Optimization of manganese peroxidase and laccase production in the South American fungus Fomes sclerodermeus (Lév.) Cke
Fomes sclerodermeus produces manganese peroxidase (MnP) and laccase as part of its ligninolytic system. A Doehlert experimental design was applied in order to find the optimum conditions for MnP and laccase production. The factors studied were Cu2+, Mn2+ and asparagine. The present model and data an...
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2003
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13675435_v30_n9_p536_Papinutti http://hdl.handle.net/20.500.12110/paper_13675435_v30_n9_p536_Papinutti |
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paper:paper_13675435_v30_n9_p536_Papinutti2023-06-08T16:12:12Z Optimization of manganese peroxidase and laccase production in the South American fungus Fomes sclerodermeus (Lév.) Cke Fomes sclerodermeus Laccase Manganese peroxidase Statistical experimental design asparagine copper ion copper sulfate laccase manganese manganese peroxidase manganese sulfate article controlled study enzyme synthesis experimental design Fomes scleroderneus fungus fungus culture fungus growth model nonhuman nutrient process optimization South America Asparagine Biomass Biotechnology Copper Culture Media Fungi Laccase Lignin Manganese Models, Biological Peroxidases South America Fomes Fungi Fomes sclerodermeus produces manganese peroxidase (MnP) and laccase as part of its ligninolytic system. A Doehlert experimental design was applied in order to find the optimum conditions for MnP and laccase production. The factors studied were Cu2+, Mn2+ and asparagine. The present model and data analysis allowed us not only to define optimal media for production of both laccase and MnP, but also to show the combined effects between the factors. MnP was strongly influenced by Mn2+, which acts as an inducer. Under these conditions Cu2+ negatively affected MnP activity. At 13 days of growth 0.75 U ml-1 were produced in the optimized culture medium supplemented with 1 mM MnSO4 and 4 g l -1 asparagine. The laccase titer under optimized conditions reached maximum values at 16 days of growth: 13.5 U ml-1 in the presence of 0.2 mM CuSO4, 0.4 mM MnSO4 and 6 g l-1 asparagine. Mn2+ promoted production of both enzymes. There were important interactions among the nutrients evaluated, the most significant being those between Cu2+ and asparagine. 2003 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13675435_v30_n9_p536_Papinutti http://hdl.handle.net/20.500.12110/paper_13675435_v30_n9_p536_Papinutti |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Fomes sclerodermeus Laccase Manganese peroxidase Statistical experimental design asparagine copper ion copper sulfate laccase manganese manganese peroxidase manganese sulfate article controlled study enzyme synthesis experimental design Fomes scleroderneus fungus fungus culture fungus growth model nonhuman nutrient process optimization South America Asparagine Biomass Biotechnology Copper Culture Media Fungi Laccase Lignin Manganese Models, Biological Peroxidases South America Fomes Fungi |
| spellingShingle |
Fomes sclerodermeus Laccase Manganese peroxidase Statistical experimental design asparagine copper ion copper sulfate laccase manganese manganese peroxidase manganese sulfate article controlled study enzyme synthesis experimental design Fomes scleroderneus fungus fungus culture fungus growth model nonhuman nutrient process optimization South America Asparagine Biomass Biotechnology Copper Culture Media Fungi Laccase Lignin Manganese Models, Biological Peroxidases South America Fomes Fungi Optimization of manganese peroxidase and laccase production in the South American fungus Fomes sclerodermeus (Lév.) Cke |
| topic_facet |
Fomes sclerodermeus Laccase Manganese peroxidase Statistical experimental design asparagine copper ion copper sulfate laccase manganese manganese peroxidase manganese sulfate article controlled study enzyme synthesis experimental design Fomes scleroderneus fungus fungus culture fungus growth model nonhuman nutrient process optimization South America Asparagine Biomass Biotechnology Copper Culture Media Fungi Laccase Lignin Manganese Models, Biological Peroxidases South America Fomes Fungi |
| description |
Fomes sclerodermeus produces manganese peroxidase (MnP) and laccase as part of its ligninolytic system. A Doehlert experimental design was applied in order to find the optimum conditions for MnP and laccase production. The factors studied were Cu2+, Mn2+ and asparagine. The present model and data analysis allowed us not only to define optimal media for production of both laccase and MnP, but also to show the combined effects between the factors. MnP was strongly influenced by Mn2+, which acts as an inducer. Under these conditions Cu2+ negatively affected MnP activity. At 13 days of growth 0.75 U ml-1 were produced in the optimized culture medium supplemented with 1 mM MnSO4 and 4 g l -1 asparagine. The laccase titer under optimized conditions reached maximum values at 16 days of growth: 13.5 U ml-1 in the presence of 0.2 mM CuSO4, 0.4 mM MnSO4 and 6 g l-1 asparagine. Mn2+ promoted production of both enzymes. There were important interactions among the nutrients evaluated, the most significant being those between Cu2+ and asparagine. |
| title |
Optimization of manganese peroxidase and laccase production in the South American fungus Fomes sclerodermeus (Lév.) Cke |
| title_short |
Optimization of manganese peroxidase and laccase production in the South American fungus Fomes sclerodermeus (Lév.) Cke |
| title_full |
Optimization of manganese peroxidase and laccase production in the South American fungus Fomes sclerodermeus (Lév.) Cke |
| title_fullStr |
Optimization of manganese peroxidase and laccase production in the South American fungus Fomes sclerodermeus (Lév.) Cke |
| title_full_unstemmed |
Optimization of manganese peroxidase and laccase production in the South American fungus Fomes sclerodermeus (Lév.) Cke |
| title_sort |
optimization of manganese peroxidase and laccase production in the south american fungus fomes sclerodermeus (lév.) cke |
| publishDate |
2003 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13675435_v30_n9_p536_Papinutti http://hdl.handle.net/20.500.12110/paper_13675435_v30_n9_p536_Papinutti |
| _version_ |
1768542519782539264 |