Regulation of ovarian follicle differentiation in gonadotrophin-stimulated rats
The aim of the present study was to investigate the regulation of the in vitro DNA synthesis of ovarian cells recovered from prepubertal rats 48 h after administration of pregnant mare's serum gonadotrophin alone (granulosa cells) or followed by human chorionic gonadotrophin (luteal cells). Iso...
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1997
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Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1355008X_v6_n2_p145_Andreu http://hdl.handle.net/20.500.12110/paper_1355008X_v6_n2_p145_Andreu |
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paper:paper_1355008X_v6_n2_p145_Andreu2023-06-08T16:11:06Z Regulation of ovarian follicle differentiation in gonadotrophin-stimulated rats Andreu, Claudia Viviana Buscaglia, Carlos Andrés Parborell, M.Fernanda A. Stein, Paula Tesone, Marta Corpus luteum Follicle differentiation Granulosa cell Ovary chorionic gonadotropin estradiol follitropin gonadotropin luteinizing hormone novormon seric gonadotropin thymidine unclassified drug animal cell animal experiment article controlled study corpus luteum female granulosa cell luteal cell nonhuman ovary follicle priority journal rat Animals Cell Differentiation Cells, Cultured Corpus Luteum Culture Media, Conditioned DNA Female Gonadotropins Gonadotropins, Equine Granulosa Cells Ovarian Follicle Ovary Pregnancy Rats Rats, Sprague-Dawley Steroids Stimulation, Chemical Thymidine The aim of the present study was to investigate the regulation of the in vitro DNA synthesis of ovarian cells recovered from prepubertal rats 48 h after administration of pregnant mare's serum gonadotrophin alone (granulosa cells) or followed by human chorionic gonadotrophin (luteal cells). Isolated granulosa cells were cultured in serum-free medium, different stimuli added for periods of 48 h, and 3H-thymidine incorporation was measured. Both follicle-stimulating hormone (FSH) and luteinizing hormone (LH) inhibited 3H-thymidine incorporation by cultured granulosa cells in a dose-dependent manner (FSH: 10, 100, 200 ng/mL = 26, 41, 49% inhibition, respectively; LH: 0.1, 1, 10 ng/mL = 11, 37, 75% inhibition, respectively). On the other hand, estradiol was found to stimulate 3H-thymidine incorporation in granulosa cells (Estradiol: 5, 50, 500 ng/mL = 17, 37, 76% stimulation, respectively). In luteal cells, the rate of basal 3H-thymidine incorporation was very low (granulosa cells: 2560 ± 310; luteal cells: 661 ± 92 cpm/100,000 cells) and not modified by any stimulus. To determine the possible production of an inhibitory growth factor by the early corpus luteum, 3H-thymidine incorporation by granulosa cells was assessed in the presence of 10% conditioned media (CM) recovered from luteal cell cultures. A marked inhibition both in basal and estradiol-stimulated 3H-thymidine incorporation was observed (74 and 76% of inhibition, respectively). Results suggest that an inhibitory growth factor produced by luteal cells after luteinizing gonadotrophin stimulus could be involved in the differentiation of growing follicles to corpus luteum. Fil:Andreu, C.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Buscaglia, C.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Parborell, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Stein, P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Tesone, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1997 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1355008X_v6_n2_p145_Andreu http://hdl.handle.net/20.500.12110/paper_1355008X_v6_n2_p145_Andreu |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
Corpus luteum Follicle differentiation Granulosa cell Ovary chorionic gonadotropin estradiol follitropin gonadotropin luteinizing hormone novormon seric gonadotropin thymidine unclassified drug animal cell animal experiment article controlled study corpus luteum female granulosa cell luteal cell nonhuman ovary follicle priority journal rat Animals Cell Differentiation Cells, Cultured Corpus Luteum Culture Media, Conditioned DNA Female Gonadotropins Gonadotropins, Equine Granulosa Cells Ovarian Follicle Ovary Pregnancy Rats Rats, Sprague-Dawley Steroids Stimulation, Chemical Thymidine |
spellingShingle |
Corpus luteum Follicle differentiation Granulosa cell Ovary chorionic gonadotropin estradiol follitropin gonadotropin luteinizing hormone novormon seric gonadotropin thymidine unclassified drug animal cell animal experiment article controlled study corpus luteum female granulosa cell luteal cell nonhuman ovary follicle priority journal rat Animals Cell Differentiation Cells, Cultured Corpus Luteum Culture Media, Conditioned DNA Female Gonadotropins Gonadotropins, Equine Granulosa Cells Ovarian Follicle Ovary Pregnancy Rats Rats, Sprague-Dawley Steroids Stimulation, Chemical Thymidine Andreu, Claudia Viviana Buscaglia, Carlos Andrés Parborell, M.Fernanda A. Stein, Paula Tesone, Marta Regulation of ovarian follicle differentiation in gonadotrophin-stimulated rats |
topic_facet |
Corpus luteum Follicle differentiation Granulosa cell Ovary chorionic gonadotropin estradiol follitropin gonadotropin luteinizing hormone novormon seric gonadotropin thymidine unclassified drug animal cell animal experiment article controlled study corpus luteum female granulosa cell luteal cell nonhuman ovary follicle priority journal rat Animals Cell Differentiation Cells, Cultured Corpus Luteum Culture Media, Conditioned DNA Female Gonadotropins Gonadotropins, Equine Granulosa Cells Ovarian Follicle Ovary Pregnancy Rats Rats, Sprague-Dawley Steroids Stimulation, Chemical Thymidine |
description |
The aim of the present study was to investigate the regulation of the in vitro DNA synthesis of ovarian cells recovered from prepubertal rats 48 h after administration of pregnant mare's serum gonadotrophin alone (granulosa cells) or followed by human chorionic gonadotrophin (luteal cells). Isolated granulosa cells were cultured in serum-free medium, different stimuli added for periods of 48 h, and 3H-thymidine incorporation was measured. Both follicle-stimulating hormone (FSH) and luteinizing hormone (LH) inhibited 3H-thymidine incorporation by cultured granulosa cells in a dose-dependent manner (FSH: 10, 100, 200 ng/mL = 26, 41, 49% inhibition, respectively; LH: 0.1, 1, 10 ng/mL = 11, 37, 75% inhibition, respectively). On the other hand, estradiol was found to stimulate 3H-thymidine incorporation in granulosa cells (Estradiol: 5, 50, 500 ng/mL = 17, 37, 76% stimulation, respectively). In luteal cells, the rate of basal 3H-thymidine incorporation was very low (granulosa cells: 2560 ± 310; luteal cells: 661 ± 92 cpm/100,000 cells) and not modified by any stimulus. To determine the possible production of an inhibitory growth factor by the early corpus luteum, 3H-thymidine incorporation by granulosa cells was assessed in the presence of 10% conditioned media (CM) recovered from luteal cell cultures. A marked inhibition both in basal and estradiol-stimulated 3H-thymidine incorporation was observed (74 and 76% of inhibition, respectively). Results suggest that an inhibitory growth factor produced by luteal cells after luteinizing gonadotrophin stimulus could be involved in the differentiation of growing follicles to corpus luteum. |
author |
Andreu, Claudia Viviana Buscaglia, Carlos Andrés Parborell, M.Fernanda A. Stein, Paula Tesone, Marta |
author_facet |
Andreu, Claudia Viviana Buscaglia, Carlos Andrés Parborell, M.Fernanda A. Stein, Paula Tesone, Marta |
author_sort |
Andreu, Claudia Viviana |
title |
Regulation of ovarian follicle differentiation in gonadotrophin-stimulated rats |
title_short |
Regulation of ovarian follicle differentiation in gonadotrophin-stimulated rats |
title_full |
Regulation of ovarian follicle differentiation in gonadotrophin-stimulated rats |
title_fullStr |
Regulation of ovarian follicle differentiation in gonadotrophin-stimulated rats |
title_full_unstemmed |
Regulation of ovarian follicle differentiation in gonadotrophin-stimulated rats |
title_sort |
regulation of ovarian follicle differentiation in gonadotrophin-stimulated rats |
publishDate |
1997 |
url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1355008X_v6_n2_p145_Andreu http://hdl.handle.net/20.500.12110/paper_1355008X_v6_n2_p145_Andreu |
work_keys_str_mv |
AT andreuclaudiaviviana regulationofovarianfollicledifferentiationingonadotrophinstimulatedrats AT buscagliacarlosandres regulationofovarianfollicledifferentiationingonadotrophinstimulatedrats AT parborellmfernandaa regulationofovarianfollicledifferentiationingonadotrophinstimulatedrats AT steinpaula regulationofovarianfollicledifferentiationingonadotrophinstimulatedrats AT tesonemarta regulationofovarianfollicledifferentiationingonadotrophinstimulatedrats |
_version_ |
1768546034649137152 |