Nitric oxide sets off an antioxidant response in adrenal cells: Involvement of sGC and Nrf2 in HO-1 induction

Induction of microsomal heme oxygenase 1 (HO-1) activity is considered a cytoprotective mechanism in different cell types. In adrenal cells, HO-1 induction by ACTH exerts a modulatory effect on steroid production as well. As nitric oxide (NO) has been also regarded as an autocrine/paracrine modulato...

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Autores principales: Astort, Francisco, Degese, Maria Sol, Coso, Omar Adrian, Cymeryng, Cora Beatriz
Publicado: 2014
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Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10898603_v37_n1_p1_Astort
http://hdl.handle.net/20.500.12110/paper_10898603_v37_n1_p1_Astort
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spelling paper:paper_10898603_v37_n1_p1_Astort2023-06-08T16:06:40Z Nitric oxide sets off an antioxidant response in adrenal cells: Involvement of sGC and Nrf2 in HO-1 induction Astort, Francisco Degese, Maria Sol Coso, Omar Adrian Cymeryng, Cora Beatriz cGMP Heme oxygenase 1 Nitric oxide Nuclear factor-erythroid 2-related factor (Nrf2) Soluble guanylate cyclase cyclic GMP dactinomycin diethylenetriamine guanylate cyclase guanylate cyclase inhibitor heme oxygenase 1 messenger RNA nitric oxide transcription factor Nrf2 antioxidant guanylate cyclase heme oxygenase 1 Nfe2l2 protein, mouse nitric oxide transcription factor Nrf2 adrenal cortex cell animal cell article cellular stress response controlled study enzyme induction intracellular signaling mouse nonhuman oxidative stress priority journal protein localization regulatory mechanism RNA degradation transcription initiation transient transfection adrenal gland animal cell culture cytology enzymology metabolism solubility Adrenal Glands Animals Antioxidants Cells, Cultured Guanylate Cyclase Heme Oxygenase-1 Mice NF-E2-Related Factor 2 Nitric Oxide Solubility Induction of microsomal heme oxygenase 1 (HO-1) activity is considered a cytoprotective mechanism in different cell types. In adrenal cells, HO-1 induction by ACTH exerts a modulatory effect on steroid production as well. As nitric oxide (NO) has been also regarded as an autocrine/paracrine modulator of adrenal steroidogenesis we sought to study the effects of NO on the induction of HO-1 and the mechanism involved. We hereby analyzed the time and dose-dependent effect of a NO-donor (DETA/NO) on HO-1 induction in a murine adrenocortical cell line. We showed that this effect is mainly exerted at a transcriptional level as it is inhibited by actinomycin D and HO-1 mRNA degradation rates were not affected by DETA/NO treatment. HO-1 induction by NO does not appear to involve the generation of oxidative stress as it was not affected by antioxidant treatment. We also demonstrated that NO-treatment results in the nuclear translocation of the nuclear factor-erythroid 2-related factor (Nrf2), an effect that is attenuated by transfecting the cells with a dominant negative isoform of Nrf2. We finally show that the effects of the NO-donor are reproduced by a permeable analog of cGMP and that a soluble guanylate cyclase specific inhibitor blocked both the induction of HO-1 by NO and the nuclear translocation of Nrf2. © 2013 Elsevier Inc. All rights reserved. Fil:Astort, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Degese, M.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Coso, O. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cymeryng, C.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2014 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10898603_v37_n1_p1_Astort http://hdl.handle.net/20.500.12110/paper_10898603_v37_n1_p1_Astort
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic cGMP
Heme oxygenase 1
Nitric oxide
Nuclear factor-erythroid 2-related factor (Nrf2)
Soluble guanylate cyclase
cyclic GMP
dactinomycin
diethylenetriamine
guanylate cyclase
guanylate cyclase inhibitor
heme oxygenase 1
messenger RNA
nitric oxide
transcription factor Nrf2
antioxidant
guanylate cyclase
heme oxygenase 1
Nfe2l2 protein, mouse
nitric oxide
transcription factor Nrf2
adrenal cortex cell
animal cell
article
cellular stress response
controlled study
enzyme induction
intracellular signaling
mouse
nonhuman
oxidative stress
priority journal
protein localization
regulatory mechanism
RNA degradation
transcription initiation
transient transfection
adrenal gland
animal
cell culture
cytology
enzymology
metabolism
solubility
Adrenal Glands
Animals
Antioxidants
Cells, Cultured
Guanylate Cyclase
Heme Oxygenase-1
Mice
NF-E2-Related Factor 2
Nitric Oxide
Solubility
spellingShingle cGMP
Heme oxygenase 1
Nitric oxide
Nuclear factor-erythroid 2-related factor (Nrf2)
Soluble guanylate cyclase
cyclic GMP
dactinomycin
diethylenetriamine
guanylate cyclase
guanylate cyclase inhibitor
heme oxygenase 1
messenger RNA
nitric oxide
transcription factor Nrf2
antioxidant
guanylate cyclase
heme oxygenase 1
Nfe2l2 protein, mouse
nitric oxide
transcription factor Nrf2
adrenal cortex cell
animal cell
article
cellular stress response
controlled study
enzyme induction
intracellular signaling
mouse
nonhuman
oxidative stress
priority journal
protein localization
regulatory mechanism
RNA degradation
transcription initiation
transient transfection
adrenal gland
animal
cell culture
cytology
enzymology
metabolism
solubility
Adrenal Glands
Animals
Antioxidants
Cells, Cultured
Guanylate Cyclase
Heme Oxygenase-1
Mice
NF-E2-Related Factor 2
Nitric Oxide
Solubility
Astort, Francisco
Degese, Maria Sol
Coso, Omar Adrian
Cymeryng, Cora Beatriz
Nitric oxide sets off an antioxidant response in adrenal cells: Involvement of sGC and Nrf2 in HO-1 induction
topic_facet cGMP
Heme oxygenase 1
Nitric oxide
Nuclear factor-erythroid 2-related factor (Nrf2)
Soluble guanylate cyclase
cyclic GMP
dactinomycin
diethylenetriamine
guanylate cyclase
guanylate cyclase inhibitor
heme oxygenase 1
messenger RNA
nitric oxide
transcription factor Nrf2
antioxidant
guanylate cyclase
heme oxygenase 1
Nfe2l2 protein, mouse
nitric oxide
transcription factor Nrf2
adrenal cortex cell
animal cell
article
cellular stress response
controlled study
enzyme induction
intracellular signaling
mouse
nonhuman
oxidative stress
priority journal
protein localization
regulatory mechanism
RNA degradation
transcription initiation
transient transfection
adrenal gland
animal
cell culture
cytology
enzymology
metabolism
solubility
Adrenal Glands
Animals
Antioxidants
Cells, Cultured
Guanylate Cyclase
Heme Oxygenase-1
Mice
NF-E2-Related Factor 2
Nitric Oxide
Solubility
description Induction of microsomal heme oxygenase 1 (HO-1) activity is considered a cytoprotective mechanism in different cell types. In adrenal cells, HO-1 induction by ACTH exerts a modulatory effect on steroid production as well. As nitric oxide (NO) has been also regarded as an autocrine/paracrine modulator of adrenal steroidogenesis we sought to study the effects of NO on the induction of HO-1 and the mechanism involved. We hereby analyzed the time and dose-dependent effect of a NO-donor (DETA/NO) on HO-1 induction in a murine adrenocortical cell line. We showed that this effect is mainly exerted at a transcriptional level as it is inhibited by actinomycin D and HO-1 mRNA degradation rates were not affected by DETA/NO treatment. HO-1 induction by NO does not appear to involve the generation of oxidative stress as it was not affected by antioxidant treatment. We also demonstrated that NO-treatment results in the nuclear translocation of the nuclear factor-erythroid 2-related factor (Nrf2), an effect that is attenuated by transfecting the cells with a dominant negative isoform of Nrf2. We finally show that the effects of the NO-donor are reproduced by a permeable analog of cGMP and that a soluble guanylate cyclase specific inhibitor blocked both the induction of HO-1 by NO and the nuclear translocation of Nrf2. © 2013 Elsevier Inc. All rights reserved.
author Astort, Francisco
Degese, Maria Sol
Coso, Omar Adrian
Cymeryng, Cora Beatriz
author_facet Astort, Francisco
Degese, Maria Sol
Coso, Omar Adrian
Cymeryng, Cora Beatriz
author_sort Astort, Francisco
title Nitric oxide sets off an antioxidant response in adrenal cells: Involvement of sGC and Nrf2 in HO-1 induction
title_short Nitric oxide sets off an antioxidant response in adrenal cells: Involvement of sGC and Nrf2 in HO-1 induction
title_full Nitric oxide sets off an antioxidant response in adrenal cells: Involvement of sGC and Nrf2 in HO-1 induction
title_fullStr Nitric oxide sets off an antioxidant response in adrenal cells: Involvement of sGC and Nrf2 in HO-1 induction
title_full_unstemmed Nitric oxide sets off an antioxidant response in adrenal cells: Involvement of sGC and Nrf2 in HO-1 induction
title_sort nitric oxide sets off an antioxidant response in adrenal cells: involvement of sgc and nrf2 in ho-1 induction
publishDate 2014
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10898603_v37_n1_p1_Astort
http://hdl.handle.net/20.500.12110/paper_10898603_v37_n1_p1_Astort
work_keys_str_mv AT astortfrancisco nitricoxidesetsoffanantioxidantresponseinadrenalcellsinvolvementofsgcandnrf2inho1induction
AT degesemariasol nitricoxidesetsoffanantioxidantresponseinadrenalcellsinvolvementofsgcandnrf2inho1induction
AT cosoomaradrian nitricoxidesetsoffanantioxidantresponseinadrenalcellsinvolvementofsgcandnrf2inho1induction
AT cymeryngcorabeatriz nitricoxidesetsoffanantioxidantresponseinadrenalcellsinvolvementofsgcandnrf2inho1induction
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