Novel long non-protein coding RNAs involved in Arabidopsis differentiation and stress responses

Long non-protein coding RNAs (npcRNA) represent an emerging class of riboregulators, which either act directly in this long form or are processed to shorter miRNA and siRNA. Genome-wide bioinformatic analysis of full-length cDNA databases identified 76 Arabidopsis npcRNAs. Fourteen npcRNAs were anti...

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Autores principales:	Wirth, Sonia Alejandra, Crespi, Martín Darío
Publicado:	2009
Materias:	plant RNA RNA binding protein transcriptome Arabidopsis protein cell cycle protein DCL1 protein, Arabidopsis DCL4 protein, Arabidopsis microRNA ribonuclease ribonuclease III RNA precursor small interfering RNA untranslated RNA abiotic stress Arabidopsis article bioinformatics cell differentiation gene overexpression nonhuman plant genetics plant growth plant stress priority journal RNA analysis DNA microarray gene expression profiling genetics growth, development and aging molecular genetics mutation nucleotide sequence physiological stress physiology plant gene transgenic plant Arabidopsis Proteins Base Sequence Cell Cycle Proteins Gene Expression Profiling Genes, Plant MicroRNAs Molecular Sequence Data Mutation Oligonucleotide Array Sequence Analysis Plants, Genetically Modified Ribonuclease III Ribonucleases RNA Precursors RNA, Plant RNA, Small Interfering RNA, Untranslated Stress, Physiological
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10889051_v19_n1_p57_Amor http://hdl.handle.net/20.500.12110/paper_10889051_v19_n1_p57_Amor
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_10889051_v19_n1_p57_Amor
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spelling	paper:paper_10889051_v19_n1_p57_Amor2023-06-08T16:06:13Z Novel long non-protein coding RNAs involved in Arabidopsis differentiation and stress responses Wirth, Sonia Alejandra Crespi, Martín Darío plant RNA RNA binding protein transcriptome Arabidopsis protein cell cycle protein DCL1 protein, Arabidopsis DCL4 protein, Arabidopsis microRNA plant RNA ribonuclease ribonuclease III RNA precursor small interfering RNA untranslated RNA abiotic stress Arabidopsis article bioinformatics cell differentiation gene overexpression nonhuman plant genetics plant growth plant stress priority journal RNA analysis DNA microarray gene expression profiling genetics growth, development and aging molecular genetics mutation nucleotide sequence physiological stress physiology plant gene transgenic plant Arabidopsis Arabidopsis Arabidopsis Proteins Base Sequence Cell Cycle Proteins Gene Expression Profiling Genes, Plant MicroRNAs Molecular Sequence Data Mutation Oligonucleotide Array Sequence Analysis Plants, Genetically Modified Ribonuclease III Ribonucleases RNA Precursors RNA, Plant RNA, Small Interfering RNA, Untranslated Stress, Physiological Long non-protein coding RNAs (npcRNA) represent an emerging class of riboregulators, which either act directly in this long form or are processed to shorter miRNA and siRNA. Genome-wide bioinformatic analysis of full-length cDNA databases identified 76 Arabidopsis npcRNAs. Fourteen npcRNAs were antisense to protein-coding mRNAs, suggesting cis-regulatory roles. Numerous 24-nt siRNA matched to five different npcRNAs, suggesting that these npcRNAs are precursors of this type of siRNA. Expression analyses of the 76 npcRNAs identified a novel npcRNA that accumulates in a dcll mutant but does not appear to produce trans-acting siRNA or miRNA. Additionally, another npcRNA was the precursor of miR869 and shown to be up-regulated in dcl4 but not in dcll mutants, indicative of a young miRNA gene. Abiotic stress altered the accumulation of 22 npcRNAs among the 76, a fraction significantly higher than that observed for the RNA binding protein-coding fraction of the transcriptome. Overexpression analyses in Arabidopsis identified two npcRNAs as regulators of root growth during salt stress and leaf morphology, respectively. Hence, together with small RNAs, long npcRNAs encompass a sensitive component of the transcriptome that have diverse roles during growth and differentiation. Fil:Wirth, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Crespi, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2009 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10889051_v19_n1_p57_Amor http://hdl.handle.net/20.500.12110/paper_10889051_v19_n1_p57_Amor
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	plant RNA RNA binding protein transcriptome Arabidopsis protein cell cycle protein DCL1 protein, Arabidopsis DCL4 protein, Arabidopsis microRNA plant RNA ribonuclease ribonuclease III RNA precursor small interfering RNA untranslated RNA abiotic stress Arabidopsis article bioinformatics cell differentiation gene overexpression nonhuman plant genetics plant growth plant stress priority journal RNA analysis DNA microarray gene expression profiling genetics growth, development and aging molecular genetics mutation nucleotide sequence physiological stress physiology plant gene transgenic plant Arabidopsis Arabidopsis Arabidopsis Proteins Base Sequence Cell Cycle Proteins Gene Expression Profiling Genes, Plant MicroRNAs Molecular Sequence Data Mutation Oligonucleotide Array Sequence Analysis Plants, Genetically Modified Ribonuclease III Ribonucleases RNA Precursors RNA, Plant RNA, Small Interfering RNA, Untranslated Stress, Physiological
spellingShingle	plant RNA RNA binding protein transcriptome Arabidopsis protein cell cycle protein DCL1 protein, Arabidopsis DCL4 protein, Arabidopsis microRNA plant RNA ribonuclease ribonuclease III RNA precursor small interfering RNA untranslated RNA abiotic stress Arabidopsis article bioinformatics cell differentiation gene overexpression nonhuman plant genetics plant growth plant stress priority journal RNA analysis DNA microarray gene expression profiling genetics growth, development and aging molecular genetics mutation nucleotide sequence physiological stress physiology plant gene transgenic plant Arabidopsis Arabidopsis Arabidopsis Proteins Base Sequence Cell Cycle Proteins Gene Expression Profiling Genes, Plant MicroRNAs Molecular Sequence Data Mutation Oligonucleotide Array Sequence Analysis Plants, Genetically Modified Ribonuclease III Ribonucleases RNA Precursors RNA, Plant RNA, Small Interfering RNA, Untranslated Stress, Physiological Wirth, Sonia Alejandra Crespi, Martín Darío Novel long non-protein coding RNAs involved in Arabidopsis differentiation and stress responses
topic_facet	plant RNA RNA binding protein transcriptome Arabidopsis protein cell cycle protein DCL1 protein, Arabidopsis DCL4 protein, Arabidopsis microRNA plant RNA ribonuclease ribonuclease III RNA precursor small interfering RNA untranslated RNA abiotic stress Arabidopsis article bioinformatics cell differentiation gene overexpression nonhuman plant genetics plant growth plant stress priority journal RNA analysis DNA microarray gene expression profiling genetics growth, development and aging molecular genetics mutation nucleotide sequence physiological stress physiology plant gene transgenic plant Arabidopsis Arabidopsis Arabidopsis Proteins Base Sequence Cell Cycle Proteins Gene Expression Profiling Genes, Plant MicroRNAs Molecular Sequence Data Mutation Oligonucleotide Array Sequence Analysis Plants, Genetically Modified Ribonuclease III Ribonucleases RNA Precursors RNA, Plant RNA, Small Interfering RNA, Untranslated Stress, Physiological
description	Long non-protein coding RNAs (npcRNA) represent an emerging class of riboregulators, which either act directly in this long form or are processed to shorter miRNA and siRNA. Genome-wide bioinformatic analysis of full-length cDNA databases identified 76 Arabidopsis npcRNAs. Fourteen npcRNAs were antisense to protein-coding mRNAs, suggesting cis-regulatory roles. Numerous 24-nt siRNA matched to five different npcRNAs, suggesting that these npcRNAs are precursors of this type of siRNA. Expression analyses of the 76 npcRNAs identified a novel npcRNA that accumulates in a dcll mutant but does not appear to produce trans-acting siRNA or miRNA. Additionally, another npcRNA was the precursor of miR869 and shown to be up-regulated in dcl4 but not in dcll mutants, indicative of a young miRNA gene. Abiotic stress altered the accumulation of 22 npcRNAs among the 76, a fraction significantly higher than that observed for the RNA binding protein-coding fraction of the transcriptome. Overexpression analyses in Arabidopsis identified two npcRNAs as regulators of root growth during salt stress and leaf morphology, respectively. Hence, together with small RNAs, long npcRNAs encompass a sensitive component of the transcriptome that have diverse roles during growth and differentiation.
author	Wirth, Sonia Alejandra Crespi, Martín Darío
author_facet	Wirth, Sonia Alejandra Crespi, Martín Darío
author_sort	Wirth, Sonia Alejandra
title	Novel long non-protein coding RNAs involved in Arabidopsis differentiation and stress responses
title_short	Novel long non-protein coding RNAs involved in Arabidopsis differentiation and stress responses
title_full	Novel long non-protein coding RNAs involved in Arabidopsis differentiation and stress responses
title_fullStr	Novel long non-protein coding RNAs involved in Arabidopsis differentiation and stress responses
title_full_unstemmed	Novel long non-protein coding RNAs involved in Arabidopsis differentiation and stress responses
title_sort	novel long non-protein coding rnas involved in arabidopsis differentiation and stress responses
publishDate	2009
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10889051_v19_n1_p57_Amor http://hdl.handle.net/20.500.12110/paper_10889051_v19_n1_p57_Amor
work_keys_str_mv	AT wirthsoniaalejandra novellongnonproteincodingrnasinvolvedinarabidopsisdifferentiationandstressresponses AT crespimartindario novellongnonproteincodingrnasinvolvedinarabidopsisdifferentiationandstressresponses
_version_	1768545018055753728

Novel long non-protein coding RNAs involved in Arabidopsis differentiation and stress responses

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