Thyroid hormone effect in human hepatocytes

We have already demonstrated that a combined treatment of methimazole and an antioxidant mixture improved the condition of hyperthyroid patients both biochemically and clinically. Elevated thyroid hormone levels might trigger signs and symptoms of hyperthyroidism through the increase of free radical...

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Autores principales:	Miler, Eliana Andrea, Ríos de Molina, María del Carmen, Guerra, Liliana Noemí
Publicado:	2008
Materias:	Human hepatocytes Oxidative stress Thyroxine ascorbic acid catalase free radical glutathione disulfide glutathione peroxidase liothyronine malonaldehyde superoxide dismutase antioxidant activity apoptosis article cell culture cell level cell strain HepG2 controlled study cytotoxicity enzyme activity human human cell in vitro study liver cell oxidative stress priority journal Antioxidants Ascorbic Acid Catalase Cell Line, Tumor Dose-Response Relationship, Drug Glutathione Disulfide Glutathione Peroxidase Hepatocytes Humans Malondialdehyde Superoxide Dismutase Triiodothyronine
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13510002_v13_n4_p185_Miler http://hdl.handle.net/20.500.12110/paper_13510002_v13_n4_p185_Miler
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_13510002_v13_n4_p185_Miler
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spelling	paper:paper_13510002_v13_n4_p185_Miler2023-06-08T16:10:57Z Thyroid hormone effect in human hepatocytes Miler, Eliana Andrea Ríos de Molina, María del Carmen Guerra, Liliana Noemí Human hepatocytes Oxidative stress Thyroxine ascorbic acid catalase free radical glutathione disulfide glutathione peroxidase liothyronine malonaldehyde superoxide dismutase antioxidant activity apoptosis article cell culture cell level cell strain HepG2 controlled study cytotoxicity enzyme activity human human cell in vitro study liver cell oxidative stress priority journal Antioxidants Ascorbic Acid Catalase Cell Line, Tumor Dose-Response Relationship, Drug Glutathione Disulfide Glutathione Peroxidase Hepatocytes Humans Malondialdehyde Superoxide Dismutase Triiodothyronine We have already demonstrated that a combined treatment of methimazole and an antioxidant mixture improved the condition of hyperthyroid patients both biochemically and clinically. Elevated thyroid hormone levels might trigger signs and symptoms of hyperthyroidism through the increase of free radicals. To study the direct effect of thyroid hormone on cellular markers of oxidative stress, we carried out in vitro assays in which 0.1-20.0 nM T3 (6.5-1300.0 ng/dl) doses were added to culture media of the human hepatocyte cell line Hep G2 for 1-24 h. T3 increased malondialdehyde (MDA) and intracellular oxidized glutathione (GSSG) levels; SOD activity was also higher with hormone treatment, whereas catalase and glutathione peroxidase activities showed no variation at different T3 doses and during all experimental times. When ascorbic acid was added to the culture, the MDA level decreased and SOD activity was increased. With higher doses of T3 (e.g. 200 nM), cell death occurred (69% of apoptotic cells). The increase in SOD activity was not enough to overcome the effect of T3 since MDA and GSSG remained high during a 24-h experiment. We showed a beneficial effect of ascorbic acid when cells were exposed to a T3 dose of 20 nM, a higher level of hormone than that achieved in hyperthyroidism. © 2008 W. S. Maney and Son Ltd. Fil:Miler, E.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ríos de Molina, M. del C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Guerra, L.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2008 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13510002_v13_n4_p185_Miler http://hdl.handle.net/20.500.12110/paper_13510002_v13_n4_p185_Miler
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Human hepatocytes Oxidative stress Thyroxine ascorbic acid catalase free radical glutathione disulfide glutathione peroxidase liothyronine malonaldehyde superoxide dismutase antioxidant activity apoptosis article cell culture cell level cell strain HepG2 controlled study cytotoxicity enzyme activity human human cell in vitro study liver cell oxidative stress priority journal Antioxidants Ascorbic Acid Catalase Cell Line, Tumor Dose-Response Relationship, Drug Glutathione Disulfide Glutathione Peroxidase Hepatocytes Humans Malondialdehyde Superoxide Dismutase Triiodothyronine
spellingShingle	Human hepatocytes Oxidative stress Thyroxine ascorbic acid catalase free radical glutathione disulfide glutathione peroxidase liothyronine malonaldehyde superoxide dismutase antioxidant activity apoptosis article cell culture cell level cell strain HepG2 controlled study cytotoxicity enzyme activity human human cell in vitro study liver cell oxidative stress priority journal Antioxidants Ascorbic Acid Catalase Cell Line, Tumor Dose-Response Relationship, Drug Glutathione Disulfide Glutathione Peroxidase Hepatocytes Humans Malondialdehyde Superoxide Dismutase Triiodothyronine Miler, Eliana Andrea Ríos de Molina, María del Carmen Guerra, Liliana Noemí Thyroid hormone effect in human hepatocytes
topic_facet	Human hepatocytes Oxidative stress Thyroxine ascorbic acid catalase free radical glutathione disulfide glutathione peroxidase liothyronine malonaldehyde superoxide dismutase antioxidant activity apoptosis article cell culture cell level cell strain HepG2 controlled study cytotoxicity enzyme activity human human cell in vitro study liver cell oxidative stress priority journal Antioxidants Ascorbic Acid Catalase Cell Line, Tumor Dose-Response Relationship, Drug Glutathione Disulfide Glutathione Peroxidase Hepatocytes Humans Malondialdehyde Superoxide Dismutase Triiodothyronine
description	We have already demonstrated that a combined treatment of methimazole and an antioxidant mixture improved the condition of hyperthyroid patients both biochemically and clinically. Elevated thyroid hormone levels might trigger signs and symptoms of hyperthyroidism through the increase of free radicals. To study the direct effect of thyroid hormone on cellular markers of oxidative stress, we carried out in vitro assays in which 0.1-20.0 nM T3 (6.5-1300.0 ng/dl) doses were added to culture media of the human hepatocyte cell line Hep G2 for 1-24 h. T3 increased malondialdehyde (MDA) and intracellular oxidized glutathione (GSSG) levels; SOD activity was also higher with hormone treatment, whereas catalase and glutathione peroxidase activities showed no variation at different T3 doses and during all experimental times. When ascorbic acid was added to the culture, the MDA level decreased and SOD activity was increased. With higher doses of T3 (e.g. 200 nM), cell death occurred (69% of apoptotic cells). The increase in SOD activity was not enough to overcome the effect of T3 since MDA and GSSG remained high during a 24-h experiment. We showed a beneficial effect of ascorbic acid when cells were exposed to a T3 dose of 20 nM, a higher level of hormone than that achieved in hyperthyroidism. © 2008 W. S. Maney and Son Ltd.
author	Miler, Eliana Andrea Ríos de Molina, María del Carmen Guerra, Liliana Noemí
author_facet	Miler, Eliana Andrea Ríos de Molina, María del Carmen Guerra, Liliana Noemí
author_sort	Miler, Eliana Andrea
title	Thyroid hormone effect in human hepatocytes
title_short	Thyroid hormone effect in human hepatocytes
title_full	Thyroid hormone effect in human hepatocytes
title_fullStr	Thyroid hormone effect in human hepatocytes
title_full_unstemmed	Thyroid hormone effect in human hepatocytes
title_sort	thyroid hormone effect in human hepatocytes
publishDate	2008
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13510002_v13_n4_p185_Miler http://hdl.handle.net/20.500.12110/paper_13510002_v13_n4_p185_Miler
work_keys_str_mv	AT milerelianaandrea thyroidhormoneeffectinhumanhepatocytes AT riosdemolinamariadelcarmen thyroidhormoneeffectinhumanhepatocytes AT guerraliliananoemi thyroidhormoneeffectinhumanhepatocytes
_version_	1768543717884428288

Thyroid hormone effect in human hepatocytes

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