Possible role of carbonic anhydrase, V-H+-ATPase, and Cl -/HCO3 - exchanger in electrogenic ion transport across the gills of the euryhaline crab Chasmagnathus granulatus
We studied the participation of carbonic anhydrase (CA), V-H +-ATPase, and Cl-/HCO3 - exchanger in electrogenic ion absorption through the gills of Chasmagnathus granulatus. CA activity was measured in anterior gills and posterior gills after acclimation to 2‰, 10‰, 30‰ (about seawater), and 45‰ sal...
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2005
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10956433_v142_n3_p362_Genovese http://hdl.handle.net/20.500.12110/paper_10956433_v142_n3_p362_Genovese |
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paper:paper_10956433_v142_n3_p362_Genovese2023-06-08T16:07:01Z Possible role of carbonic anhydrase, V-H+-ATPase, and Cl -/HCO3 - exchanger in electrogenic ion transport across the gills of the euryhaline crab Chasmagnathus granulatus Genovese, Griselda Ortiz, Nicolás Luquet, Carlos Marcelo Carbonic anhydrase Chasmagnathus granulatus Cl-/HCO3 - exchanger Crab Electrogenic ion transport Gills H+-ATPase Transepithelial potential difference 4 acetamido 4' isothiocyanatostilbene 2,2' disulfonic acid acetazolamide adenosine triphosphatase bafilomycin A1 carbonate dehydratase ouabain absorption acclimatization animal tissue article controlled study crab cytosol enzyme activity gill ion exchange ion transport microsome nonhuman osmosis perfusion salinity Acetazolamide Adenosine Triphosphatases Animals Biological Transport, Active Brachyura Carbonic Anhydrase Inhibitors Carbonic Anhydrases Chloride-Bicarbonate Antiporters Electrophysiology Gills Ion Transport Osmotic Pressure Ouabain Sodium Chloride Animalia Chasmagnathus granulata Decapoda (Crustacea) Strophanthus gratus We studied the participation of carbonic anhydrase (CA), V-H +-ATPase, and Cl-/HCO3 - exchanger in electrogenic ion absorption through the gills of Chasmagnathus granulatus. CA activity was measured in anterior gills and posterior gills after acclimation to 2‰, 10‰, 30‰ (about seawater), and 45‰ salinity. The highest CA specific activity was detected in the microsomal fraction in anterior gills, and in the cytosolic fraction, in posterior ones. Both fractions were strongly induced by decreasing salinity only in posterior gills. Perfusion of posterior gills from crabs acclimated to either 2‰ or 10‰ with acetazolamide inhibited CA activity almost completely. In posterior gills from crabs acclimated to 2‰ and perfused with 20‰ saline (iso-osmotic for these crabs), acetazolamide reduced transepithelial potential difference (Vte) by 47%, further addition of ouabain enhanced the effect to 88%. Acetazolamide had no effect in the same gills perfused with 30‰ saline (iso-osmotic for seawater acclimated crabs). Bafilomycin A1 and SITS (inhibitors of V-H+-ATPase and Cl-/HCO3 -) reduced Vte by 15-16% in gills perfused with normal 20‰ saline, and by 77% and 45%, respectively when they were applied in Na-free 20‰ saline, suggesting the participation of those transporters and cytosolic CA in electrogenic ion absorption. © 2005 Elsevier Inc. All rights reserved. Fil:Genovese, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ortiz, N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Luquet, C.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2005 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10956433_v142_n3_p362_Genovese http://hdl.handle.net/20.500.12110/paper_10956433_v142_n3_p362_Genovese |
| institution |
Universidad de Buenos Aires |
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I-28 |
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R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Carbonic anhydrase Chasmagnathus granulatus Cl-/HCO3 - exchanger Crab Electrogenic ion transport Gills H+-ATPase Transepithelial potential difference 4 acetamido 4' isothiocyanatostilbene 2,2' disulfonic acid acetazolamide adenosine triphosphatase bafilomycin A1 carbonate dehydratase ouabain absorption acclimatization animal tissue article controlled study crab cytosol enzyme activity gill ion exchange ion transport microsome nonhuman osmosis perfusion salinity Acetazolamide Adenosine Triphosphatases Animals Biological Transport, Active Brachyura Carbonic Anhydrase Inhibitors Carbonic Anhydrases Chloride-Bicarbonate Antiporters Electrophysiology Gills Ion Transport Osmotic Pressure Ouabain Sodium Chloride Animalia Chasmagnathus granulata Decapoda (Crustacea) Strophanthus gratus |
| spellingShingle |
Carbonic anhydrase Chasmagnathus granulatus Cl-/HCO3 - exchanger Crab Electrogenic ion transport Gills H+-ATPase Transepithelial potential difference 4 acetamido 4' isothiocyanatostilbene 2,2' disulfonic acid acetazolamide adenosine triphosphatase bafilomycin A1 carbonate dehydratase ouabain absorption acclimatization animal tissue article controlled study crab cytosol enzyme activity gill ion exchange ion transport microsome nonhuman osmosis perfusion salinity Acetazolamide Adenosine Triphosphatases Animals Biological Transport, Active Brachyura Carbonic Anhydrase Inhibitors Carbonic Anhydrases Chloride-Bicarbonate Antiporters Electrophysiology Gills Ion Transport Osmotic Pressure Ouabain Sodium Chloride Animalia Chasmagnathus granulata Decapoda (Crustacea) Strophanthus gratus Genovese, Griselda Ortiz, Nicolás Luquet, Carlos Marcelo Possible role of carbonic anhydrase, V-H+-ATPase, and Cl -/HCO3 - exchanger in electrogenic ion transport across the gills of the euryhaline crab Chasmagnathus granulatus |
| topic_facet |
Carbonic anhydrase Chasmagnathus granulatus Cl-/HCO3 - exchanger Crab Electrogenic ion transport Gills H+-ATPase Transepithelial potential difference 4 acetamido 4' isothiocyanatostilbene 2,2' disulfonic acid acetazolamide adenosine triphosphatase bafilomycin A1 carbonate dehydratase ouabain absorption acclimatization animal tissue article controlled study crab cytosol enzyme activity gill ion exchange ion transport microsome nonhuman osmosis perfusion salinity Acetazolamide Adenosine Triphosphatases Animals Biological Transport, Active Brachyura Carbonic Anhydrase Inhibitors Carbonic Anhydrases Chloride-Bicarbonate Antiporters Electrophysiology Gills Ion Transport Osmotic Pressure Ouabain Sodium Chloride Animalia Chasmagnathus granulata Decapoda (Crustacea) Strophanthus gratus |
| description |
We studied the participation of carbonic anhydrase (CA), V-H +-ATPase, and Cl-/HCO3 - exchanger in electrogenic ion absorption through the gills of Chasmagnathus granulatus. CA activity was measured in anterior gills and posterior gills after acclimation to 2‰, 10‰, 30‰ (about seawater), and 45‰ salinity. The highest CA specific activity was detected in the microsomal fraction in anterior gills, and in the cytosolic fraction, in posterior ones. Both fractions were strongly induced by decreasing salinity only in posterior gills. Perfusion of posterior gills from crabs acclimated to either 2‰ or 10‰ with acetazolamide inhibited CA activity almost completely. In posterior gills from crabs acclimated to 2‰ and perfused with 20‰ saline (iso-osmotic for these crabs), acetazolamide reduced transepithelial potential difference (Vte) by 47%, further addition of ouabain enhanced the effect to 88%. Acetazolamide had no effect in the same gills perfused with 30‰ saline (iso-osmotic for seawater acclimated crabs). Bafilomycin A1 and SITS (inhibitors of V-H+-ATPase and Cl-/HCO3 -) reduced Vte by 15-16% in gills perfused with normal 20‰ saline, and by 77% and 45%, respectively when they were applied in Na-free 20‰ saline, suggesting the participation of those transporters and cytosolic CA in electrogenic ion absorption. © 2005 Elsevier Inc. All rights reserved. |
| author |
Genovese, Griselda Ortiz, Nicolás Luquet, Carlos Marcelo |
| author_facet |
Genovese, Griselda Ortiz, Nicolás Luquet, Carlos Marcelo |
| author_sort |
Genovese, Griselda |
| title |
Possible role of carbonic anhydrase, V-H+-ATPase, and Cl -/HCO3 - exchanger in electrogenic ion transport across the gills of the euryhaline crab Chasmagnathus granulatus |
| title_short |
Possible role of carbonic anhydrase, V-H+-ATPase, and Cl -/HCO3 - exchanger in electrogenic ion transport across the gills of the euryhaline crab Chasmagnathus granulatus |
| title_full |
Possible role of carbonic anhydrase, V-H+-ATPase, and Cl -/HCO3 - exchanger in electrogenic ion transport across the gills of the euryhaline crab Chasmagnathus granulatus |
| title_fullStr |
Possible role of carbonic anhydrase, V-H+-ATPase, and Cl -/HCO3 - exchanger in electrogenic ion transport across the gills of the euryhaline crab Chasmagnathus granulatus |
| title_full_unstemmed |
Possible role of carbonic anhydrase, V-H+-ATPase, and Cl -/HCO3 - exchanger in electrogenic ion transport across the gills of the euryhaline crab Chasmagnathus granulatus |
| title_sort |
possible role of carbonic anhydrase, v-h+-atpase, and cl -/hco3 - exchanger in electrogenic ion transport across the gills of the euryhaline crab chasmagnathus granulatus |
| publishDate |
2005 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10956433_v142_n3_p362_Genovese http://hdl.handle.net/20.500.12110/paper_10956433_v142_n3_p362_Genovese |
| work_keys_str_mv |
AT genovesegriselda possibleroleofcarbonicanhydrasevhatpaseandclhco3exchangerinelectrogeniciontransportacrossthegillsoftheeuryhalinecrabchasmagnathusgranulatus AT ortiznicolas possibleroleofcarbonicanhydrasevhatpaseandclhco3exchangerinelectrogeniciontransportacrossthegillsoftheeuryhalinecrabchasmagnathusgranulatus AT luquetcarlosmarcelo possibleroleofcarbonicanhydrasevhatpaseandclhco3exchangerinelectrogeniciontransportacrossthegillsoftheeuryhalinecrabchasmagnathusgranulatus |
| _version_ |
1768543047758381056 |