Stereum hirsutum (Wild) Pers. action in dye degradation

Stereum hirsutum, a white rot fungus, has a good growth in solid state fermentation. This was carried on with wheat bran, soy bran and a mixture of both. Mycelia grown on soy bran showed the highest decolonization activity on Ponceau 2R (xylidine), indigo carmine and malachite green. Optimal relatio...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Mouso, Nora, Diorio, Luis Alberto, Forchíassin, Flavia
Publicado:	2007
Materias:	Decolorization Detoxification Laccase Stereum hirsutum White rot fungi indigo carmine laccase lignin malachite green article decolorization electrophoresis enzyme synthesis fungus growth microbial degradation mycelial growth nonhuman nutrient Phanerochaete solid state fermentation soybean wheat bran white rot fungus Fungi Phanerochaete chrysosporium Triticum aestivum
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_11301406_v24_n4_p294_Mouso http://hdl.handle.net/20.500.12110/paper_11301406_v24_n4_p294_Mouso
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_11301406_v24_n4_p294_Mouso
record_format	dspace
spelling	paper:paper_11301406_v24_n4_p294_Mouso2023-06-08T16:09:05Z Stereum hirsutum (Wild) Pers. action in dye degradation Mouso, Nora Diorio, Luis Alberto Forchíassin, Flavia Decolorization Detoxification Laccase Stereum hirsutum White rot fungi indigo carmine laccase lignin malachite green article decolorization electrophoresis enzyme synthesis fungus growth microbial degradation mycelial growth nonhuman nutrient Phanerochaete solid state fermentation soybean Stereum hirsutum wheat bran white rot fungus Fungi Phanerochaete chrysosporium Stereum hirsutum Triticum aestivum Stereum hirsutum, a white rot fungus, has a good growth in solid state fermentation. This was carried on with wheat bran, soy bran and a mixture of both. Mycelia grown on soy bran showed the highest decolonization activity on Ponceau 2R (xylidine), indigo carmine and malachite green. Optimal relationship between decolorization and detoxification of malachite green was 30 g of fresh weight (mycelia plus substrate) in 500 ml malachite green solution, 42 U/l of laccase was measured in this solution. Decolorization was carried on without the addition either of nutrients or mediators. Conditions for maximal decolorization did not agree with those for maximal ligninolytic enzyme production, but effectiveness of laccase activity on decolorization was evidenced by electrophoretic analysis, that allowed laccase identification and its decolorization activity in gels stained with indigo carmine and malachite green, with ABTS as mediator. Detoxification was assayed using the sensible fungus Phanerochaete chrysosporium. ©2007 Revista Iberoamericana de Micología. Fil:Mouso, N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Diorio, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Forchiassin, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2007 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_11301406_v24_n4_p294_Mouso http://hdl.handle.net/20.500.12110/paper_11301406_v24_n4_p294_Mouso
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Decolorization Detoxification Laccase Stereum hirsutum White rot fungi indigo carmine laccase lignin malachite green article decolorization electrophoresis enzyme synthesis fungus growth microbial degradation mycelial growth nonhuman nutrient Phanerochaete solid state fermentation soybean Stereum hirsutum wheat bran white rot fungus Fungi Phanerochaete chrysosporium Stereum hirsutum Triticum aestivum
spellingShingle	Decolorization Detoxification Laccase Stereum hirsutum White rot fungi indigo carmine laccase lignin malachite green article decolorization electrophoresis enzyme synthesis fungus growth microbial degradation mycelial growth nonhuman nutrient Phanerochaete solid state fermentation soybean Stereum hirsutum wheat bran white rot fungus Fungi Phanerochaete chrysosporium Stereum hirsutum Triticum aestivum Mouso, Nora Diorio, Luis Alberto Forchíassin, Flavia Stereum hirsutum (Wild) Pers. action in dye degradation
topic_facet	Decolorization Detoxification Laccase Stereum hirsutum White rot fungi indigo carmine laccase lignin malachite green article decolorization electrophoresis enzyme synthesis fungus growth microbial degradation mycelial growth nonhuman nutrient Phanerochaete solid state fermentation soybean Stereum hirsutum wheat bran white rot fungus Fungi Phanerochaete chrysosporium Stereum hirsutum Triticum aestivum
description	Stereum hirsutum, a white rot fungus, has a good growth in solid state fermentation. This was carried on with wheat bran, soy bran and a mixture of both. Mycelia grown on soy bran showed the highest decolonization activity on Ponceau 2R (xylidine), indigo carmine and malachite green. Optimal relationship between decolorization and detoxification of malachite green was 30 g of fresh weight (mycelia plus substrate) in 500 ml malachite green solution, 42 U/l of laccase was measured in this solution. Decolorization was carried on without the addition either of nutrients or mediators. Conditions for maximal decolorization did not agree with those for maximal ligninolytic enzyme production, but effectiveness of laccase activity on decolorization was evidenced by electrophoretic analysis, that allowed laccase identification and its decolorization activity in gels stained with indigo carmine and malachite green, with ABTS as mediator. Detoxification was assayed using the sensible fungus Phanerochaete chrysosporium. ©2007 Revista Iberoamericana de Micología.
author	Mouso, Nora Diorio, Luis Alberto Forchíassin, Flavia
author_facet	Mouso, Nora Diorio, Luis Alberto Forchíassin, Flavia
author_sort	Mouso, Nora
title	Stereum hirsutum (Wild) Pers. action in dye degradation
title_short	Stereum hirsutum (Wild) Pers. action in dye degradation
title_full	Stereum hirsutum (Wild) Pers. action in dye degradation
title_fullStr	Stereum hirsutum (Wild) Pers. action in dye degradation
title_full_unstemmed	Stereum hirsutum (Wild) Pers. action in dye degradation
title_sort	stereum hirsutum (wild) pers. action in dye degradation
publishDate	2007
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_11301406_v24_n4_p294_Mouso http://hdl.handle.net/20.500.12110/paper_11301406_v24_n4_p294_Mouso
work_keys_str_mv	AT mousonora stereumhirsutumwildpersactionindyedegradation AT diorioluisalberto stereumhirsutumwildpersactionindyedegradation AT forchiassinflavia stereumhirsutumwildpersactionindyedegradation
_version_	1768544790625910784

Stereum hirsutum (Wild) Pers. action in dye degradation

Ejemplares similares