Novel long non-protein coding RNAs involved in Arabidopsis differentiation and stress responses

Long non-protein coding RNAs (npcRNA) represent an emerging class of riboregulators, which either act directly in this long form or are processed to shorter miRNA and siRNA. Genome-wide bioinformatic analysis of full-length cDNA databases identified 76 Arabidopsis npcRNAs. Fourteen npcRNAs were anti...

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Guardado en:
Detalles Bibliográficos
Autores principales: Wirth, Sonia Alejandra, Crespi, Martín Darío
Publicado: 2009
Materias:
plant RNA
RNA binding protein
transcriptome
Arabidopsis protein
cell cycle protein
DCL1 protein, Arabidopsis
DCL4 protein, Arabidopsis
microRNA
ribonuclease
ribonuclease III
RNA precursor
small interfering RNA
untranslated RNA
abiotic stress
Arabidopsis
article
bioinformatics
cell differentiation
gene overexpression
nonhuman
plant genetics
plant growth
plant stress
priority journal
RNA analysis
DNA microarray
gene expression profiling
genetics
growth, development and aging
molecular genetics
mutation
nucleotide sequence
physiological stress
physiology
plant gene
transgenic plant
Arabidopsis Proteins
Base Sequence
Cell Cycle Proteins
Gene Expression Profiling
Genes, Plant
MicroRNAs
Molecular Sequence Data
Mutation
Oligonucleotide Array Sequence Analysis
Plants, Genetically Modified
Ribonuclease III
Ribonucleases
RNA Precursors
RNA, Plant
RNA, Small Interfering
RNA, Untranslated
Stress, Physiological
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10889051_v19_n1_p57_Amor
http://hdl.handle.net/20.500.12110/paper_10889051_v19_n1_p57_Amor
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:Long non-protein coding RNAs (npcRNA) represent an emerging class of riboregulators, which either act directly in this long form or are processed to shorter miRNA and siRNA. Genome-wide bioinformatic analysis of full-length cDNA databases identified 76 Arabidopsis npcRNAs. Fourteen npcRNAs were antisense to protein-coding mRNAs, suggesting cis-regulatory roles. Numerous 24-nt siRNA matched to five different npcRNAs, suggesting that these npcRNAs are precursors of this type of siRNA. Expression analyses of the 76 npcRNAs identified a novel npcRNA that accumulates in a dcll mutant but does not appear to produce trans-acting siRNA or miRNA. Additionally, another npcRNA was the precursor of miR869 and shown to be up-regulated in dcl4 but not in dcll mutants, indicative of a young miRNA gene. Abiotic stress altered the accumulation of 22 npcRNAs among the 76, a fraction significantly higher than that observed for the RNA binding protein-coding fraction of the transcriptome. Overexpression analyses in Arabidopsis identified two npcRNAs as regulators of root growth during salt stress and leaf morphology, respectively. Hence, together with small RNAs, long npcRNAs encompass a sensitive component of the transcriptome that have diverse roles during growth and differentiation.

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