Interplay between the transcription factors acting on the GATA- and GABA-responsive elements of Saccharomyces cerevisiae UGA promoters
γ-Aminobutyric acid (GABA) transport and catabolism in Saccharomyces cerevisiae are subject to a complex transcriptional control that depends on the nutritional status of the cells. The expression of the genes that form the UGA regulon is inducible by GABA and sensitive to nitrogen catabolite repres...
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2012
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13500872_v158_n4_p925_Cardillo http://hdl.handle.net/20.500.12110/paper_13500872_v158_n4_p925_Cardillo |
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paper:paper_13500872_v158_n4_p925_Cardillo2023-06-08T16:10:47Z Interplay between the transcription factors acting on the GATA- and GABA-responsive elements of Saccharomyces cerevisiae UGA promoters Cardillo, Sabrina Beatriz 4 aminobutyric acid polyglutamine transcription factor transcription factor Dal80 transcription factor Dal81 transcription factor GATA transcription factor Gln3 transcription factor Uga3 unclassified drug zinc article catabolite repression controlled study fungal gene gene expression gene interaction gene mutation nitrogen catabolite repression nonhuman priority journal promoter region protein DNA binding protein domain regulon reverse transcription polymerase chain reaction Saccharomyces cerevisiae transcription initiation transcription regulation transcription termination DNA-Binding Proteins gamma-Aminobutyric Acid GATA Transcription Factors Gene Expression Regulation, Fungal Promoter Regions, Genetic Regulon Repressor Proteins Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Transcription Factors Saccharomyces cerevisiae γ-Aminobutyric acid (GABA) transport and catabolism in Saccharomyces cerevisiae are subject to a complex transcriptional control that depends on the nutritional status of the cells. The expression of the genes that form the UGA regulon is inducible by GABA and sensitive to nitrogen catabolite repression (NCR). GABA induction of these genes is mediated by Uga3 and Dal81 transcription factors, whereas GATA factors are responsible for NCR. Here, we show how members of the UGA regulon share the activation mechanism. Our results show that both Uga3 and Dal81 interact with UGA genes in a GABA-dependent manner, and that they depend on each other for the interaction with their target promoters and the transcriptional activation. The typical DNA-binding domain Zn(II) 2-Cys 6 of Dal81 is unnecessary for its activity and Uga3 acts as a bridge between Dal81 and DNA. Both the trans-activation activity of the GATA factor Gln3 and the repressive activity of the GATA factor Dal80 are exerted by their interaction with UGA promoters in response to GABA, indicating that Uga3, Dal81, Gln3 and Dal80 all act in concert to induce the expression of UGA genes. So, an interplay between the factors responsible for GABA induction and those responsible for NCR in the regulation of the UGA genes is proposed here. © 2012 SGM. Fil:Cardillo, S.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2012 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13500872_v158_n4_p925_Cardillo http://hdl.handle.net/20.500.12110/paper_13500872_v158_n4_p925_Cardillo |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
4 aminobutyric acid polyglutamine transcription factor transcription factor Dal80 transcription factor Dal81 transcription factor GATA transcription factor Gln3 transcription factor Uga3 unclassified drug zinc article catabolite repression controlled study fungal gene gene expression gene interaction gene mutation nitrogen catabolite repression nonhuman priority journal promoter region protein DNA binding protein domain regulon reverse transcription polymerase chain reaction Saccharomyces cerevisiae transcription initiation transcription regulation transcription termination DNA-Binding Proteins gamma-Aminobutyric Acid GATA Transcription Factors Gene Expression Regulation, Fungal Promoter Regions, Genetic Regulon Repressor Proteins Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Transcription Factors Saccharomyces cerevisiae |
| spellingShingle |
4 aminobutyric acid polyglutamine transcription factor transcription factor Dal80 transcription factor Dal81 transcription factor GATA transcription factor Gln3 transcription factor Uga3 unclassified drug zinc article catabolite repression controlled study fungal gene gene expression gene interaction gene mutation nitrogen catabolite repression nonhuman priority journal promoter region protein DNA binding protein domain regulon reverse transcription polymerase chain reaction Saccharomyces cerevisiae transcription initiation transcription regulation transcription termination DNA-Binding Proteins gamma-Aminobutyric Acid GATA Transcription Factors Gene Expression Regulation, Fungal Promoter Regions, Genetic Regulon Repressor Proteins Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Transcription Factors Saccharomyces cerevisiae Cardillo, Sabrina Beatriz Interplay between the transcription factors acting on the GATA- and GABA-responsive elements of Saccharomyces cerevisiae UGA promoters |
| topic_facet |
4 aminobutyric acid polyglutamine transcription factor transcription factor Dal80 transcription factor Dal81 transcription factor GATA transcription factor Gln3 transcription factor Uga3 unclassified drug zinc article catabolite repression controlled study fungal gene gene expression gene interaction gene mutation nitrogen catabolite repression nonhuman priority journal promoter region protein DNA binding protein domain regulon reverse transcription polymerase chain reaction Saccharomyces cerevisiae transcription initiation transcription regulation transcription termination DNA-Binding Proteins gamma-Aminobutyric Acid GATA Transcription Factors Gene Expression Regulation, Fungal Promoter Regions, Genetic Regulon Repressor Proteins Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Transcription Factors Saccharomyces cerevisiae |
| description |
γ-Aminobutyric acid (GABA) transport and catabolism in Saccharomyces cerevisiae are subject to a complex transcriptional control that depends on the nutritional status of the cells. The expression of the genes that form the UGA regulon is inducible by GABA and sensitive to nitrogen catabolite repression (NCR). GABA induction of these genes is mediated by Uga3 and Dal81 transcription factors, whereas GATA factors are responsible for NCR. Here, we show how members of the UGA regulon share the activation mechanism. Our results show that both Uga3 and Dal81 interact with UGA genes in a GABA-dependent manner, and that they depend on each other for the interaction with their target promoters and the transcriptional activation. The typical DNA-binding domain Zn(II) 2-Cys 6 of Dal81 is unnecessary for its activity and Uga3 acts as a bridge between Dal81 and DNA. Both the trans-activation activity of the GATA factor Gln3 and the repressive activity of the GATA factor Dal80 are exerted by their interaction with UGA promoters in response to GABA, indicating that Uga3, Dal81, Gln3 and Dal80 all act in concert to induce the expression of UGA genes. So, an interplay between the factors responsible for GABA induction and those responsible for NCR in the regulation of the UGA genes is proposed here. © 2012 SGM. |
| author |
Cardillo, Sabrina Beatriz |
| author_facet |
Cardillo, Sabrina Beatriz |
| author_sort |
Cardillo, Sabrina Beatriz |
| title |
Interplay between the transcription factors acting on the GATA- and GABA-responsive elements of Saccharomyces cerevisiae UGA promoters |
| title_short |
Interplay between the transcription factors acting on the GATA- and GABA-responsive elements of Saccharomyces cerevisiae UGA promoters |
| title_full |
Interplay between the transcription factors acting on the GATA- and GABA-responsive elements of Saccharomyces cerevisiae UGA promoters |
| title_fullStr |
Interplay between the transcription factors acting on the GATA- and GABA-responsive elements of Saccharomyces cerevisiae UGA promoters |
| title_full_unstemmed |
Interplay between the transcription factors acting on the GATA- and GABA-responsive elements of Saccharomyces cerevisiae UGA promoters |
| title_sort |
interplay between the transcription factors acting on the gata- and gaba-responsive elements of saccharomyces cerevisiae uga promoters |
| publishDate |
2012 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13500872_v158_n4_p925_Cardillo http://hdl.handle.net/20.500.12110/paper_13500872_v158_n4_p925_Cardillo |
| work_keys_str_mv |
AT cardillosabrinabeatriz interplaybetweenthetranscriptionfactorsactingonthegataandgabaresponsiveelementsofsaccharomycescerevisiaeugapromoters |
| _version_ |
1768545845418917888 |