Antiherpes simplex virus type 2 activity of the antimicrobial peptide subtilosin

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Aims: In this study, we evaluated the antiviral activity of subtilosin, a cyclical peptide isolated from Bacillus amyloliquefaciens, against herpes simplex virus type 2 (HSV-2) in cell cultures and we investigated subtilosin mode of action. Methods and Results: We determined, using a virus yield inh...

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Autores principales:	Torres, Nicolás I., Wachsman, Mónica Beatriz, Castilla, Viviana
Publicado:	2014
Materias:	Antimicrobial peptide Antiviral activity Bacteriocin Herpes simplex virus Microbicide Subtilosin antivirus agent bacteriocin glycoprotein glycoprotein gd polypeptide antibiotic agent subtilosin unclassified drug cyclopeptide sboA protein, Bacillus subtilis animal cell antiviral activity Article Bacillus amyloliquefaciens cell culture cell viability cellular distribution controlled study cytotoxicity dose response drug mechanism Herpes simplex virus 2 immunofluorescence test intracellular space intracellular transport nonhuman protein expression protein isolation Vero cell line virus particle virus release virus replication Western blotting animal Chlorocebus aethiops drug effects human Simplexvirus Animals Antiviral Agents Bacteriocins Cercopithecus aethiops Herpesvirus 2, Human Humans Peptides, Cyclic Vero Cells
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13645072_v117_n5_p1253_Quintana http://hdl.handle.net/20.500.12110/paper_13645072_v117_n5_p1253_Quintana
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	paper:paper_13645072_v117_n5_p1253_Quintana2023-06-08T16:11:45Z Antiherpes simplex virus type 2 activity of the antimicrobial peptide subtilosin Torres, Nicolás I. Wachsman, Mónica Beatriz Castilla, Viviana Antimicrobial peptide Antiviral activity Bacteriocin Herpes simplex virus Microbicide Subtilosin antivirus agent bacteriocin glycoprotein glycoprotein gd polypeptide antibiotic agent subtilosin unclassified drug antivirus agent bacteriocin cyclopeptide sboA protein, Bacillus subtilis animal cell antiviral activity Article Bacillus amyloliquefaciens cell culture cell viability cellular distribution controlled study cytotoxicity dose response drug mechanism Herpes simplex virus 2 immunofluorescence test intracellular space intracellular transport nonhuman protein expression protein isolation Vero cell line virus particle virus release virus replication Western blotting animal Chlorocebus aethiops drug effects Herpes simplex virus 2 human Simplexvirus Animals Antiviral Agents Bacteriocins Cercopithecus aethiops Herpesvirus 2, Human Humans Peptides, Cyclic Vero Cells Aims: In this study, we evaluated the antiviral activity of subtilosin, a cyclical peptide isolated from Bacillus amyloliquefaciens, against herpes simplex virus type 2 (HSV-2) in cell cultures and we investigated subtilosin mode of action. Methods and Results: We determined, using a virus yield inhibition assay, that noncytotoxic concentrations of subtilosin inhibit HSV-2 replication in Vero cell cultures. Subtilosin strongly inhibited extracellular and total virus production even when it was added at 8 h postinfection indicating that not only virus release but also viral particle formation is impeded by the antiviral peptide. Although viral glycoprotein gD level of expression is not affected by the bacteriocin, an altered pattern of gD intracellular localization was detected by immunofluorescence assay in subtilosin-treated culture. On the other hand, at high concentrations, subtilosin displays virucidal action. Conclusions: Subtilosin displays antiviral and virucidal actions against HSV-2. The target of subtilosin inhibitory effect would be late stages of the viral replicative cycle such as viral glycoprotein intracellular transport. Significance and Impact of the Study: Given its antimicrobial activity and its safety for human tissues, subtilosin could represent a valuable alternative to be considered in the development of new microbicide formulations. © 2014 The Society for Applied Microbiology. Fil:Torres, N.I. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Wachsman, M.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Castilla, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2014 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13645072_v117_n5_p1253_Quintana http://hdl.handle.net/20.500.12110/paper_13645072_v117_n5_p1253_Quintana
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Antimicrobial peptide Antiviral activity Bacteriocin Herpes simplex virus Microbicide Subtilosin antivirus agent bacteriocin glycoprotein glycoprotein gd polypeptide antibiotic agent subtilosin unclassified drug antivirus agent bacteriocin cyclopeptide sboA protein, Bacillus subtilis animal cell antiviral activity Article Bacillus amyloliquefaciens cell culture cell viability cellular distribution controlled study cytotoxicity dose response drug mechanism Herpes simplex virus 2 immunofluorescence test intracellular space intracellular transport nonhuman protein expression protein isolation Vero cell line virus particle virus release virus replication Western blotting animal Chlorocebus aethiops drug effects Herpes simplex virus 2 human Simplexvirus Animals Antiviral Agents Bacteriocins Cercopithecus aethiops Herpesvirus 2, Human Humans Peptides, Cyclic Vero Cells
spellingShingle	Antimicrobial peptide Antiviral activity Bacteriocin Herpes simplex virus Microbicide Subtilosin antivirus agent bacteriocin glycoprotein glycoprotein gd polypeptide antibiotic agent subtilosin unclassified drug antivirus agent bacteriocin cyclopeptide sboA protein, Bacillus subtilis animal cell antiviral activity Article Bacillus amyloliquefaciens cell culture cell viability cellular distribution controlled study cytotoxicity dose response drug mechanism Herpes simplex virus 2 immunofluorescence test intracellular space intracellular transport nonhuman protein expression protein isolation Vero cell line virus particle virus release virus replication Western blotting animal Chlorocebus aethiops drug effects Herpes simplex virus 2 human Simplexvirus Animals Antiviral Agents Bacteriocins Cercopithecus aethiops Herpesvirus 2, Human Humans Peptides, Cyclic Vero Cells Torres, Nicolás I. Wachsman, Mónica Beatriz Castilla, Viviana Antiherpes simplex virus type 2 activity of the antimicrobial peptide subtilosin
topic_facet	Antimicrobial peptide Antiviral activity Bacteriocin Herpes simplex virus Microbicide Subtilosin antivirus agent bacteriocin glycoprotein glycoprotein gd polypeptide antibiotic agent subtilosin unclassified drug antivirus agent bacteriocin cyclopeptide sboA protein, Bacillus subtilis animal cell antiviral activity Article Bacillus amyloliquefaciens cell culture cell viability cellular distribution controlled study cytotoxicity dose response drug mechanism Herpes simplex virus 2 immunofluorescence test intracellular space intracellular transport nonhuman protein expression protein isolation Vero cell line virus particle virus release virus replication Western blotting animal Chlorocebus aethiops drug effects Herpes simplex virus 2 human Simplexvirus Animals Antiviral Agents Bacteriocins Cercopithecus aethiops Herpesvirus 2, Human Humans Peptides, Cyclic Vero Cells
description	Aims: In this study, we evaluated the antiviral activity of subtilosin, a cyclical peptide isolated from Bacillus amyloliquefaciens, against herpes simplex virus type 2 (HSV-2) in cell cultures and we investigated subtilosin mode of action. Methods and Results: We determined, using a virus yield inhibition assay, that noncytotoxic concentrations of subtilosin inhibit HSV-2 replication in Vero cell cultures. Subtilosin strongly inhibited extracellular and total virus production even when it was added at 8 h postinfection indicating that not only virus release but also viral particle formation is impeded by the antiviral peptide. Although viral glycoprotein gD level of expression is not affected by the bacteriocin, an altered pattern of gD intracellular localization was detected by immunofluorescence assay in subtilosin-treated culture. On the other hand, at high concentrations, subtilosin displays virucidal action. Conclusions: Subtilosin displays antiviral and virucidal actions against HSV-2. The target of subtilosin inhibitory effect would be late stages of the viral replicative cycle such as viral glycoprotein intracellular transport. Significance and Impact of the Study: Given its antimicrobial activity and its safety for human tissues, subtilosin could represent a valuable alternative to be considered in the development of new microbicide formulations. © 2014 The Society for Applied Microbiology.
author	Torres, Nicolás I. Wachsman, Mónica Beatriz Castilla, Viviana
author_facet	Torres, Nicolás I. Wachsman, Mónica Beatriz Castilla, Viviana
author_sort	Torres, Nicolás I.
title	Antiherpes simplex virus type 2 activity of the antimicrobial peptide subtilosin
title_short	Antiherpes simplex virus type 2 activity of the antimicrobial peptide subtilosin
title_full	Antiherpes simplex virus type 2 activity of the antimicrobial peptide subtilosin
title_fullStr	Antiherpes simplex virus type 2 activity of the antimicrobial peptide subtilosin
title_full_unstemmed	Antiherpes simplex virus type 2 activity of the antimicrobial peptide subtilosin
title_sort	antiherpes simplex virus type 2 activity of the antimicrobial peptide subtilosin
publishDate	2014
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13645072_v117_n5_p1253_Quintana http://hdl.handle.net/20.500.12110/paper_13645072_v117_n5_p1253_Quintana
work_keys_str_mv	AT torresnicolasi antiherpessimplexvirustype2activityoftheantimicrobialpeptidesubtilosin AT wachsmanmonicabeatriz antiherpessimplexvirustype2activityoftheantimicrobialpeptidesubtilosin AT castillaviviana antiherpessimplexvirustype2activityoftheantimicrobialpeptidesubtilosin
_version_	1768545801196273664

Antiherpes simplex virus type 2 activity of the antimicrobial peptide subtilosin

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