UGA4 gene encoding the γ-aminobutyric acid permease in Saccharomyces cerevisiae is an acid-expressed gene
Background and aims: Biological processes in all organisms are controlled by environmental conditions, however, information concerning the molecular responses to external pH is scarce. In this work we studied the pH response of UGA4 gene encoding δ-aminolevulinic acid and γ-aminobutyric acid permeas...
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2001
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13572725_v33_n12_p1202_Moretti http://hdl.handle.net/20.500.12110/paper_13572725_v33_n12_p1202_Moretti |
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paper:paper_13572725_v33_n12_p1202_Moretti2023-06-08T16:11:16Z UGA4 gene encoding the γ-aminobutyric acid permease in Saccharomyces cerevisiae is an acid-expressed gene Batlle, Alcira María del Carmen γ-aminobutyric acid δ-aminolevulinic acid Ambient pH Saccharomyces cerevisiae Transcriptional regulation UGA4 gene 4 aminobutyric acid acid aminolevulinic acid bacterial enzyme beta galactosidase permease protein derivative protein Rim101p transcription factor unclassified drug acidity article binding site consensus sequence controlled study enzyme activity fusion gene gene expression genetic code nonhuman pH protein induction repressor gene Saccharomyces cerevisiae transcription regulation Aminolevulinic Acid beta-Galactosidase Binding Sites GABA Plasma Membrane Transport Proteins Hydrogen-Ion Concentration Lac Operon Organic Anion Transporters Promoter Regions (Genetics) Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Saccharomyces cerevisiae Background and aims: Biological processes in all organisms are controlled by environmental conditions, however, information concerning the molecular responses to external pH is scarce. In this work we studied the pH response of UGA4 gene encoding δ-aminolevulinic acid and γ-aminobutyric acid permease in Saccharomyces cerevisiae. Methods: We analyzed the effect of pH on the expression of UGA4 gene measuring β-galactosidase activity in cells carrying a UGA4::lacZ fusion gene. Results: Results indicate that UGA4 expression is higher at acidic pH. The expression of UGA3 and UGA35 genes, which encode two positive transcription factors, is not regulated by external pH, while the expression of UGA43 gene encoding a repressor of UGA4 transcription is dependent on pH. Using a strain lacking Uga43p we clearly showed that the effect of ambient pH on UGA4 expression is not a secondary effect of the pH regulation on UGA43. We have also demonstrated that the effect of pH can only be detected when UGA4 gene is not subject to a strong repression by Uga43p nor to GABA induction. Conclusion: Here, we demonstrate that UGA4 is an acid-expressed gene. This regulation is probably mediated by Rim101p through the consensus site 5′-GCCARG-3′ at 237 bp preceding the UGA4 coding sequence (201). © 2001 Elsevier Science Ltd. All rights reserved. Fil:Batlle, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2001 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13572725_v33_n12_p1202_Moretti http://hdl.handle.net/20.500.12110/paper_13572725_v33_n12_p1202_Moretti |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
γ-aminobutyric acid δ-aminolevulinic acid Ambient pH Saccharomyces cerevisiae Transcriptional regulation UGA4 gene 4 aminobutyric acid acid aminolevulinic acid bacterial enzyme beta galactosidase permease protein derivative protein Rim101p transcription factor unclassified drug acidity article binding site consensus sequence controlled study enzyme activity fusion gene gene expression genetic code nonhuman pH protein induction repressor gene Saccharomyces cerevisiae transcription regulation Aminolevulinic Acid beta-Galactosidase Binding Sites GABA Plasma Membrane Transport Proteins Hydrogen-Ion Concentration Lac Operon Organic Anion Transporters Promoter Regions (Genetics) Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Saccharomyces cerevisiae |
| spellingShingle |
γ-aminobutyric acid δ-aminolevulinic acid Ambient pH Saccharomyces cerevisiae Transcriptional regulation UGA4 gene 4 aminobutyric acid acid aminolevulinic acid bacterial enzyme beta galactosidase permease protein derivative protein Rim101p transcription factor unclassified drug acidity article binding site consensus sequence controlled study enzyme activity fusion gene gene expression genetic code nonhuman pH protein induction repressor gene Saccharomyces cerevisiae transcription regulation Aminolevulinic Acid beta-Galactosidase Binding Sites GABA Plasma Membrane Transport Proteins Hydrogen-Ion Concentration Lac Operon Organic Anion Transporters Promoter Regions (Genetics) Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Saccharomyces cerevisiae Batlle, Alcira María del Carmen UGA4 gene encoding the γ-aminobutyric acid permease in Saccharomyces cerevisiae is an acid-expressed gene |
| topic_facet |
γ-aminobutyric acid δ-aminolevulinic acid Ambient pH Saccharomyces cerevisiae Transcriptional regulation UGA4 gene 4 aminobutyric acid acid aminolevulinic acid bacterial enzyme beta galactosidase permease protein derivative protein Rim101p transcription factor unclassified drug acidity article binding site consensus sequence controlled study enzyme activity fusion gene gene expression genetic code nonhuman pH protein induction repressor gene Saccharomyces cerevisiae transcription regulation Aminolevulinic Acid beta-Galactosidase Binding Sites GABA Plasma Membrane Transport Proteins Hydrogen-Ion Concentration Lac Operon Organic Anion Transporters Promoter Regions (Genetics) Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Saccharomyces cerevisiae |
| description |
Background and aims: Biological processes in all organisms are controlled by environmental conditions, however, information concerning the molecular responses to external pH is scarce. In this work we studied the pH response of UGA4 gene encoding δ-aminolevulinic acid and γ-aminobutyric acid permease in Saccharomyces cerevisiae. Methods: We analyzed the effect of pH on the expression of UGA4 gene measuring β-galactosidase activity in cells carrying a UGA4::lacZ fusion gene. Results: Results indicate that UGA4 expression is higher at acidic pH. The expression of UGA3 and UGA35 genes, which encode two positive transcription factors, is not regulated by external pH, while the expression of UGA43 gene encoding a repressor of UGA4 transcription is dependent on pH. Using a strain lacking Uga43p we clearly showed that the effect of ambient pH on UGA4 expression is not a secondary effect of the pH regulation on UGA43. We have also demonstrated that the effect of pH can only be detected when UGA4 gene is not subject to a strong repression by Uga43p nor to GABA induction. Conclusion: Here, we demonstrate that UGA4 is an acid-expressed gene. This regulation is probably mediated by Rim101p through the consensus site 5′-GCCARG-3′ at 237 bp preceding the UGA4 coding sequence (201). © 2001 Elsevier Science Ltd. All rights reserved. |
| author |
Batlle, Alcira María del Carmen |
| author_facet |
Batlle, Alcira María del Carmen |
| author_sort |
Batlle, Alcira María del Carmen |
| title |
UGA4 gene encoding the γ-aminobutyric acid permease in Saccharomyces cerevisiae is an acid-expressed gene |
| title_short |
UGA4 gene encoding the γ-aminobutyric acid permease in Saccharomyces cerevisiae is an acid-expressed gene |
| title_full |
UGA4 gene encoding the γ-aminobutyric acid permease in Saccharomyces cerevisiae is an acid-expressed gene |
| title_fullStr |
UGA4 gene encoding the γ-aminobutyric acid permease in Saccharomyces cerevisiae is an acid-expressed gene |
| title_full_unstemmed |
UGA4 gene encoding the γ-aminobutyric acid permease in Saccharomyces cerevisiae is an acid-expressed gene |
| title_sort |
uga4 gene encoding the γ-aminobutyric acid permease in saccharomyces cerevisiae is an acid-expressed gene |
| publishDate |
2001 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13572725_v33_n12_p1202_Moretti http://hdl.handle.net/20.500.12110/paper_13572725_v33_n12_p1202_Moretti |
| work_keys_str_mv |
AT batllealciramariadelcarmen uga4geneencodingthegaminobutyricacidpermeaseinsaccharomycescerevisiaeisanacidexpressedgene |
| _version_ |
1768544379104919552 |