Glyphosate commercial formulation causes cytotoxicity, oxidative effects, and apoptosis on human cells: Differences with its active ingredient

In the present study, the effects on oxidative balance and cellular end points of glyphosate, aminomethylphosphonic acid (AMPA), and a glyphosate formulation (G formulation) were examined in HepG2 cell line, at dilution levels far below agricultural recommendations. Our results show that G formulati...

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Autores principales:	Chaufan, Rosa Gabriela, Coalova, Isis, Ríos de Molina, María del Carmen
Publicado:	2014
Materias:	apoptosis cytotoxicity glyphosate in vtro oxidative stress Adjuvants, Pharmaceutic Apoptosis Chemistry, Pharmaceutical Drug Synergism Glutathione Glycine Hep G2 Cells Hepatocytes Herbicides Humans Lethal Dose 50 Organophosphonates Osmolar Concentration Oxidation-Reduction Oxidative Stress Reactive Oxygen Species Superoxide Dismutase Tyrosine Up-Regulation
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10915818_v33_n1_p29_Chaufan http://hdl.handle.net/20.500.12110/paper_10915818_v33_n1_p29_Chaufan
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_10915818_v33_n1_p29_Chaufan
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spelling	paper:paper_10915818_v33_n1_p29_Chaufan2023-06-08T16:06:42Z Glyphosate commercial formulation causes cytotoxicity, oxidative effects, and apoptosis on human cells: Differences with its active ingredient Chaufan, Rosa Gabriela Coalova, Isis Ríos de Molina, María del Carmen apoptosis cytotoxicity glyphosate in vtro oxidative stress apoptosis cytotoxicity glyphosate in vtro oxidative stress Adjuvants, Pharmaceutic Apoptosis Chemistry, Pharmaceutical Drug Synergism Glutathione Glycine Hep G2 Cells Hepatocytes Herbicides Humans Lethal Dose 50 Organophosphonates Osmolar Concentration Oxidation-Reduction Oxidative Stress Reactive Oxygen Species Superoxide Dismutase Tyrosine Up-Regulation In the present study, the effects on oxidative balance and cellular end points of glyphosate, aminomethylphosphonic acid (AMPA), and a glyphosate formulation (G formulation) were examined in HepG2 cell line, at dilution levels far below agricultural recommendations. Our results show that G formulation had toxic effects while no effects were found with acid glyphosate and AMPA treatments. Glyphosate formulation exposure produced an increase in reactive oxygen species, nitrotyrosine formation, superoxide dismutase activity, and glutathione (GSH) levels, while no effects were observed for catalase and GSH-S-transferase activities. Also, G formulation triggered caspase 3/7 activation and hence induced apoptosis pathway in this cell line. Aminomethylphosphonic acid exposure produced an increase in GSH levels while no differences were observed in other antioxidant parameters. No effects were observed when the cells were exposed to acid glyphosate. These results confirm that G formulations have adjuvants working together with the active ingredient and causing toxic effects that are not seen with acid glyphosate. © The Author(s) 2014. Fil:Chaufan, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Coalova, I. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Del Carmen Ríos De Molina, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2014 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10915818_v33_n1_p29_Chaufan http://hdl.handle.net/20.500.12110/paper_10915818_v33_n1_p29_Chaufan
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	apoptosis cytotoxicity glyphosate in vtro oxidative stress apoptosis cytotoxicity glyphosate in vtro oxidative stress Adjuvants, Pharmaceutic Apoptosis Chemistry, Pharmaceutical Drug Synergism Glutathione Glycine Hep G2 Cells Hepatocytes Herbicides Humans Lethal Dose 50 Organophosphonates Osmolar Concentration Oxidation-Reduction Oxidative Stress Reactive Oxygen Species Superoxide Dismutase Tyrosine Up-Regulation
spellingShingle	apoptosis cytotoxicity glyphosate in vtro oxidative stress apoptosis cytotoxicity glyphosate in vtro oxidative stress Adjuvants, Pharmaceutic Apoptosis Chemistry, Pharmaceutical Drug Synergism Glutathione Glycine Hep G2 Cells Hepatocytes Herbicides Humans Lethal Dose 50 Organophosphonates Osmolar Concentration Oxidation-Reduction Oxidative Stress Reactive Oxygen Species Superoxide Dismutase Tyrosine Up-Regulation Chaufan, Rosa Gabriela Coalova, Isis Ríos de Molina, María del Carmen Glyphosate commercial formulation causes cytotoxicity, oxidative effects, and apoptosis on human cells: Differences with its active ingredient
topic_facet	apoptosis cytotoxicity glyphosate in vtro oxidative stress apoptosis cytotoxicity glyphosate in vtro oxidative stress Adjuvants, Pharmaceutic Apoptosis Chemistry, Pharmaceutical Drug Synergism Glutathione Glycine Hep G2 Cells Hepatocytes Herbicides Humans Lethal Dose 50 Organophosphonates Osmolar Concentration Oxidation-Reduction Oxidative Stress Reactive Oxygen Species Superoxide Dismutase Tyrosine Up-Regulation
description	In the present study, the effects on oxidative balance and cellular end points of glyphosate, aminomethylphosphonic acid (AMPA), and a glyphosate formulation (G formulation) were examined in HepG2 cell line, at dilution levels far below agricultural recommendations. Our results show that G formulation had toxic effects while no effects were found with acid glyphosate and AMPA treatments. Glyphosate formulation exposure produced an increase in reactive oxygen species, nitrotyrosine formation, superoxide dismutase activity, and glutathione (GSH) levels, while no effects were observed for catalase and GSH-S-transferase activities. Also, G formulation triggered caspase 3/7 activation and hence induced apoptosis pathway in this cell line. Aminomethylphosphonic acid exposure produced an increase in GSH levels while no differences were observed in other antioxidant parameters. No effects were observed when the cells were exposed to acid glyphosate. These results confirm that G formulations have adjuvants working together with the active ingredient and causing toxic effects that are not seen with acid glyphosate. © The Author(s) 2014.
author	Chaufan, Rosa Gabriela Coalova, Isis Ríos de Molina, María del Carmen
author_facet	Chaufan, Rosa Gabriela Coalova, Isis Ríos de Molina, María del Carmen
author_sort	Chaufan, Rosa Gabriela
title	Glyphosate commercial formulation causes cytotoxicity, oxidative effects, and apoptosis on human cells: Differences with its active ingredient
title_short	Glyphosate commercial formulation causes cytotoxicity, oxidative effects, and apoptosis on human cells: Differences with its active ingredient
title_full	Glyphosate commercial formulation causes cytotoxicity, oxidative effects, and apoptosis on human cells: Differences with its active ingredient
title_fullStr	Glyphosate commercial formulation causes cytotoxicity, oxidative effects, and apoptosis on human cells: Differences with its active ingredient
title_full_unstemmed	Glyphosate commercial formulation causes cytotoxicity, oxidative effects, and apoptosis on human cells: Differences with its active ingredient
title_sort	glyphosate commercial formulation causes cytotoxicity, oxidative effects, and apoptosis on human cells: differences with its active ingredient
publishDate	2014
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10915818_v33_n1_p29_Chaufan http://hdl.handle.net/20.500.12110/paper_10915818_v33_n1_p29_Chaufan
work_keys_str_mv	AT chaufanrosagabriela glyphosatecommercialformulationcausescytotoxicityoxidativeeffectsandapoptosisonhumancellsdifferenceswithitsactiveingredient AT coalovaisis glyphosatecommercialformulationcausescytotoxicityoxidativeeffectsandapoptosisonhumancellsdifferenceswithitsactiveingredient AT riosdemolinamariadelcarmen glyphosatecommercialformulationcausescytotoxicityoxidativeeffectsandapoptosisonhumancellsdifferenceswithitsactiveingredient
_version_	1768545429648048128

Glyphosate commercial formulation causes cytotoxicity, oxidative effects, and apoptosis on human cells: Differences with its active ingredient

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