Evidence of an association between poly(3-hydroxybutyrate) accumulation and phosphotransbutyrylase expression in Bacillus megaterium
Molecular analysis of a genomic region of Bacillus megaterium, a polyhydroxybutyrate (PHB)-producing microorganism, revealed the presence of a gene coding for the enzyme phosphotransbutyrylase (Ptb). Enzyme activity was measured throughout the different growth phases of B. megaterium and was found t...
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2003
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Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_11396709_v6_n2_p127_Vazquez http://hdl.handle.net/20.500.12110/paper_11396709_v6_n2_p127_Vazquez |
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paper:paper_11396709_v6_n2_p127_Vazquez2023-06-08T16:09:15Z Evidence of an association between poly(3-hydroxybutyrate) accumulation and phosphotransbutyrylase expression in Bacillus megaterium Vazquez, Gustavo Pettinari, María Julia Méndez, Beatriz Silvia Bacillus megaterium Polyhydroxybutyrate Phosphotransbutyrylase enzyme glucose isoleucine phosphotransbutyrylase poly(3 hydroxybutyric acid) unclassified drug valine article Bacillus megaterium bacterial growth controlled study correlation analysis enzyme activity gene location genetic code growth curve nonhuman protein expression Bacillus megaterium Bacteria (microorganisms) Molecular analysis of a genomic region of Bacillus megaterium, a polyhydroxybutyrate (PHB)-producing microorganism, revealed the presence of a gene coding for the enzyme phosphotransbutyrylase (Ptb). Enzyme activity was measured throughout the different growth phases of B. megaterium and was found to correlate with PHB accumulation during the late-exponential growth phase. Ptb expression was repressed by glucose and activated by the branched amino acids isoleucine and valine. Overexpression of ActBm, a σ 54 regulator from B. megaterium whose gene is located upstream from ptb, caused an increase in Ptb activity and PHB accumulation in B. megaterium. Fil:Vazquez, G.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pettinari, M.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Méndez, B.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2003 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_11396709_v6_n2_p127_Vazquez http://hdl.handle.net/20.500.12110/paper_11396709_v6_n2_p127_Vazquez |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
Bacillus megaterium Polyhydroxybutyrate Phosphotransbutyrylase enzyme glucose isoleucine phosphotransbutyrylase poly(3 hydroxybutyric acid) unclassified drug valine article Bacillus megaterium bacterial growth controlled study correlation analysis enzyme activity gene location genetic code growth curve nonhuman protein expression Bacillus megaterium Bacteria (microorganisms) |
spellingShingle |
Bacillus megaterium Polyhydroxybutyrate Phosphotransbutyrylase enzyme glucose isoleucine phosphotransbutyrylase poly(3 hydroxybutyric acid) unclassified drug valine article Bacillus megaterium bacterial growth controlled study correlation analysis enzyme activity gene location genetic code growth curve nonhuman protein expression Bacillus megaterium Bacteria (microorganisms) Vazquez, Gustavo Pettinari, María Julia Méndez, Beatriz Silvia Evidence of an association between poly(3-hydroxybutyrate) accumulation and phosphotransbutyrylase expression in Bacillus megaterium |
topic_facet |
Bacillus megaterium Polyhydroxybutyrate Phosphotransbutyrylase enzyme glucose isoleucine phosphotransbutyrylase poly(3 hydroxybutyric acid) unclassified drug valine article Bacillus megaterium bacterial growth controlled study correlation analysis enzyme activity gene location genetic code growth curve nonhuman protein expression Bacillus megaterium Bacteria (microorganisms) |
description |
Molecular analysis of a genomic region of Bacillus megaterium, a polyhydroxybutyrate (PHB)-producing microorganism, revealed the presence of a gene coding for the enzyme phosphotransbutyrylase (Ptb). Enzyme activity was measured throughout the different growth phases of B. megaterium and was found to correlate with PHB accumulation during the late-exponential growth phase. Ptb expression was repressed by glucose and activated by the branched amino acids isoleucine and valine. Overexpression of ActBm, a σ 54 regulator from B. megaterium whose gene is located upstream from ptb, caused an increase in Ptb activity and PHB accumulation in B. megaterium. |
author |
Vazquez, Gustavo Pettinari, María Julia Méndez, Beatriz Silvia |
author_facet |
Vazquez, Gustavo Pettinari, María Julia Méndez, Beatriz Silvia |
author_sort |
Vazquez, Gustavo |
title |
Evidence of an association between poly(3-hydroxybutyrate) accumulation and phosphotransbutyrylase expression in Bacillus megaterium |
title_short |
Evidence of an association between poly(3-hydroxybutyrate) accumulation and phosphotransbutyrylase expression in Bacillus megaterium |
title_full |
Evidence of an association between poly(3-hydroxybutyrate) accumulation and phosphotransbutyrylase expression in Bacillus megaterium |
title_fullStr |
Evidence of an association between poly(3-hydroxybutyrate) accumulation and phosphotransbutyrylase expression in Bacillus megaterium |
title_full_unstemmed |
Evidence of an association between poly(3-hydroxybutyrate) accumulation and phosphotransbutyrylase expression in Bacillus megaterium |
title_sort |
evidence of an association between poly(3-hydroxybutyrate) accumulation and phosphotransbutyrylase expression in bacillus megaterium |
publishDate |
2003 |
url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_11396709_v6_n2_p127_Vazquez http://hdl.handle.net/20.500.12110/paper_11396709_v6_n2_p127_Vazquez |
work_keys_str_mv |
AT vazquezgustavo evidenceofanassociationbetweenpoly3hydroxybutyrateaccumulationandphosphotransbutyrylaseexpressioninbacillusmegaterium AT pettinarimariajulia evidenceofanassociationbetweenpoly3hydroxybutyrateaccumulationandphosphotransbutyrylaseexpressioninbacillusmegaterium AT mendezbeatrizsilvia evidenceofanassociationbetweenpoly3hydroxybutyrateaccumulationandphosphotransbutyrylaseexpressioninbacillusmegaterium |
_version_ |
1768545891282583552 |