Cellular inactivation and antitumor efficacy of a new zinc phthalocyanine with potential use in photodynamic therapy
The aim of the present study was to evaluate the photodynamic efficacy of a novel phthalocyanine derivate 2,3,9,10,16,17,23,24-octakis[(N,N-dimethylamino) ethylsulfanyl]phthalocyaninatozinc(II) (referred here as S1) using MCF-7c3 human breast cancer cells and the LM2 adenocarcinoma subcutaneously im...
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2008
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13572725_v40_n10_p2192_Vittar http://hdl.handle.net/20.500.12110/paper_13572725_v40_n10_p2192_Vittar |
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paper:paper_13572725_v40_n10_p2192_Vittar2023-06-08T16:11:18Z Cellular inactivation and antitumor efficacy of a new zinc phthalocyanine with potential use in photodynamic therapy Apoptosis Cancer Necrosis Photodynamic Therapy Photosensitizer dimyristoylphosphatidylcholine liposome phthalocyanine zinc adenocarcinoma animal cell animal model animal tissue antineoplastic activity apoptosis article breast cancer cancer cell culture cancer inhibition cell activation cell damage controlled study cytolysis drug delivery system drug distribution female human human cell incubation time internalization light damage light irradiance lysosome microscopy mitochondrion morphology mouse nonhuman photodynamic therapy photodynamics treatment response tumor volume Animals Cell Death Cell Line, Tumor Cell Survival Culture Media Darkness Female Humans Indoles Intracellular Space Mice Mice, Inbred BALB C Neoplasms Organometallic Compounds Photochemotherapy Solutions Spectrometry, Fluorescence Treatment Outcome Xenograft Model Antitumor Assays Animalia Mus The aim of the present study was to evaluate the photodynamic efficacy of a novel phthalocyanine derivate 2,3,9,10,16,17,23,24-octakis[(N,N-dimethylamino) ethylsulfanyl]phthalocyaninatozinc(II) (referred here as S1) using MCF-7c3 human breast cancer cells and the LM2 adenocarcinoma subcutaneously implanted in Balb/c mice as experimental models. The S1-l-α-dimyristoyl-phosphatidylcholine liposome was selected as the best delivery system because it showed greater internalization into cells (35 nmol/106 cells), relative to other liposomes. After 3 h incubation S1 was partially localized in lysosomes, the compartment that represented its primary photodamage site. The S1 treated cultures also revealed a degree of mitochondrial morphology alteration. Indeed, S1 leads to photokilling of the cells with different efficacies indicating that cell photoinactivation was dependent on both the phthalocyanine concentration and the light dose applied. Analyses of morphology and nuclear condensation level indicated that some of the cells exposed to photodynamic therapy were undergoing apoptosis within 8 h after treatment. To assess the in vivo effectiveness of S1, animals bearing tumors were treated with 0.2 mg/kg S1 followed 24 h later by 108 J cm-2 light at 600-800 nm and 60 mW cm-2,while other animals served as controls (no treatment, light alone, or S1 alone). All S1 treated tumors and none of the controls exhibited complete or partial responses, and these responses continued for the entire observation period of 12 days. Evaluation of tumor size showed that the treatment effectively delayed tumor growth. Light microscopy investigations of irradiated tumor specimens showed that S1 causes an early direct damage of malignant cells, largely via processes leading to random necrotic pathways. © 2008 Elsevier Ltd. All rights reserved. 2008 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13572725_v40_n10_p2192_Vittar http://hdl.handle.net/20.500.12110/paper_13572725_v40_n10_p2192_Vittar |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Apoptosis Cancer Necrosis Photodynamic Therapy Photosensitizer dimyristoylphosphatidylcholine liposome phthalocyanine zinc adenocarcinoma animal cell animal model animal tissue antineoplastic activity apoptosis article breast cancer cancer cell culture cancer inhibition cell activation cell damage controlled study cytolysis drug delivery system drug distribution female human human cell incubation time internalization light damage light irradiance lysosome microscopy mitochondrion morphology mouse nonhuman photodynamic therapy photodynamics treatment response tumor volume Animals Cell Death Cell Line, Tumor Cell Survival Culture Media Darkness Female Humans Indoles Intracellular Space Mice Mice, Inbred BALB C Neoplasms Organometallic Compounds Photochemotherapy Solutions Spectrometry, Fluorescence Treatment Outcome Xenograft Model Antitumor Assays Animalia Mus |
| spellingShingle |
Apoptosis Cancer Necrosis Photodynamic Therapy Photosensitizer dimyristoylphosphatidylcholine liposome phthalocyanine zinc adenocarcinoma animal cell animal model animal tissue antineoplastic activity apoptosis article breast cancer cancer cell culture cancer inhibition cell activation cell damage controlled study cytolysis drug delivery system drug distribution female human human cell incubation time internalization light damage light irradiance lysosome microscopy mitochondrion morphology mouse nonhuman photodynamic therapy photodynamics treatment response tumor volume Animals Cell Death Cell Line, Tumor Cell Survival Culture Media Darkness Female Humans Indoles Intracellular Space Mice Mice, Inbred BALB C Neoplasms Organometallic Compounds Photochemotherapy Solutions Spectrometry, Fluorescence Treatment Outcome Xenograft Model Antitumor Assays Animalia Mus Cellular inactivation and antitumor efficacy of a new zinc phthalocyanine with potential use in photodynamic therapy |
| topic_facet |
Apoptosis Cancer Necrosis Photodynamic Therapy Photosensitizer dimyristoylphosphatidylcholine liposome phthalocyanine zinc adenocarcinoma animal cell animal model animal tissue antineoplastic activity apoptosis article breast cancer cancer cell culture cancer inhibition cell activation cell damage controlled study cytolysis drug delivery system drug distribution female human human cell incubation time internalization light damage light irradiance lysosome microscopy mitochondrion morphology mouse nonhuman photodynamic therapy photodynamics treatment response tumor volume Animals Cell Death Cell Line, Tumor Cell Survival Culture Media Darkness Female Humans Indoles Intracellular Space Mice Mice, Inbred BALB C Neoplasms Organometallic Compounds Photochemotherapy Solutions Spectrometry, Fluorescence Treatment Outcome Xenograft Model Antitumor Assays Animalia Mus |
| description |
The aim of the present study was to evaluate the photodynamic efficacy of a novel phthalocyanine derivate 2,3,9,10,16,17,23,24-octakis[(N,N-dimethylamino) ethylsulfanyl]phthalocyaninatozinc(II) (referred here as S1) using MCF-7c3 human breast cancer cells and the LM2 adenocarcinoma subcutaneously implanted in Balb/c mice as experimental models. The S1-l-α-dimyristoyl-phosphatidylcholine liposome was selected as the best delivery system because it showed greater internalization into cells (35 nmol/106 cells), relative to other liposomes. After 3 h incubation S1 was partially localized in lysosomes, the compartment that represented its primary photodamage site. The S1 treated cultures also revealed a degree of mitochondrial morphology alteration. Indeed, S1 leads to photokilling of the cells with different efficacies indicating that cell photoinactivation was dependent on both the phthalocyanine concentration and the light dose applied. Analyses of morphology and nuclear condensation level indicated that some of the cells exposed to photodynamic therapy were undergoing apoptosis within 8 h after treatment. To assess the in vivo effectiveness of S1, animals bearing tumors were treated with 0.2 mg/kg S1 followed 24 h later by 108 J cm-2 light at 600-800 nm and 60 mW cm-2,while other animals served as controls (no treatment, light alone, or S1 alone). All S1 treated tumors and none of the controls exhibited complete or partial responses, and these responses continued for the entire observation period of 12 days. Evaluation of tumor size showed that the treatment effectively delayed tumor growth. Light microscopy investigations of irradiated tumor specimens showed that S1 causes an early direct damage of malignant cells, largely via processes leading to random necrotic pathways. © 2008 Elsevier Ltd. All rights reserved. |
| title |
Cellular inactivation and antitumor efficacy of a new zinc phthalocyanine with potential use in photodynamic therapy |
| title_short |
Cellular inactivation and antitumor efficacy of a new zinc phthalocyanine with potential use in photodynamic therapy |
| title_full |
Cellular inactivation and antitumor efficacy of a new zinc phthalocyanine with potential use in photodynamic therapy |
| title_fullStr |
Cellular inactivation and antitumor efficacy of a new zinc phthalocyanine with potential use in photodynamic therapy |
| title_full_unstemmed |
Cellular inactivation and antitumor efficacy of a new zinc phthalocyanine with potential use in photodynamic therapy |
| title_sort |
cellular inactivation and antitumor efficacy of a new zinc phthalocyanine with potential use in photodynamic therapy |
| publishDate |
2008 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13572725_v40_n10_p2192_Vittar http://hdl.handle.net/20.500.12110/paper_13572725_v40_n10_p2192_Vittar |
| _version_ |
1768544005700714496 |