Improved robustness of an ethanologenic yeast strain through adaptive evolution in acetic acid is associated with its enzymatic antioxidant ability
Aims: To investigate multiple tolerance of Saccharomyces cerevisiae obtained through a laboratory strategy of adaptive evolution in acetic acid, its relation with enzymatic ROS detoxification and bioethanol 2G production. Methods and Results: After adaptive evolution in acetic acid, a clone (Y8A) wa...
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2018
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13645072_v125_n3_p766_Gurdo http://hdl.handle.net/20.500.12110/paper_13645072_v125_n3_p766_Gurdo |
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paper:paper_13645072_v125_n3_p766_Gurdo2023-06-08T16:11:46Z Improved robustness of an ethanologenic yeast strain through adaptive evolution in acetic acid is associated with its enzymatic antioxidant ability acetic acid adaptive evolution antioxidative enzymes bioethanol 2G production multiple tolerance robustness Saccharomyces cerevisiae yeast acetic acid alcohol bioethanol carboxylic acid catalase glutathione transferase phenol derivative protein reactive oxygen metabolite sodium chloride acetic acid antioxidant Saccharomyces cerevisiae protein acetic acid adaptive radiation antioxidant biofuel concentration (composition) detoxification enzyme enzyme activity evolutionary biology homeostasis oxidative stress tolerance yeast adaptation antioxidant activity Article biofuel production clone cross tolerance enzymatic assay enzyme activity freeze thawing fungal strain fungus culture nonhuman osmotic stress oxidative stress protein content Saccharomyces cerevisiae stress drug effect enzymology metabolism physiology Saccharomyces cerevisiae Acetic Acid Antioxidants Ethanol Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Aims: To investigate multiple tolerance of Saccharomyces cerevisiae obtained through a laboratory strategy of adaptive evolution in acetic acid, its relation with enzymatic ROS detoxification and bioethanol 2G production. Methods and Results: After adaptive evolution in acetic acid, a clone (Y8A) was selected for its tolerance to high acetic acid concentrations (13 g l−1) in batch cultures. Y8A was resistant to multiple stresses: osmotic, thermic, oxidative, saline, ethanol, organic acid, phenolic compounds and slow freeze-thawing cycles. Also, Y8A was able to maintain redox homeostasis under oxidative stress, whereas the isogenic parental strain (Y8) could not, indicating higher basal activity levels of antioxidative enzyme Catalase (CAT) and Gluthatione S-transferase (GST) in Y8A. Y8A reached higher bioethanol levels in a fermentation medium containing up to 8 g l−1 of acetic acid when compared to parental strain Y8. Conclusions: A multiple-stress-tolerant clone was obtained using adaptive evolution in acetic acid. Stress cross-tolerance could be explained by its enzymatic antioxidative capacity, namely CAT and GST. Significance and Impact of the Study: We demonstrate that adaptive evolution used in S. cerevisiae was a useful strategy to obtain a yeast clone tolerant to multiple stresses. At the same time, our findings support the idea that tolerance to oxidative stress is the common basis for stress cotolerance, which is related to an increase in the specific enzymes CAT and GST but not in Superoxide dismutase, emphasizing the fact that detoxification of H2O2 and not O2˙ is a key condition for multiple stress tolerance in S. cerevisiae. © 2018 The Society for Applied Microbiology 2018 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13645072_v125_n3_p766_Gurdo http://hdl.handle.net/20.500.12110/paper_13645072_v125_n3_p766_Gurdo |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
acetic acid adaptive evolution antioxidative enzymes bioethanol 2G production multiple tolerance robustness Saccharomyces cerevisiae yeast acetic acid alcohol bioethanol carboxylic acid catalase glutathione transferase phenol derivative protein reactive oxygen metabolite sodium chloride acetic acid antioxidant Saccharomyces cerevisiae protein acetic acid adaptive radiation antioxidant biofuel concentration (composition) detoxification enzyme enzyme activity evolutionary biology homeostasis oxidative stress tolerance yeast adaptation antioxidant activity Article biofuel production clone cross tolerance enzymatic assay enzyme activity freeze thawing fungal strain fungus culture nonhuman osmotic stress oxidative stress protein content Saccharomyces cerevisiae stress drug effect enzymology metabolism physiology Saccharomyces cerevisiae Acetic Acid Antioxidants Ethanol Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins |
| spellingShingle |
acetic acid adaptive evolution antioxidative enzymes bioethanol 2G production multiple tolerance robustness Saccharomyces cerevisiae yeast acetic acid alcohol bioethanol carboxylic acid catalase glutathione transferase phenol derivative protein reactive oxygen metabolite sodium chloride acetic acid antioxidant Saccharomyces cerevisiae protein acetic acid adaptive radiation antioxidant biofuel concentration (composition) detoxification enzyme enzyme activity evolutionary biology homeostasis oxidative stress tolerance yeast adaptation antioxidant activity Article biofuel production clone cross tolerance enzymatic assay enzyme activity freeze thawing fungal strain fungus culture nonhuman osmotic stress oxidative stress protein content Saccharomyces cerevisiae stress drug effect enzymology metabolism physiology Saccharomyces cerevisiae Acetic Acid Antioxidants Ethanol Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Improved robustness of an ethanologenic yeast strain through adaptive evolution in acetic acid is associated with its enzymatic antioxidant ability |
| topic_facet |
acetic acid adaptive evolution antioxidative enzymes bioethanol 2G production multiple tolerance robustness Saccharomyces cerevisiae yeast acetic acid alcohol bioethanol carboxylic acid catalase glutathione transferase phenol derivative protein reactive oxygen metabolite sodium chloride acetic acid antioxidant Saccharomyces cerevisiae protein acetic acid adaptive radiation antioxidant biofuel concentration (composition) detoxification enzyme enzyme activity evolutionary biology homeostasis oxidative stress tolerance yeast adaptation antioxidant activity Article biofuel production clone cross tolerance enzymatic assay enzyme activity freeze thawing fungal strain fungus culture nonhuman osmotic stress oxidative stress protein content Saccharomyces cerevisiae stress drug effect enzymology metabolism physiology Saccharomyces cerevisiae Acetic Acid Antioxidants Ethanol Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins |
| description |
Aims: To investigate multiple tolerance of Saccharomyces cerevisiae obtained through a laboratory strategy of adaptive evolution in acetic acid, its relation with enzymatic ROS detoxification and bioethanol 2G production. Methods and Results: After adaptive evolution in acetic acid, a clone (Y8A) was selected for its tolerance to high acetic acid concentrations (13 g l−1) in batch cultures. Y8A was resistant to multiple stresses: osmotic, thermic, oxidative, saline, ethanol, organic acid, phenolic compounds and slow freeze-thawing cycles. Also, Y8A was able to maintain redox homeostasis under oxidative stress, whereas the isogenic parental strain (Y8) could not, indicating higher basal activity levels of antioxidative enzyme Catalase (CAT) and Gluthatione S-transferase (GST) in Y8A. Y8A reached higher bioethanol levels in a fermentation medium containing up to 8 g l−1 of acetic acid when compared to parental strain Y8. Conclusions: A multiple-stress-tolerant clone was obtained using adaptive evolution in acetic acid. Stress cross-tolerance could be explained by its enzymatic antioxidative capacity, namely CAT and GST. Significance and Impact of the Study: We demonstrate that adaptive evolution used in S. cerevisiae was a useful strategy to obtain a yeast clone tolerant to multiple stresses. At the same time, our findings support the idea that tolerance to oxidative stress is the common basis for stress cotolerance, which is related to an increase in the specific enzymes CAT and GST but not in Superoxide dismutase, emphasizing the fact that detoxification of H2O2 and not O2˙ is a key condition for multiple stress tolerance in S. cerevisiae. © 2018 The Society for Applied Microbiology |
| title |
Improved robustness of an ethanologenic yeast strain through adaptive evolution in acetic acid is associated with its enzymatic antioxidant ability |
| title_short |
Improved robustness of an ethanologenic yeast strain through adaptive evolution in acetic acid is associated with its enzymatic antioxidant ability |
| title_full |
Improved robustness of an ethanologenic yeast strain through adaptive evolution in acetic acid is associated with its enzymatic antioxidant ability |
| title_fullStr |
Improved robustness of an ethanologenic yeast strain through adaptive evolution in acetic acid is associated with its enzymatic antioxidant ability |
| title_full_unstemmed |
Improved robustness of an ethanologenic yeast strain through adaptive evolution in acetic acid is associated with its enzymatic antioxidant ability |
| title_sort |
improved robustness of an ethanologenic yeast strain through adaptive evolution in acetic acid is associated with its enzymatic antioxidant ability |
| publishDate |
2018 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13645072_v125_n3_p766_Gurdo http://hdl.handle.net/20.500.12110/paper_13645072_v125_n3_p766_Gurdo |
| _version_ |
1768542234112688128 |