The small GTP-binding protein RhoA regulates c-jun by a ROCK-JNK signaling axis
RhoA regulates the actin cytoskeleton and the expression of genes associated with cell proliferation. This includes c-fos and c-jun, which are members of the AP1 family of transcription factors that play a key role in normal and aberrant cell growth. Whereas RhoA stimulates the c-fos SRE by a recent...
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2004
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10972765_v14_n1_p29_Marinissen http://hdl.handle.net/20.500.12110/paper_10972765_v14_n1_p29_Marinissen |
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paper:paper_10972765_v14_n1_p29_Marinissen2023-06-08T16:07:06Z The small GTP-binding protein RhoA regulates c-jun by a ROCK-JNK signaling axis actin activating transcription factor 2 protein protein c fos protein c jun protein kinase protein Rock RhoA guanine nucleotide binding protein stress activated protein kinase transcription factor AP 1 unclassified drug animal cell article cell growth cell nucleus cell proliferation controlled study cytoskeleton downstream processing enzyme activation enzyme activity gene expression human human cell molecular mechanics mouse nonhuman polymerization promoter region protein binding protein expression protein family protein function protein phosphorylation regulatory mechanism serum responsive element signal transduction stimulation Actin Depolymerizing Factors Actins Activating Transcription Factor 2 Animals Cell Line Cyclic AMP Response Element-Binding Protein Cytoskeleton Enzyme Activation Enzyme Inhibitors Gene Expression Regulation Humans Intracellular Signaling Peptides and Proteins JNK Mitogen-Activated Protein Kinases Lysophospholipids Mice Microfilament Proteins Mitogen-Activated Protein Kinases NIH 3T3 Cells Promoter Regions (Genetics) Protein Kinases Protein-Serine-Threonine Kinases Proto-Oncogene Proteins c-jun rhoA GTP-Binding Protein Signal Transduction Transcription Factors Animalia RhoA regulates the actin cytoskeleton and the expression of genes associated with cell proliferation. This includes c-fos and c-jun, which are members of the AP1 family of transcription factors that play a key role in normal and aberrant cell growth. Whereas RhoA stimulates the c-fos SRE by a recently elucidated mechanism that is dependent on actin treadmilling, how RhoA regulates c-jun is still poorly understood. We found that RhoA stimulates c-jun expression through ROCK, but independently from the ability of ROCK to promote actin polymerization. Instead, we found that ROCK activates JNK, which then phosphorylates c-Jun and ATF2 when bound to the c-jun promoter. Thus, ROCK represents a point of signal divergence downstream from RhoA, as it promotes actin reorganization and the consequent expression from the c-fos SRE, while a parallel pathway connects ROCK to JNK, thereby stimulating c-jun expression. Ultimately, these pathways converge in the nucleus to regulate AP1 activity. 2004 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10972765_v14_n1_p29_Marinissen http://hdl.handle.net/20.500.12110/paper_10972765_v14_n1_p29_Marinissen |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
actin activating transcription factor 2 protein protein c fos protein c jun protein kinase protein Rock RhoA guanine nucleotide binding protein stress activated protein kinase transcription factor AP 1 unclassified drug animal cell article cell growth cell nucleus cell proliferation controlled study cytoskeleton downstream processing enzyme activation enzyme activity gene expression human human cell molecular mechanics mouse nonhuman polymerization promoter region protein binding protein expression protein family protein function protein phosphorylation regulatory mechanism serum responsive element signal transduction stimulation Actin Depolymerizing Factors Actins Activating Transcription Factor 2 Animals Cell Line Cyclic AMP Response Element-Binding Protein Cytoskeleton Enzyme Activation Enzyme Inhibitors Gene Expression Regulation Humans Intracellular Signaling Peptides and Proteins JNK Mitogen-Activated Protein Kinases Lysophospholipids Mice Microfilament Proteins Mitogen-Activated Protein Kinases NIH 3T3 Cells Promoter Regions (Genetics) Protein Kinases Protein-Serine-Threonine Kinases Proto-Oncogene Proteins c-jun rhoA GTP-Binding Protein Signal Transduction Transcription Factors Animalia |
| spellingShingle |
actin activating transcription factor 2 protein protein c fos protein c jun protein kinase protein Rock RhoA guanine nucleotide binding protein stress activated protein kinase transcription factor AP 1 unclassified drug animal cell article cell growth cell nucleus cell proliferation controlled study cytoskeleton downstream processing enzyme activation enzyme activity gene expression human human cell molecular mechanics mouse nonhuman polymerization promoter region protein binding protein expression protein family protein function protein phosphorylation regulatory mechanism serum responsive element signal transduction stimulation Actin Depolymerizing Factors Actins Activating Transcription Factor 2 Animals Cell Line Cyclic AMP Response Element-Binding Protein Cytoskeleton Enzyme Activation Enzyme Inhibitors Gene Expression Regulation Humans Intracellular Signaling Peptides and Proteins JNK Mitogen-Activated Protein Kinases Lysophospholipids Mice Microfilament Proteins Mitogen-Activated Protein Kinases NIH 3T3 Cells Promoter Regions (Genetics) Protein Kinases Protein-Serine-Threonine Kinases Proto-Oncogene Proteins c-jun rhoA GTP-Binding Protein Signal Transduction Transcription Factors Animalia The small GTP-binding protein RhoA regulates c-jun by a ROCK-JNK signaling axis |
| topic_facet |
actin activating transcription factor 2 protein protein c fos protein c jun protein kinase protein Rock RhoA guanine nucleotide binding protein stress activated protein kinase transcription factor AP 1 unclassified drug animal cell article cell growth cell nucleus cell proliferation controlled study cytoskeleton downstream processing enzyme activation enzyme activity gene expression human human cell molecular mechanics mouse nonhuman polymerization promoter region protein binding protein expression protein family protein function protein phosphorylation regulatory mechanism serum responsive element signal transduction stimulation Actin Depolymerizing Factors Actins Activating Transcription Factor 2 Animals Cell Line Cyclic AMP Response Element-Binding Protein Cytoskeleton Enzyme Activation Enzyme Inhibitors Gene Expression Regulation Humans Intracellular Signaling Peptides and Proteins JNK Mitogen-Activated Protein Kinases Lysophospholipids Mice Microfilament Proteins Mitogen-Activated Protein Kinases NIH 3T3 Cells Promoter Regions (Genetics) Protein Kinases Protein-Serine-Threonine Kinases Proto-Oncogene Proteins c-jun rhoA GTP-Binding Protein Signal Transduction Transcription Factors Animalia |
| description |
RhoA regulates the actin cytoskeleton and the expression of genes associated with cell proliferation. This includes c-fos and c-jun, which are members of the AP1 family of transcription factors that play a key role in normal and aberrant cell growth. Whereas RhoA stimulates the c-fos SRE by a recently elucidated mechanism that is dependent on actin treadmilling, how RhoA regulates c-jun is still poorly understood. We found that RhoA stimulates c-jun expression through ROCK, but independently from the ability of ROCK to promote actin polymerization. Instead, we found that ROCK activates JNK, which then phosphorylates c-Jun and ATF2 when bound to the c-jun promoter. Thus, ROCK represents a point of signal divergence downstream from RhoA, as it promotes actin reorganization and the consequent expression from the c-fos SRE, while a parallel pathway connects ROCK to JNK, thereby stimulating c-jun expression. Ultimately, these pathways converge in the nucleus to regulate AP1 activity. |
| title |
The small GTP-binding protein RhoA regulates c-jun by a ROCK-JNK signaling axis |
| title_short |
The small GTP-binding protein RhoA regulates c-jun by a ROCK-JNK signaling axis |
| title_full |
The small GTP-binding protein RhoA regulates c-jun by a ROCK-JNK signaling axis |
| title_fullStr |
The small GTP-binding protein RhoA regulates c-jun by a ROCK-JNK signaling axis |
| title_full_unstemmed |
The small GTP-binding protein RhoA regulates c-jun by a ROCK-JNK signaling axis |
| title_sort |
small gtp-binding protein rhoa regulates c-jun by a rock-jnk signaling axis |
| publishDate |
2004 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10972765_v14_n1_p29_Marinissen http://hdl.handle.net/20.500.12110/paper_10972765_v14_n1_p29_Marinissen |
| _version_ |
1768541950213881856 |