganese peroxidase nitrogen peptone xylan endo 1,3 beta xylosidase acidity article bleaching correlation analysis culture medium enzyme activity enzyme synthesis experimental design malt nonhuman paper industry pH priority journal pulp processing response surface method solid state fermentation Trametes Trametes trogii white rot fungus wood Funalia trogii Fungi Mya |
| description |
The white-rot basidiomicete Trametes trogii (MYA 28-11) is an outstanding producer of laccase. A Doehlert experimental design was applied to optimize its lignocellulolytic enzyme production in solid-state fermentation. The impact on enzyme production of three quantitative variables, namely pH, copper and nitrogen concentrations, was investigated by using a wood-based solid medium supplemented with malt extract. Optimization was aimed at simultaneously minimizing cellulase activity and maximizing ligninolytic enzyme production. Such conditions were: pH 4.5, peptone 12.5 g l-1, and CuSO4 11 mM. Highest activities achieved were: laccase 901 and Mn-peroxidase 20 U g-1. There was not a direct correlation between the levels of enzymatic activities and the extent of losses of wood weight or components. The factorial approach also allowed us to quantify the enzyme activity in any part of the experimental domain, consequently, it was also possible to determine a culture medium to obtain crude extracellular extracts with high laccase (510 U g-1) and endoxylanase (780 U g-1) yields. These enzymes have gained renewed interest mainly due to their applications in paper industries for pulp treatment, improving the effectiveness of conventional bleaching. Considering the results obtained, T. trogii could be an attractive source of both enzymes. © 2007 Elsevier B.V. All rights reserved. |
| author |
Levin, Laura Noemi Papinutti, Víctor Leandro |
| author_facet |
Levin, Laura Noemi Papinutti, Víctor Leandro |
| author_sort |
Levin, Laura Noemi |
| title |
Optimization of lignocellulolytic enzyme production by the white-rot fungus Trametes trogii in solid-state fermentation using response surface methodology |
| title_short |
Optimization of lignocellulolytic enzyme production by the white-rot fungus Trametes trogii in solid-state fermentation using response surface methodology |
| title_full |
Optimization of lignocellulolytic enzyme production by the white-rot fungus Trametes trogii in solid-state fermentation using response surface methodology |
| title_fullStr |
Optimization of lignocellulolytic enzyme production by the white-rot fungus Trametes trogii in solid-state fermentation using response surface methodology |
| title_full_unstemmed |
Optimization of lignocellulolytic enzyme production by the white-rot fungus Trametes trogii in solid-state fermentation using response surface methodology |
| title_sort |
optimization of lignocellulolytic enzyme production by the white-rot fungus trametes trogii in solid-state fermentation using response surface methodology |
| publishDate |
2008 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1369703X_v39_n1_p207_Levin http://hdl.handle.net/20.500.12110/paper_1369703X_v39_n1_p207_Levin |
| work_keys_str_mv |
AT levinlauranoemi optimizationoflignocellulolyticenzymeproductionbythewhiterotfungustrametestrogiiinsolidstatefermentationusingresponsesurfacemethodology AT papinuttivictorleandro optimizationoflignocellulolyticenzymeproductionbythewhiterotfungustrametestrogiiinsolidstatefermentationusingresponsesurfacemethodology |
| _version_ |
1768546688638648320 |