On the promoting action of tamoxifen in a model of hepatocarcinogenesis induced by p-dimethylaminoazobenzene in CF1 mice
Background and aims: Tamoxifen (TMX) has proven to be an effective palliative treatment for advanced breast cancer with low reported incidence of side effects. TMX has been demonstrated to be an initiator and/or a promoter in the rat model of hepatocarcinogenesis. To document the long-term effect of...
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2001
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13572725_v33_n7_p681_Caballero http://hdl.handle.net/20.500.12110/paper_13572725_v33_n7_p681_Caballero |
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paper:paper_13572725_v33_n7_p681_Caballero2023-06-08T16:11:16Z On the promoting action of tamoxifen in a model of hepatocarcinogenesis induced by p-dimethylaminoazobenzene in CF1 mice Gerez, Esther Noemí Batlle, Alcira María del Carmen Vázquez, Elba Susana Cytochrome P450 Hepatocarcinogenesis Oxidative stress P-Dimethylaminoazobenzene Tamoxifen 4 dimethylaminoazobenzene antineoplastic agent catalase cytochrome P450 glutathione transferase liver enzyme superoxide dismutase tamoxifen tamoxifen citrate animal experiment animal model animal tissue article controlled study degradation diet enzyme activity heme synthesis liver carcinogenesis liver cell carcinoma liver hyperplasia liver injury liver tumor long term care male mouse nonhuman oxidative stress response time 5-Aminolevulinate Synthetase Animals Body Weight Carcinogens Catalase Cytochrome P-450 Enzyme System Glutathione Transferase Heme Oxygenase (Decyclizing) Liver Liver Neoplasms, Experimental Male Mice Organ Size p-Dimethylaminoazobenzene Superoxide Dismutase Tamoxifen Thiobarbituric Acid Reactive Substances Time Factors Animalia Background and aims: Tamoxifen (TMX) has proven to be an effective palliative treatment for advanced breast cancer with low reported incidence of side effects. TMX has been demonstrated to be an initiator and/or a promoter in the rat model of hepatocarcinogenesis. To document the long-term effect of TMX in mice treated with p-dimethylaminoazobenzene (DAB), we have investigated the time response action of these drugs on different biochemical parameters. Methods: A group of animals was placed on dietary DAB (0.5%, w/w) during a period of 28 weeks. Control animals received a standard laboratory diet. Two other groups of non-treated and DAB-treated animals received TMX citrate (0.025%, w/w) in the diet since day 20. Results: The activities of the enzymes involved in heme synthesis and degradation as evaluated in the DAB group was not further affected by TMX. DAB and/or TMX treatment significantly increased the content of total cytochrome P450 and also the activity of glutathione S-transferase indicating liver damage. In all treated groups oxidative stress and an adaptive response of the natural defense system (catalase and superoxide dismutase) were demonstrated. Histological and morphological studies revealed liver cell hyperplasia in DAB treated group; however, only in the DAB+TMX group solid, trabecular and acinar hepatocellular carcinoma was confirmed at the end of the experimental trial. Conclusion: We have demonstrated that TMX produced changes in hepatic enzyme activities which may be relevant for the metabolism and disposition of this and/or other drugs. Because liver tumors could be initiated and promoted by several agents which need to be activated, the possible hazard of TMX should be considered. This study reports that long-term treatment with TMX enhances hepatocarcinogenesis induced by DAB. The widespread use of TMX as an anticancer agent adds to the significance of this study. © 2001 Published by Elsevier Science Ltd. Fil:Gerez, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Batlle, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Vazquez, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2001 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13572725_v33_n7_p681_Caballero http://hdl.handle.net/20.500.12110/paper_13572725_v33_n7_p681_Caballero |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Cytochrome P450 Hepatocarcinogenesis Oxidative stress P-Dimethylaminoazobenzene Tamoxifen 4 dimethylaminoazobenzene antineoplastic agent catalase cytochrome P450 glutathione transferase liver enzyme superoxide dismutase tamoxifen tamoxifen citrate animal experiment animal model animal tissue article controlled study degradation diet enzyme activity heme synthesis liver carcinogenesis liver cell carcinoma liver hyperplasia liver injury liver tumor long term care male mouse nonhuman oxidative stress response time 5-Aminolevulinate Synthetase Animals Body Weight Carcinogens Catalase Cytochrome P-450 Enzyme System Glutathione Transferase Heme Oxygenase (Decyclizing) Liver Liver Neoplasms, Experimental Male Mice Organ Size p-Dimethylaminoazobenzene Superoxide Dismutase Tamoxifen Thiobarbituric Acid Reactive Substances Time Factors Animalia |
| spellingShingle |
Cytochrome P450 Hepatocarcinogenesis Oxidative stress P-Dimethylaminoazobenzene Tamoxifen 4 dimethylaminoazobenzene antineoplastic agent catalase cytochrome P450 glutathione transferase liver enzyme superoxide dismutase tamoxifen tamoxifen citrate animal experiment animal model animal tissue article controlled study degradation diet enzyme activity heme synthesis liver carcinogenesis liver cell carcinoma liver hyperplasia liver injury liver tumor long term care male mouse nonhuman oxidative stress response time 5-Aminolevulinate Synthetase Animals Body Weight Carcinogens Catalase Cytochrome P-450 Enzyme System Glutathione Transferase Heme Oxygenase (Decyclizing) Liver Liver Neoplasms, Experimental Male Mice Organ Size p-Dimethylaminoazobenzene Superoxide Dismutase Tamoxifen Thiobarbituric Acid Reactive Substances Time Factors Animalia Gerez, Esther Noemí Batlle, Alcira María del Carmen Vázquez, Elba Susana On the promoting action of tamoxifen in a model of hepatocarcinogenesis induced by p-dimethylaminoazobenzene in CF1 mice |
| topic_facet |
Cytochrome P450 Hepatocarcinogenesis Oxidative stress P-Dimethylaminoazobenzene Tamoxifen 4 dimethylaminoazobenzene antineoplastic agent catalase cytochrome P450 glutathione transferase liver enzyme superoxide dismutase tamoxifen tamoxifen citrate animal experiment animal model animal tissue article controlled study degradation diet enzyme activity heme synthesis liver carcinogenesis liver cell carcinoma liver hyperplasia liver injury liver tumor long term care male mouse nonhuman oxidative stress response time 5-Aminolevulinate Synthetase Animals Body Weight Carcinogens Catalase Cytochrome P-450 Enzyme System Glutathione Transferase Heme Oxygenase (Decyclizing) Liver Liver Neoplasms, Experimental Male Mice Organ Size p-Dimethylaminoazobenzene Superoxide Dismutase Tamoxifen Thiobarbituric Acid Reactive Substances Time Factors Animalia |
| description |
Background and aims: Tamoxifen (TMX) has proven to be an effective palliative treatment for advanced breast cancer with low reported incidence of side effects. TMX has been demonstrated to be an initiator and/or a promoter in the rat model of hepatocarcinogenesis. To document the long-term effect of TMX in mice treated with p-dimethylaminoazobenzene (DAB), we have investigated the time response action of these drugs on different biochemical parameters. Methods: A group of animals was placed on dietary DAB (0.5%, w/w) during a period of 28 weeks. Control animals received a standard laboratory diet. Two other groups of non-treated and DAB-treated animals received TMX citrate (0.025%, w/w) in the diet since day 20. Results: The activities of the enzymes involved in heme synthesis and degradation as evaluated in the DAB group was not further affected by TMX. DAB and/or TMX treatment significantly increased the content of total cytochrome P450 and also the activity of glutathione S-transferase indicating liver damage. In all treated groups oxidative stress and an adaptive response of the natural defense system (catalase and superoxide dismutase) were demonstrated. Histological and morphological studies revealed liver cell hyperplasia in DAB treated group; however, only in the DAB+TMX group solid, trabecular and acinar hepatocellular carcinoma was confirmed at the end of the experimental trial. Conclusion: We have demonstrated that TMX produced changes in hepatic enzyme activities which may be relevant for the metabolism and disposition of this and/or other drugs. Because liver tumors could be initiated and promoted by several agents which need to be activated, the possible hazard of TMX should be considered. This study reports that long-term treatment with TMX enhances hepatocarcinogenesis induced by DAB. The widespread use of TMX as an anticancer agent adds to the significance of this study. © 2001 Published by Elsevier Science Ltd. |
| author |
Gerez, Esther Noemí Batlle, Alcira María del Carmen Vázquez, Elba Susana |
| author_facet |
Gerez, Esther Noemí Batlle, Alcira María del Carmen Vázquez, Elba Susana |
| author_sort |
Gerez, Esther Noemí |
| title |
On the promoting action of tamoxifen in a model of hepatocarcinogenesis induced by p-dimethylaminoazobenzene in CF1 mice |
| title_short |
On the promoting action of tamoxifen in a model of hepatocarcinogenesis induced by p-dimethylaminoazobenzene in CF1 mice |
| title_full |
On the promoting action of tamoxifen in a model of hepatocarcinogenesis induced by p-dimethylaminoazobenzene in CF1 mice |
| title_fullStr |
On the promoting action of tamoxifen in a model of hepatocarcinogenesis induced by p-dimethylaminoazobenzene in CF1 mice |
| title_full_unstemmed |
On the promoting action of tamoxifen in a model of hepatocarcinogenesis induced by p-dimethylaminoazobenzene in CF1 mice |
| title_sort |
on the promoting action of tamoxifen in a model of hepatocarcinogenesis induced by p-dimethylaminoazobenzene in cf1 mice |
| publishDate |
2001 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13572725_v33_n7_p681_Caballero http://hdl.handle.net/20.500.12110/paper_13572725_v33_n7_p681_Caballero |
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AT gerezesthernoemi onthepromotingactionoftamoxifeninamodelofhepatocarcinogenesisinducedbypdimethylaminoazobenzeneincf1mice AT batllealciramariadelcarmen onthepromotingactionoftamoxifeninamodelofhepatocarcinogenesisinducedbypdimethylaminoazobenzeneincf1mice AT vazquezelbasusana onthepromotingactionoftamoxifeninamodelofhepatocarcinogenesisinducedbypdimethylaminoazobenzeneincf1mice |
| _version_ |
1768544332787220480 |