Medium pH, carbon and nitrogen concentrations modulate the phosphate solubilization efficiency of Penicillium purpurogenum through organic acid production

Aims: To study phosphate solubilization in Penicillium purpurogenum as function of medium pH, and carbon and nitrogen concentrations. Methods and Results: Tricalcium phosphate (CP) solubilization efficiency of P. purpurogenum was evaluated at acid or alkaline pH using different C and N sources. Gluc...

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Autores principales:	Scervino, José Martín, Papinutti, Víctor Leandro, Rodríguez, María Alejandra, Della Mónica, Ivana Florencia, Pettinari, María Julia, Godeas, Alicia Margarita
Publicado:	2011
Materias:	Organic acids Penicillium purpurogenum Phosphate-solubilizing micro-organisms Phosphorus ammonium sulfate calcium carbon carboxylic acid fructose gluconic acid glucose nitrogen phosphate alkalinity concentration (composition) experimental design fertilizer application fungus inoculation optimization organic acid pH soil ecosystem soil microorganism solubilization article bioprocess ecosystem fungus culture fungus growth nonhuman Calcium Phosphates Carbon Culture Media Gluconates Hydrogen-Ion Concentration Nitrogen Penicillium Soil Microbiology Solubility
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13645072_v110_n5_p1215_Scervino http://hdl.handle.net/20.500.12110/paper_13645072_v110_n5_p1215_Scervino
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_13645072_v110_n5_p1215_Scervino
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spelling	paper:paper_13645072_v110_n5_p1215_Scervino2023-06-08T16:11:44Z Medium pH, carbon and nitrogen concentrations modulate the phosphate solubilization efficiency of Penicillium purpurogenum through organic acid production Scervino, José Martín Papinutti, Víctor Leandro Rodríguez, María Alejandra Della Mónica, Ivana Florencia Pettinari, María Julia Godeas, Alicia Margarita Organic acids Penicillium purpurogenum Phosphate-solubilizing micro-organisms Phosphorus ammonium sulfate calcium carbon carboxylic acid fructose gluconic acid glucose nitrogen phosphate alkalinity calcium carbon concentration (composition) experimental design fertilizer application fungus glucose inoculation nitrogen optimization organic acid pH phosphate soil ecosystem soil microorganism solubilization alkalinity article bioprocess ecosystem fungus culture fungus growth inoculation nonhuman Penicillium purpurogenum pH solubilization Calcium Phosphates Carbon Culture Media Gluconates Hydrogen-Ion Concentration Nitrogen Penicillium Soil Microbiology Solubility Penicillium purpurogenum Aims: To study phosphate solubilization in Penicillium purpurogenum as function of medium pH, and carbon and nitrogen concentrations. Methods and Results: Tricalcium phosphate (CP) solubilization efficiency of P. purpurogenum was evaluated at acid or alkaline pH using different C and N sources. Glucose- and (NH4)2SO4-based media showed the highest P solubilization values followed by fructose. P. purpurogenum solubilizing ability was higher in cultures grown at pH 6·5 than cultures at pH 8·5. Organic acids were detected in both alkaline and neutral media, but the relative percentages of each organic acid differed. Highest P release coincided with the highest organic acids production peak, especially gluconic acid. When P. purpurogenum grew in alkaline media, the nature and concentration of organic acids changed at different N and C concentrations. A factorial categorical experimental design showed that the highest P-solubilizing activity, coinciding with the highest organic acid production, corresponded to the highest C concentration and lowest N concentration. Conclusions: The results described in the present study show that medium pH and carbon and nitrogen concentrations modulate the P solubilization efficiency of P. purpurogenum through the production of organic acids and particularly that of gluconic acid. In the P solubilization optimization studies, glucose and (NH4)2SO4 as C and N sources allowed a higher solubilization efficiency at high pH. Significance and Impact of the Study: This organism is a potentially proficient soil inoculant, especially in P-poor alkaline soils where other P solubilizers fail to release soluble P. Further work is necessary to elucidate whether these results can be extrapolated to natural soil ecosystems, where different pH values are present. Penicillium purpurogenum could be used to develop a bioprocess for the manufacture of phosphatic fertilizer with phosphate calcium minerals. © 2011 The Authors. Journal of Applied Microbiology © 2011 The Society for Applied Microbiology. Fil:Scervino, J.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Papinutti, V.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rodriguez, M.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Della Monica, I. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pettinari, M.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Godeas, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2011 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13645072_v110_n5_p1215_Scervino http://hdl.handle.net/20.500.12110/paper_13645072_v110_n5_p1215_Scervino
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Organic acids Penicillium purpurogenum Phosphate-solubilizing micro-organisms Phosphorus ammonium sulfate calcium carbon carboxylic acid fructose gluconic acid glucose nitrogen phosphate alkalinity calcium carbon concentration (composition) experimental design fertilizer application fungus glucose inoculation nitrogen optimization organic acid pH phosphate soil ecosystem soil microorganism solubilization alkalinity article bioprocess ecosystem fungus culture fungus growth inoculation nonhuman Penicillium purpurogenum pH solubilization Calcium Phosphates Carbon Culture Media Gluconates Hydrogen-Ion Concentration Nitrogen Penicillium Soil Microbiology Solubility Penicillium purpurogenum
spellingShingle	Organic acids Penicillium purpurogenum Phosphate-solubilizing micro-organisms Phosphorus ammonium sulfate calcium carbon carboxylic acid fructose gluconic acid glucose nitrogen phosphate alkalinity calcium carbon concentration (composition) experimental design fertilizer application fungus glucose inoculation nitrogen optimization organic acid pH phosphate soil ecosystem soil microorganism solubilization alkalinity article bioprocess ecosystem fungus culture fungus growth inoculation nonhuman Penicillium purpurogenum pH solubilization Calcium Phosphates Carbon Culture Media Gluconates Hydrogen-Ion Concentration Nitrogen Penicillium Soil Microbiology Solubility Penicillium purpurogenum Scervino, José Martín Papinutti, Víctor Leandro Rodríguez, María Alejandra Della Mónica, Ivana Florencia Pettinari, María Julia Godeas, Alicia Margarita Medium pH, carbon and nitrogen concentrations modulate the phosphate solubilization efficiency of Penicillium purpurogenum through organic acid production
topic_facet	Organic acids Penicillium purpurogenum Phosphate-solubilizing micro-organisms Phosphorus ammonium sulfate calcium carbon carboxylic acid fructose gluconic acid glucose nitrogen phosphate alkalinity calcium carbon concentration (composition) experimental design fertilizer application fungus glucose inoculation nitrogen optimization organic acid pH phosphate soil ecosystem soil microorganism solubilization alkalinity article bioprocess ecosystem fungus culture fungus growth inoculation nonhuman Penicillium purpurogenum pH solubilization Calcium Phosphates Carbon Culture Media Gluconates Hydrogen-Ion Concentration Nitrogen Penicillium Soil Microbiology Solubility Penicillium purpurogenum
description	Aims: To study phosphate solubilization in Penicillium purpurogenum as function of medium pH, and carbon and nitrogen concentrations. Methods and Results: Tricalcium phosphate (CP) solubilization efficiency of P. purpurogenum was evaluated at acid or alkaline pH using different C and N sources. Glucose- and (NH4)2SO4-based media showed the highest P solubilization values followed by fructose. P. purpurogenum solubilizing ability was higher in cultures grown at pH 6·5 than cultures at pH 8·5. Organic acids were detected in both alkaline and neutral media, but the relative percentages of each organic acid differed. Highest P release coincided with the highest organic acids production peak, especially gluconic acid. When P. purpurogenum grew in alkaline media, the nature and concentration of organic acids changed at different N and C concentrations. A factorial categorical experimental design showed that the highest P-solubilizing activity, coinciding with the highest organic acid production, corresponded to the highest C concentration and lowest N concentration. Conclusions: The results described in the present study show that medium pH and carbon and nitrogen concentrations modulate the P solubilization efficiency of P. purpurogenum through the production of organic acids and particularly that of gluconic acid. In the P solubilization optimization studies, glucose and (NH4)2SO4 as C and N sources allowed a higher solubilization efficiency at high pH. Significance and Impact of the Study: This organism is a potentially proficient soil inoculant, especially in P-poor alkaline soils where other P solubilizers fail to release soluble P. Further work is necessary to elucidate whether these results can be extrapolated to natural soil ecosystems, where different pH values are present. Penicillium purpurogenum could be used to develop a bioprocess for the manufacture of phosphatic fertilizer with phosphate calcium minerals. © 2011 The Authors. Journal of Applied Microbiology © 2011 The Society for Applied Microbiology.
author	Scervino, José Martín Papinutti, Víctor Leandro Rodríguez, María Alejandra Della Mónica, Ivana Florencia Pettinari, María Julia Godeas, Alicia Margarita
author_facet	Scervino, José Martín Papinutti, Víctor Leandro Rodríguez, María Alejandra Della Mónica, Ivana Florencia Pettinari, María Julia Godeas, Alicia Margarita
author_sort	Scervino, José Martín
title	Medium pH, carbon and nitrogen concentrations modulate the phosphate solubilization efficiency of Penicillium purpurogenum through organic acid production
title_short	Medium pH, carbon and nitrogen concentrations modulate the phosphate solubilization efficiency of Penicillium purpurogenum through organic acid production
title_full	Medium pH, carbon and nitrogen concentrations modulate the phosphate solubilization efficiency of Penicillium purpurogenum through organic acid production
title_fullStr	Medium pH, carbon and nitrogen concentrations modulate the phosphate solubilization efficiency of Penicillium purpurogenum through organic acid production
title_full_unstemmed	Medium pH, carbon and nitrogen concentrations modulate the phosphate solubilization efficiency of Penicillium purpurogenum through organic acid production
title_sort	medium ph, carbon and nitrogen concentrations modulate the phosphate solubilization efficiency of penicillium purpurogenum through organic acid production
publishDate	2011
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13645072_v110_n5_p1215_Scervino http://hdl.handle.net/20.500.12110/paper_13645072_v110_n5_p1215_Scervino
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Medium pH, carbon and nitrogen concentrations modulate the phosphate solubilization efficiency of Penicillium purpurogenum through organic acid production

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