New insights into the regulation of the Saccharomyces cerevisiae UGA54 gene: Two parallel pathways participate in carbon-regulated transcription
The Saccharomyces cerevisiae UGA4 gene, which encodes the γ-aminobutyric acid (GABA) and δ-aminolaevulinic acid (ALA) permease, is well known to be regulated by the nitrogen source. Its expression levels are low in the presence of a rich nitrogen source but are higher when a poor nitrogen source is...
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2007
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13500872_v153_n11_p3677_Luzzani http://hdl.handle.net/20.500.12110/paper_13500872_v153_n11_p3677_Luzzani |
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paper:paper_13500872_v153_n11_p3677_Luzzani2023-06-08T16:10:46Z New insights into the regulation of the Saccharomyces cerevisiae UGA54 gene: Two parallel pathways participate in carbon-regulated transcription Luzzani, Carlos Daniel Cardillo, Sabrina Beatriz 4 aminobutyric acid acetic acid aminolevulinic acid carbon glucose nitrogen permease repressor protein transcription factor transcription factor GAT1 transcription factor GATA transcription factor gln3 transcription factor GZF3 UGA4 protein Uga43 protein unclassified drug article carbon source cellular distribution controlled study gene control gene deletion gene expression regulation nonhuman priority journal promoter region protein localization Saccharomyces cerevisiae site directed mutagenesis transcription regulation Acetates Carbon Culture Media GABA Plasma Membrane Transport Proteins GATA Transcription Factors Gene Expression Regulation, Fungal Glucose Mutation Nitrogen Promoter Regions (Genetics) Repressor Proteins Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Transcription Factors Transcription, Genetic Saccharomyces cerevisiae The Saccharomyces cerevisiae UGA4 gene, which encodes the γ-aminobutyric acid (GABA) and δ-aminolaevulinic acid (ALA) permease, is well known to be regulated by the nitrogen source. Its expression levels are low in the presence of a rich nitrogen source but are higher when a poor nitrogen source is used. In addition, GABA can induce UGA4 expression when cells are grown with proline but not when they are grown with ammonium. Although vast amounts of evidence have been gathered about UGA4 regulation by nitrogen, little is known about its regulation by the carbon source. Using glucose and acetate as rich and poor carbon source respectively, this work aimed to shed light on hitherto unclear aspects of the regulation of this gene. In poor nitrogen conditions, cells grown with acetate were found to have higher UGA4 basal expression levels than those grown with glucose, and did not show UGA4 induction in response to GABA. Analysis of the expression and subcellular localization of the transcription factors that regulate UGA4 as well as partial deletions and site-directed mutations of the UGA4 promoter region suggested that there are two parallel pathways that act in regulating this gene by the carbon source. Furthermore, the results demonstrate the existence of a new factor operating in UGA4 regulation. © 2007 SGM. Fil:Luzzani, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cardillo, S.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2007 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13500872_v153_n11_p3677_Luzzani http://hdl.handle.net/20.500.12110/paper_13500872_v153_n11_p3677_Luzzani |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
4 aminobutyric acid acetic acid aminolevulinic acid carbon glucose nitrogen permease repressor protein transcription factor transcription factor GAT1 transcription factor GATA transcription factor gln3 transcription factor GZF3 UGA4 protein Uga43 protein unclassified drug article carbon source cellular distribution controlled study gene control gene deletion gene expression regulation nonhuman priority journal promoter region protein localization Saccharomyces cerevisiae site directed mutagenesis transcription regulation Acetates Carbon Culture Media GABA Plasma Membrane Transport Proteins GATA Transcription Factors Gene Expression Regulation, Fungal Glucose Mutation Nitrogen Promoter Regions (Genetics) Repressor Proteins Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Transcription Factors Transcription, Genetic Saccharomyces cerevisiae |
| spellingShingle |
4 aminobutyric acid acetic acid aminolevulinic acid carbon glucose nitrogen permease repressor protein transcription factor transcription factor GAT1 transcription factor GATA transcription factor gln3 transcription factor GZF3 UGA4 protein Uga43 protein unclassified drug article carbon source cellular distribution controlled study gene control gene deletion gene expression regulation nonhuman priority journal promoter region protein localization Saccharomyces cerevisiae site directed mutagenesis transcription regulation Acetates Carbon Culture Media GABA Plasma Membrane Transport Proteins GATA Transcription Factors Gene Expression Regulation, Fungal Glucose Mutation Nitrogen Promoter Regions (Genetics) Repressor Proteins Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Transcription Factors Transcription, Genetic Saccharomyces cerevisiae Luzzani, Carlos Daniel Cardillo, Sabrina Beatriz New insights into the regulation of the Saccharomyces cerevisiae UGA54 gene: Two parallel pathways participate in carbon-regulated transcription |
| topic_facet |
4 aminobutyric acid acetic acid aminolevulinic acid carbon glucose nitrogen permease repressor protein transcription factor transcription factor GAT1 transcription factor GATA transcription factor gln3 transcription factor GZF3 UGA4 protein Uga43 protein unclassified drug article carbon source cellular distribution controlled study gene control gene deletion gene expression regulation nonhuman priority journal promoter region protein localization Saccharomyces cerevisiae site directed mutagenesis transcription regulation Acetates Carbon Culture Media GABA Plasma Membrane Transport Proteins GATA Transcription Factors Gene Expression Regulation, Fungal Glucose Mutation Nitrogen Promoter Regions (Genetics) Repressor Proteins Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Transcription Factors Transcription, Genetic Saccharomyces cerevisiae |
| description |
The Saccharomyces cerevisiae UGA4 gene, which encodes the γ-aminobutyric acid (GABA) and δ-aminolaevulinic acid (ALA) permease, is well known to be regulated by the nitrogen source. Its expression levels are low in the presence of a rich nitrogen source but are higher when a poor nitrogen source is used. In addition, GABA can induce UGA4 expression when cells are grown with proline but not when they are grown with ammonium. Although vast amounts of evidence have been gathered about UGA4 regulation by nitrogen, little is known about its regulation by the carbon source. Using glucose and acetate as rich and poor carbon source respectively, this work aimed to shed light on hitherto unclear aspects of the regulation of this gene. In poor nitrogen conditions, cells grown with acetate were found to have higher UGA4 basal expression levels than those grown with glucose, and did not show UGA4 induction in response to GABA. Analysis of the expression and subcellular localization of the transcription factors that regulate UGA4 as well as partial deletions and site-directed mutations of the UGA4 promoter region suggested that there are two parallel pathways that act in regulating this gene by the carbon source. Furthermore, the results demonstrate the existence of a new factor operating in UGA4 regulation. © 2007 SGM. |
| author |
Luzzani, Carlos Daniel Cardillo, Sabrina Beatriz |
| author_facet |
Luzzani, Carlos Daniel Cardillo, Sabrina Beatriz |
| author_sort |
Luzzani, Carlos Daniel |
| title |
New insights into the regulation of the Saccharomyces cerevisiae UGA54 gene: Two parallel pathways participate in carbon-regulated transcription |
| title_short |
New insights into the regulation of the Saccharomyces cerevisiae UGA54 gene: Two parallel pathways participate in carbon-regulated transcription |
| title_full |
New insights into the regulation of the Saccharomyces cerevisiae UGA54 gene: Two parallel pathways participate in carbon-regulated transcription |
| title_fullStr |
New insights into the regulation of the Saccharomyces cerevisiae UGA54 gene: Two parallel pathways participate in carbon-regulated transcription |
| title_full_unstemmed |
New insights into the regulation of the Saccharomyces cerevisiae UGA54 gene: Two parallel pathways participate in carbon-regulated transcription |
| title_sort |
new insights into the regulation of the saccharomyces cerevisiae uga54 gene: two parallel pathways participate in carbon-regulated transcription |
| publishDate |
2007 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13500872_v153_n11_p3677_Luzzani http://hdl.handle.net/20.500.12110/paper_13500872_v153_n11_p3677_Luzzani |
| work_keys_str_mv |
AT luzzanicarlosdaniel newinsightsintotheregulationofthesaccharomycescerevisiaeuga54genetwoparallelpathwaysparticipateincarbonregulatedtranscription AT cardillosabrinabeatriz newinsightsintotheregulationofthesaccharomycescerevisiaeuga54genetwoparallelpathwaysparticipateincarbonregulatedtranscription |
| _version_ |
1768544833010401280 |