Production of laccase and manganese peroxidase by Fomes sclerodermeus grown on wheat bran

The aim of this work was to study the growth and production of ligninolytic enzymes by Fomes sclerodermeus using a natural medium based on wheat bran as the principal substrate in a solid-state fermentation. Growth was monitored by measuring the chitin content in the substrate. The maximum rate of g...

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Autores principales:	Papinutti, Víctor Leandro, Diorio, Luis Alberto, Forchíassin, Flavia
Publicado:	2003
Materias:	Fermentation Fomes sclerodermeus Ligninases Lignocellulosic cellulose fungal enzyme laccase lignin lignin peroxidase lignocellulose manganese peroxidase article Basidiomycetes enzyme activity enzyme assay enzyme substrate fermentation fungus culture fungus growth hydrolysis nonhuman nutrient wheat bran Basidiomycota Fomes Fungi Triticum aestivum
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13675435_v30_n3_p157_Papinutti http://hdl.handle.net/20.500.12110/paper_13675435_v30_n3_p157_Papinutti
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_13675435_v30_n3_p157_Papinutti
record_format	dspace
spelling	paper:paper_13675435_v30_n3_p157_Papinutti2023-06-08T16:12:12Z Production of laccase and manganese peroxidase by Fomes sclerodermeus grown on wheat bran Papinutti, Víctor Leandro Diorio, Luis Alberto Forchíassin, Flavia Fermentation Fomes sclerodermeus Ligninases Lignocellulosic cellulose fungal enzyme laccase lignin lignin peroxidase lignocellulose manganese peroxidase article Basidiomycetes enzyme activity enzyme assay enzyme substrate fermentation Fomes sclerodermeus fungus culture fungus growth hydrolysis nonhuman nutrient wheat bran Basidiomycota Fomes Fungi Triticum aestivum The aim of this work was to study the growth and production of ligninolytic enzymes by Fomes sclerodermeus using a natural medium based on wheat bran as the principal substrate in a solid-state fermentation. Growth was monitored by measuring the chitin content in the substrate. The maximum rate of growth was observed between days 7 and 18. A 38% total dry-weight loss of the substrate was measured after 28 days of cultivation. Differential hydrolysis of the substrate revealed that cellulose was more extensively degraded than lignin. In the 28-day incubation period, the losses of cellulose and lignin were 38 and 15%, respectively. No lignin peroxidase activity was found in any of the media tested. The maximum manganese-dependent peroxidase activity recorded was 6.3 U g-1 at 14 days, while the maximum laccase activity was 270 U g-1 at 28 days post-inoculation. Addition of commonly used inducers such as copper or manganese did not produce a further increase in the enzyme activities, nor did addition of glucose, asparagine, or malt extract. Fil:Papinutti, V.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Diorio, L.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Forchiassin, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2003 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13675435_v30_n3_p157_Papinutti http://hdl.handle.net/20.500.12110/paper_13675435_v30_n3_p157_Papinutti
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Fermentation Fomes sclerodermeus Ligninases Lignocellulosic cellulose fungal enzyme laccase lignin lignin peroxidase lignocellulose manganese peroxidase article Basidiomycetes enzyme activity enzyme assay enzyme substrate fermentation Fomes sclerodermeus fungus culture fungus growth hydrolysis nonhuman nutrient wheat bran Basidiomycota Fomes Fungi Triticum aestivum
spellingShingle	Fermentation Fomes sclerodermeus Ligninases Lignocellulosic cellulose fungal enzyme laccase lignin lignin peroxidase lignocellulose manganese peroxidase article Basidiomycetes enzyme activity enzyme assay enzyme substrate fermentation Fomes sclerodermeus fungus culture fungus growth hydrolysis nonhuman nutrient wheat bran Basidiomycota Fomes Fungi Triticum aestivum Papinutti, Víctor Leandro Diorio, Luis Alberto Forchíassin, Flavia Production of laccase and manganese peroxidase by Fomes sclerodermeus grown on wheat bran
topic_facet	Fermentation Fomes sclerodermeus Ligninases Lignocellulosic cellulose fungal enzyme laccase lignin lignin peroxidase lignocellulose manganese peroxidase article Basidiomycetes enzyme activity enzyme assay enzyme substrate fermentation Fomes sclerodermeus fungus culture fungus growth hydrolysis nonhuman nutrient wheat bran Basidiomycota Fomes Fungi Triticum aestivum
description	The aim of this work was to study the growth and production of ligninolytic enzymes by Fomes sclerodermeus using a natural medium based on wheat bran as the principal substrate in a solid-state fermentation. Growth was monitored by measuring the chitin content in the substrate. The maximum rate of growth was observed between days 7 and 18. A 38% total dry-weight loss of the substrate was measured after 28 days of cultivation. Differential hydrolysis of the substrate revealed that cellulose was more extensively degraded than lignin. In the 28-day incubation period, the losses of cellulose and lignin were 38 and 15%, respectively. No lignin peroxidase activity was found in any of the media tested. The maximum manganese-dependent peroxidase activity recorded was 6.3 U g-1 at 14 days, while the maximum laccase activity was 270 U g-1 at 28 days post-inoculation. Addition of commonly used inducers such as copper or manganese did not produce a further increase in the enzyme activities, nor did addition of glucose, asparagine, or malt extract.
author	Papinutti, Víctor Leandro Diorio, Luis Alberto Forchíassin, Flavia
author_facet	Papinutti, Víctor Leandro Diorio, Luis Alberto Forchíassin, Flavia
author_sort	Papinutti, Víctor Leandro
title	Production of laccase and manganese peroxidase by Fomes sclerodermeus grown on wheat bran
title_short	Production of laccase and manganese peroxidase by Fomes sclerodermeus grown on wheat bran
title_full	Production of laccase and manganese peroxidase by Fomes sclerodermeus grown on wheat bran
title_fullStr	Production of laccase and manganese peroxidase by Fomes sclerodermeus grown on wheat bran
title_full_unstemmed	Production of laccase and manganese peroxidase by Fomes sclerodermeus grown on wheat bran
title_sort	production of laccase and manganese peroxidase by fomes sclerodermeus grown on wheat bran
publishDate	2003
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13675435_v30_n3_p157_Papinutti http://hdl.handle.net/20.500.12110/paper_13675435_v30_n3_p157_Papinutti
work_keys_str_mv	AT papinuttivictorleandro productionoflaccaseandmanganeseperoxidasebyfomessclerodermeusgrownonwheatbran AT diorioluisalberto productionoflaccaseandmanganeseperoxidasebyfomessclerodermeusgrownonwheatbran AT forchiassinflavia productionoflaccaseandmanganeseperoxidasebyfomessclerodermeusgrownonwheatbran
_version_	1768546130989154304

Production of laccase and manganese peroxidase by Fomes sclerodermeus grown on wheat bran

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