Coupling of transcription with alternative splicing: RNA pol II promoters modulate SF2/ASF and 9G8 effects on an exonic splicing enhancer

Alternative mRNA splicing of the fibronectin EDI exon is controlled by a purine-rich exonic splicing enhancer (ESE), postulated as a binding site for SR proteins. By using a transient expression alternative splicing assay combined with promoter swapping, we have demonstrated that the promoter can al...

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Detalles Bibliográficos
Publicado:	1999
Materias:	alpha globin DNA fibronectin messenger RNA Pol protein protein RNA transcription factor article binding site cell line enhancer region exon genetic linkage human molecular interaction RNA splicing transcription regulation
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10972765_v4_n2_p251_Cramer http://hdl.handle.net/20.500.12110/paper_10972765_v4_n2_p251_Cramer
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_10972765_v4_n2_p251_Cramer
record_format	dspace
spelling	paper:paper_10972765_v4_n2_p251_Cramer2023-06-08T16:07:07Z Coupling of transcription with alternative splicing: RNA pol II promoters modulate SF2/ASF and 9G8 effects on an exonic splicing enhancer alpha globin DNA fibronectin messenger RNA Pol protein protein RNA transcription factor article binding site cell line enhancer region exon genetic linkage human molecular interaction RNA splicing transcription regulation Alternative mRNA splicing of the fibronectin EDI exon is controlled by a purine-rich exonic splicing enhancer (ESE), postulated as a binding site for SR proteins. By using a transient expression alternative splicing assay combined with promoter swapping, we have demonstrated that the promoter can also control EDI splicing, arguing for coupling between the transcription and splicing machineries. We now report that the SR proteins SF2/ASF and 9G8 stimulate EDI splicing in vivo and that their effect requires an intact EDI ESE. Most importantly, we show that sensitivity to these SR proteins critically depends on the promoter structure, suggesting that the transcription machinery modulates their recruitment to the ESE. 1999 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10972765_v4_n2_p251_Cramer http://hdl.handle.net/20.500.12110/paper_10972765_v4_n2_p251_Cramer
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	alpha globin DNA fibronectin messenger RNA Pol protein protein RNA transcription factor article binding site cell line enhancer region exon genetic linkage human molecular interaction RNA splicing transcription regulation
spellingShingle	alpha globin DNA fibronectin messenger RNA Pol protein protein RNA transcription factor article binding site cell line enhancer region exon genetic linkage human molecular interaction RNA splicing transcription regulation Coupling of transcription with alternative splicing: RNA pol II promoters modulate SF2/ASF and 9G8 effects on an exonic splicing enhancer
topic_facet	alpha globin DNA fibronectin messenger RNA Pol protein protein RNA transcription factor article binding site cell line enhancer region exon genetic linkage human molecular interaction RNA splicing transcription regulation
description	Alternative mRNA splicing of the fibronectin EDI exon is controlled by a purine-rich exonic splicing enhancer (ESE), postulated as a binding site for SR proteins. By using a transient expression alternative splicing assay combined with promoter swapping, we have demonstrated that the promoter can also control EDI splicing, arguing for coupling between the transcription and splicing machineries. We now report that the SR proteins SF2/ASF and 9G8 stimulate EDI splicing in vivo and that their effect requires an intact EDI ESE. Most importantly, we show that sensitivity to these SR proteins critically depends on the promoter structure, suggesting that the transcription machinery modulates their recruitment to the ESE.
title	Coupling of transcription with alternative splicing: RNA pol II promoters modulate SF2/ASF and 9G8 effects on an exonic splicing enhancer
title_short	Coupling of transcription with alternative splicing: RNA pol II promoters modulate SF2/ASF and 9G8 effects on an exonic splicing enhancer
title_full	Coupling of transcription with alternative splicing: RNA pol II promoters modulate SF2/ASF and 9G8 effects on an exonic splicing enhancer
title_fullStr	Coupling of transcription with alternative splicing: RNA pol II promoters modulate SF2/ASF and 9G8 effects on an exonic splicing enhancer
title_full_unstemmed	Coupling of transcription with alternative splicing: RNA pol II promoters modulate SF2/ASF and 9G8 effects on an exonic splicing enhancer
title_sort	coupling of transcription with alternative splicing: rna pol ii promoters modulate sf2/asf and 9g8 effects on an exonic splicing enhancer
publishDate	1999
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10972765_v4_n2_p251_Cramer http://hdl.handle.net/20.500.12110/paper_10972765_v4_n2_p251_Cramer
_version_	1768543047955513344

Coupling of transcription with alternative splicing: RNA pol II promoters modulate SF2/ASF and 9G8 effects on an exonic splicing enhancer

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