Light Regulates Plant Alternative Splicing through the Control of Transcriptional Elongation
Light makes carbon fixation possible, allowing plant and animal life on Earth. We have previously shown that light regulates alternative splicing in plants. Light initiates a chloroplast retrograde signaling that regulates nuclear alternative splicing of a subset of Arabidopsis thaliana transcripts....
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2019
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10972765_v73_n5_p1066_GodoyHerz http://hdl.handle.net/20.500.12110/paper_10972765_v73_n5_p1066_GodoyHerz |
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paper:paper_10972765_v73_n5_p1066_GodoyHerz2023-06-08T16:07:08Z Light Regulates Plant Alternative Splicing through the Control of Transcriptional Elongation alternative splicing light control in plants transcription elongation histone messenger RNA RNA polymerase II transcription factor II alternative RNA splicing Arabidopsis thaliana Article carbon fixation chloroplast controlled study darkness genetic transcription histone acetylation intron light mammal cell mutant nonhuman plant regulatory mechanism transcription elongation Light makes carbon fixation possible, allowing plant and animal life on Earth. We have previously shown that light regulates alternative splicing in plants. Light initiates a chloroplast retrograde signaling that regulates nuclear alternative splicing of a subset of Arabidopsis thaliana transcripts. Here, we show that light promotes RNA polymerase II (Pol II) elongation in the affected genes, whereas in darkness, elongation is lower. These changes in transcription are consistent with elongation causing the observed changes in alternative splicing, as revealed by different drug treatments and genetic evidence. The light control of splicing and elongation is abolished in an Arabidopsis mutant defective in the transcription factor IIS (TFIIS). We report that the chloroplast control of nuclear alternative splicing in plants responds to the kinetic coupling mechanism found in mammalian cells, providing unique evidence that coupling is important for a whole organism to respond to environmental cues. Godoy Herz et al. provide biochemical and genetic evidence that plants exposed to light show faster gene transcription than those in the dark. This serves as control for alternative mRNA splicing decisions, which demonstrates that coupling between transcription and splicing is important for a whole organism to respond to environmental cues. © 2018 Elsevier Inc. 2019 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10972765_v73_n5_p1066_GodoyHerz http://hdl.handle.net/20.500.12110/paper_10972765_v73_n5_p1066_GodoyHerz |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
alternative splicing light control in plants transcription elongation histone messenger RNA RNA polymerase II transcription factor II alternative RNA splicing Arabidopsis thaliana Article carbon fixation chloroplast controlled study darkness genetic transcription histone acetylation intron light mammal cell mutant nonhuman plant regulatory mechanism transcription elongation |
| spellingShingle |
alternative splicing light control in plants transcription elongation histone messenger RNA RNA polymerase II transcription factor II alternative RNA splicing Arabidopsis thaliana Article carbon fixation chloroplast controlled study darkness genetic transcription histone acetylation intron light mammal cell mutant nonhuman plant regulatory mechanism transcription elongation Light Regulates Plant Alternative Splicing through the Control of Transcriptional Elongation |
| topic_facet |
alternative splicing light control in plants transcription elongation histone messenger RNA RNA polymerase II transcription factor II alternative RNA splicing Arabidopsis thaliana Article carbon fixation chloroplast controlled study darkness genetic transcription histone acetylation intron light mammal cell mutant nonhuman plant regulatory mechanism transcription elongation |
| description |
Light makes carbon fixation possible, allowing plant and animal life on Earth. We have previously shown that light regulates alternative splicing in plants. Light initiates a chloroplast retrograde signaling that regulates nuclear alternative splicing of a subset of Arabidopsis thaliana transcripts. Here, we show that light promotes RNA polymerase II (Pol II) elongation in the affected genes, whereas in darkness, elongation is lower. These changes in transcription are consistent with elongation causing the observed changes in alternative splicing, as revealed by different drug treatments and genetic evidence. The light control of splicing and elongation is abolished in an Arabidopsis mutant defective in the transcription factor IIS (TFIIS). We report that the chloroplast control of nuclear alternative splicing in plants responds to the kinetic coupling mechanism found in mammalian cells, providing unique evidence that coupling is important for a whole organism to respond to environmental cues. Godoy Herz et al. provide biochemical and genetic evidence that plants exposed to light show faster gene transcription than those in the dark. This serves as control for alternative mRNA splicing decisions, which demonstrates that coupling between transcription and splicing is important for a whole organism to respond to environmental cues. © 2018 Elsevier Inc. |
| title |
Light Regulates Plant Alternative Splicing through the Control of Transcriptional Elongation |
| title_short |
Light Regulates Plant Alternative Splicing through the Control of Transcriptional Elongation |
| title_full |
Light Regulates Plant Alternative Splicing through the Control of Transcriptional Elongation |
| title_fullStr |
Light Regulates Plant Alternative Splicing through the Control of Transcriptional Elongation |
| title_full_unstemmed |
Light Regulates Plant Alternative Splicing through the Control of Transcriptional Elongation |
| title_sort |
light regulates plant alternative splicing through the control of transcriptional elongation |
| publishDate |
2019 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10972765_v73_n5_p1066_GodoyHerz http://hdl.handle.net/20.500.12110/paper_10972765_v73_n5_p1066_GodoyHerz |
| _version_ |
1768543764246167552 |