Human testicular protein TPX1/CRISP-2: Localization in spermatozoa, fate after capacitation and relevance for gamete interaction
Testicular protein Tpx-1, also known as CRISP-2, is a cysteine-rich secretory protein specifically expressed in the male reproductive tract. Since the information available on the human protein is limited to the identification and expression of its gene, in this work we have studied the presence and...
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2005
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13609947_v11_n4_p299_Busso http://hdl.handle.net/20.500.12110/paper_13609947_v11_n4_p299_Busso |
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paper:paper_13609947_v11_n4_p299_Busso2023-06-08T16:11:35Z Human testicular protein TPX1/CRISP-2: Localization in spermatozoa, fate after capacitation and relevance for gamete interaction Acrosome reaction CRISP family Gamete interaction Sperm Testes cysteine ionophore secretory protein testicular protein unclassified drug acrosome acrosome reaction adult animal cell article binding kinetics cell fate cell interaction cell labeling controlled study cytochemistry electrophoretic mobility female gamete hamster human human cell immunofluorescence in vitro study incubation time inhibition kinetics male male genital system membrane permeability nonhuman oocyte priority journal protein analysis protein degradation protein expression protein localization protein modification semen analysis spermatozoon spermatozoon capacitation spermatozoon motility spermatozoon penetration zona pellucida Antibodies Glycoproteins Humans Male Sperm Capacitation Sperm-Ovum Interactions Spermatozoa Cricetinae Testicular protein Tpx-1, also known as CRISP-2, is a cysteine-rich secretory protein specifically expressed in the male reproductive tract. Since the information available on the human protein is limited to the identification and expression of its gene, in this work we have studied the presence and localization of human Tpx-1 (TPX1) in sperm, its fate after capacitation and acrosome reaction (AR), and its possible involvement in gamete interaction. Indirect immunofluorescence studies revealed the absence of significant staining in live or fixed non-permeabilized sperm, in contrast to a clear labelling in the acrosomal region of permeabilized sperm. These results, together with complementary evidence from protein extraction procedures strongly support that TPX1 would he mainly an intra-acrosomal protein in fresh sperm. After in vitro capacitation and ionophore-induced AR, TPX1 remained associated with the equatorial segment of the acrosome. The lack of differences in the electrophoretic mobility of TPX1 before and after capacitation and AR indicates that the protein would not undergo proteolytical modifications during these processes. The possible involvement of TPX1 in gamete interaction was evaluated by the hamster oocyte penetration test. The presence of anti-TPX1 during gamete co-incubation produced a significant and dose-dependent inhibition in the percentage of penetrated zona-free hamster oocytes without affecting sperm motility, the AR or sperm binding to the oolema. Together, these results indicate that human TPX1 would be a component of the sperm acrosome that remains associated with sperm after capacitation and AR, and is relevant for sperm-oocyte interaction. © European Society of Human Reproduction and Embryology 2005; all rights reserved. 2005 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13609947_v11_n4_p299_Busso http://hdl.handle.net/20.500.12110/paper_13609947_v11_n4_p299_Busso |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Acrosome reaction CRISP family Gamete interaction Sperm Testes cysteine ionophore secretory protein testicular protein unclassified drug acrosome acrosome reaction adult animal cell article binding kinetics cell fate cell interaction cell labeling controlled study cytochemistry electrophoretic mobility female gamete hamster human human cell immunofluorescence in vitro study incubation time inhibition kinetics male male genital system membrane permeability nonhuman oocyte priority journal protein analysis protein degradation protein expression protein localization protein modification semen analysis spermatozoon spermatozoon capacitation spermatozoon motility spermatozoon penetration zona pellucida Antibodies Glycoproteins Humans Male Sperm Capacitation Sperm-Ovum Interactions Spermatozoa Cricetinae |
| spellingShingle |
Acrosome reaction CRISP family Gamete interaction Sperm Testes cysteine ionophore secretory protein testicular protein unclassified drug acrosome acrosome reaction adult animal cell article binding kinetics cell fate cell interaction cell labeling controlled study cytochemistry electrophoretic mobility female gamete hamster human human cell immunofluorescence in vitro study incubation time inhibition kinetics male male genital system membrane permeability nonhuman oocyte priority journal protein analysis protein degradation protein expression protein localization protein modification semen analysis spermatozoon spermatozoon capacitation spermatozoon motility spermatozoon penetration zona pellucida Antibodies Glycoproteins Humans Male Sperm Capacitation Sperm-Ovum Interactions Spermatozoa Cricetinae Human testicular protein TPX1/CRISP-2: Localization in spermatozoa, fate after capacitation and relevance for gamete interaction |
| topic_facet |
Acrosome reaction CRISP family Gamete interaction Sperm Testes cysteine ionophore secretory protein testicular protein unclassified drug acrosome acrosome reaction adult animal cell article binding kinetics cell fate cell interaction cell labeling controlled study cytochemistry electrophoretic mobility female gamete hamster human human cell immunofluorescence in vitro study incubation time inhibition kinetics male male genital system membrane permeability nonhuman oocyte priority journal protein analysis protein degradation protein expression protein localization protein modification semen analysis spermatozoon spermatozoon capacitation spermatozoon motility spermatozoon penetration zona pellucida Antibodies Glycoproteins Humans Male Sperm Capacitation Sperm-Ovum Interactions Spermatozoa Cricetinae |
| description |
Testicular protein Tpx-1, also known as CRISP-2, is a cysteine-rich secretory protein specifically expressed in the male reproductive tract. Since the information available on the human protein is limited to the identification and expression of its gene, in this work we have studied the presence and localization of human Tpx-1 (TPX1) in sperm, its fate after capacitation and acrosome reaction (AR), and its possible involvement in gamete interaction. Indirect immunofluorescence studies revealed the absence of significant staining in live or fixed non-permeabilized sperm, in contrast to a clear labelling in the acrosomal region of permeabilized sperm. These results, together with complementary evidence from protein extraction procedures strongly support that TPX1 would he mainly an intra-acrosomal protein in fresh sperm. After in vitro capacitation and ionophore-induced AR, TPX1 remained associated with the equatorial segment of the acrosome. The lack of differences in the electrophoretic mobility of TPX1 before and after capacitation and AR indicates that the protein would not undergo proteolytical modifications during these processes. The possible involvement of TPX1 in gamete interaction was evaluated by the hamster oocyte penetration test. The presence of anti-TPX1 during gamete co-incubation produced a significant and dose-dependent inhibition in the percentage of penetrated zona-free hamster oocytes without affecting sperm motility, the AR or sperm binding to the oolema. Together, these results indicate that human TPX1 would be a component of the sperm acrosome that remains associated with sperm after capacitation and AR, and is relevant for sperm-oocyte interaction. © European Society of Human Reproduction and Embryology 2005; all rights reserved. |
| title |
Human testicular protein TPX1/CRISP-2: Localization in spermatozoa, fate after capacitation and relevance for gamete interaction |
| title_short |
Human testicular protein TPX1/CRISP-2: Localization in spermatozoa, fate after capacitation and relevance for gamete interaction |
| title_full |
Human testicular protein TPX1/CRISP-2: Localization in spermatozoa, fate after capacitation and relevance for gamete interaction |
| title_fullStr |
Human testicular protein TPX1/CRISP-2: Localization in spermatozoa, fate after capacitation and relevance for gamete interaction |
| title_full_unstemmed |
Human testicular protein TPX1/CRISP-2: Localization in spermatozoa, fate after capacitation and relevance for gamete interaction |
| title_sort |
human testicular protein tpx1/crisp-2: localization in spermatozoa, fate after capacitation and relevance for gamete interaction |
| publishDate |
2005 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13609947_v11_n4_p299_Busso http://hdl.handle.net/20.500.12110/paper_13609947_v11_n4_p299_Busso |
| _version_ |
1768546505416769536 |