Osmoresistance of spores from Bacillus subtilis and the effect of ssp mutations

Spores of Bacillus subtilis show similar plating efficiency on media with or without 1.5 M NaCl. In contrast, vegetative cells are osmosensitive unless the stationary phase has been reached. In the present work, loss of heat and osmotic resistance during germination was studied. Their kinetics and s...

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Detalles Bibliográficos
Autores principales:	Ruzal, Sandra Mónica, Alice, Alejandro Fabián
Publicado:	1994
Materias:	Bacillus subtilis Small acid-soluble protein Spore osmoresistance ssp amino acid antibiotic agent bacterial protein chloramphenicol glutamic acid sodium chloride article assay bacterial spore gene mutation genetic complementation germination heat loss heat tolerance kinetics nonhuman osmosis priority journal protein degradation protein synthesis inhibition Bacteria (microorganisms)
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13500872_v140_n8_p2173_Ruzal http://hdl.handle.net/20.500.12110/paper_13500872_v140_n8_p2173_Ruzal
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_13500872_v140_n8_p2173_Ruzal
record_format	dspace
spelling	paper:paper_13500872_v140_n8_p2173_Ruzal2023-06-08T16:10:44Z Osmoresistance of spores from Bacillus subtilis and the effect of ssp mutations Ruzal, Sandra Mónica Alice, Alejandro Fabián Bacillus subtilis Small acid-soluble protein Spore osmoresistance ssp amino acid antibiotic agent bacterial protein chloramphenicol glutamic acid sodium chloride article assay Bacillus subtilis bacterial spore gene mutation genetic complementation germination heat loss heat tolerance kinetics nonhuman osmosis priority journal protein degradation protein synthesis inhibition Bacillus subtilis Bacteria (microorganisms) Spores of Bacillus subtilis show similar plating efficiency on media with or without 1.5 M NaCl. In contrast, vegetative cells are osmosensitive unless the stationary phase has been reached. In the present work, loss of heat and osmotic resistance during germination was studied. Their kinetics and sensitivity to protein synthesis inhibition were different: heat resistance was lost first and even in the presence of chloramphenicol, whereas loss of osmotolerance occurred later and was inhibited in the presence of this antibiotic. The influence of spore-associated small acid-soluble proteins (SASPs) on spore osmotolerance was investigated using ssp mutants: all produced spores which germinated poorly and were sensitive to osmotic strength. SASP-E deficiency was particularly significant. Spore osmotolerance was largely restored in complementation assays performed with cloned ssp genes. It is possible that germination-associated degradation of SASP proteins provides osmotically significant levels of amino acids (especially glutamate). Fil:Ruzal, S.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Alice, A.F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1994 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13500872_v140_n8_p2173_Ruzal http://hdl.handle.net/20.500.12110/paper_13500872_v140_n8_p2173_Ruzal
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Bacillus subtilis Small acid-soluble protein Spore osmoresistance ssp amino acid antibiotic agent bacterial protein chloramphenicol glutamic acid sodium chloride article assay Bacillus subtilis bacterial spore gene mutation genetic complementation germination heat loss heat tolerance kinetics nonhuman osmosis priority journal protein degradation protein synthesis inhibition Bacillus subtilis Bacteria (microorganisms)
spellingShingle	Bacillus subtilis Small acid-soluble protein Spore osmoresistance ssp amino acid antibiotic agent bacterial protein chloramphenicol glutamic acid sodium chloride article assay Bacillus subtilis bacterial spore gene mutation genetic complementation germination heat loss heat tolerance kinetics nonhuman osmosis priority journal protein degradation protein synthesis inhibition Bacillus subtilis Bacteria (microorganisms) Ruzal, Sandra Mónica Alice, Alejandro Fabián Osmoresistance of spores from Bacillus subtilis and the effect of ssp mutations
topic_facet	Bacillus subtilis Small acid-soluble protein Spore osmoresistance ssp amino acid antibiotic agent bacterial protein chloramphenicol glutamic acid sodium chloride article assay Bacillus subtilis bacterial spore gene mutation genetic complementation germination heat loss heat tolerance kinetics nonhuman osmosis priority journal protein degradation protein synthesis inhibition Bacillus subtilis Bacteria (microorganisms)
description	Spores of Bacillus subtilis show similar plating efficiency on media with or without 1.5 M NaCl. In contrast, vegetative cells are osmosensitive unless the stationary phase has been reached. In the present work, loss of heat and osmotic resistance during germination was studied. Their kinetics and sensitivity to protein synthesis inhibition were different: heat resistance was lost first and even in the presence of chloramphenicol, whereas loss of osmotolerance occurred later and was inhibited in the presence of this antibiotic. The influence of spore-associated small acid-soluble proteins (SASPs) on spore osmotolerance was investigated using ssp mutants: all produced spores which germinated poorly and were sensitive to osmotic strength. SASP-E deficiency was particularly significant. Spore osmotolerance was largely restored in complementation assays performed with cloned ssp genes. It is possible that germination-associated degradation of SASP proteins provides osmotically significant levels of amino acids (especially glutamate).
author	Ruzal, Sandra Mónica Alice, Alejandro Fabián
author_facet	Ruzal, Sandra Mónica Alice, Alejandro Fabián
author_sort	Ruzal, Sandra Mónica
title	Osmoresistance of spores from Bacillus subtilis and the effect of ssp mutations
title_short	Osmoresistance of spores from Bacillus subtilis and the effect of ssp mutations
title_full	Osmoresistance of spores from Bacillus subtilis and the effect of ssp mutations
title_fullStr	Osmoresistance of spores from Bacillus subtilis and the effect of ssp mutations
title_full_unstemmed	Osmoresistance of spores from Bacillus subtilis and the effect of ssp mutations
title_sort	osmoresistance of spores from bacillus subtilis and the effect of ssp mutations
publishDate	1994
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13500872_v140_n8_p2173_Ruzal http://hdl.handle.net/20.500.12110/paper_13500872_v140_n8_p2173_Ruzal
work_keys_str_mv	AT ruzalsandramonica osmoresistanceofsporesfrombacillussubtilisandtheeffectofsspmutations AT alicealejandrofabian osmoresistanceofsporesfrombacillussubtilisandtheeffectofsspmutations
_version_	1768546456963121152

Osmoresistance of spores from Bacillus subtilis and the effect of ssp mutations

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