Exploring dynamics in living cells by tracking single particles

In the last years, significant advances in microscopy techniques and the introduction of a novel technology to label living cells with genetically encoded fluorescent proteins revolutionized the field of Cell Biology. Our understanding on cell dynamics built from snapshots on fixed specimens has evo...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autor principal:	Levi, Valeria
Publicado:	2007
Materias:	Dynamics Intracellular motion Single particle tracking Tracking algorithm Trajectory fluorescent dye molecular motor photoprotein quantum dot algorithm biomechanics biophysics cell function confocal microscopy fluorescence microscopy fluorescence recovery after photobleaching methodology movement (physiology) physiology review spectrofluorometry statistics Algorithms Biomechanics Biophysics Cell Physiology Fluorescence Recovery After Photobleaching Fluorescent Dyes Luminescent Proteins Microscopy, Confocal Microscopy, Fluorescence Molecular Motor Proteins Movement Quantum Dots Spectrometry, Fluorescence
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10859195_v48_n1_p1_Levi http://hdl.handle.net/20.500.12110/paper_10859195_v48_n1_p1_Levi
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_10859195_v48_n1_p1_Levi
record_format	dspace
spelling	paper:paper_10859195_v48_n1_p1_Levi2023-06-08T16:06:07Z Exploring dynamics in living cells by tracking single particles Levi, Valeria Dynamics Intracellular motion Single particle tracking Tracking algorithm Trajectory fluorescent dye molecular motor photoprotein quantum dot algorithm biomechanics biophysics cell function confocal microscopy fluorescence microscopy fluorescence recovery after photobleaching methodology movement (physiology) physiology review spectrofluorometry statistics Algorithms Biomechanics Biophysics Cell Physiology Fluorescence Recovery After Photobleaching Fluorescent Dyes Luminescent Proteins Microscopy, Confocal Microscopy, Fluorescence Molecular Motor Proteins Movement Quantum Dots Spectrometry, Fluorescence In the last years, significant advances in microscopy techniques and the introduction of a novel technology to label living cells with genetically encoded fluorescent proteins revolutionized the field of Cell Biology. Our understanding on cell dynamics built from snapshots on fixed specimens has evolved thanks to our actual capability to monitor in real time the evolution of processes in living cells. Among these new tools, single particle tracking techniques were developed to observe and follow individual particles. Hence, we are starting to unravel the mechanisms driving the motion of a wide variety of cellular components ranging from organelles to protein molecules by following their way through the cell. In this review, we introduce the single particle tracking technology to new users. We briefly describe the instrumentation and explain some of the algorithms commonly used to locate and track particles. Also, we present some common tools used to analyze trajectories and illustrate with some examples the applications of single particle tracking to study dynamics in living cells. © Humana Press Inc. 2007. Fil:Levi, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2007 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10859195_v48_n1_p1_Levi http://hdl.handle.net/20.500.12110/paper_10859195_v48_n1_p1_Levi
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Dynamics Intracellular motion Single particle tracking Tracking algorithm Trajectory fluorescent dye molecular motor photoprotein quantum dot algorithm biomechanics biophysics cell function confocal microscopy fluorescence microscopy fluorescence recovery after photobleaching methodology movement (physiology) physiology review spectrofluorometry statistics Algorithms Biomechanics Biophysics Cell Physiology Fluorescence Recovery After Photobleaching Fluorescent Dyes Luminescent Proteins Microscopy, Confocal Microscopy, Fluorescence Molecular Motor Proteins Movement Quantum Dots Spectrometry, Fluorescence
spellingShingle	Dynamics Intracellular motion Single particle tracking Tracking algorithm Trajectory fluorescent dye molecular motor photoprotein quantum dot algorithm biomechanics biophysics cell function confocal microscopy fluorescence microscopy fluorescence recovery after photobleaching methodology movement (physiology) physiology review spectrofluorometry statistics Algorithms Biomechanics Biophysics Cell Physiology Fluorescence Recovery After Photobleaching Fluorescent Dyes Luminescent Proteins Microscopy, Confocal Microscopy, Fluorescence Molecular Motor Proteins Movement Quantum Dots Spectrometry, Fluorescence Levi, Valeria Exploring dynamics in living cells by tracking single particles
topic_facet	Dynamics Intracellular motion Single particle tracking Tracking algorithm Trajectory fluorescent dye molecular motor photoprotein quantum dot algorithm biomechanics biophysics cell function confocal microscopy fluorescence microscopy fluorescence recovery after photobleaching methodology movement (physiology) physiology review spectrofluorometry statistics Algorithms Biomechanics Biophysics Cell Physiology Fluorescence Recovery After Photobleaching Fluorescent Dyes Luminescent Proteins Microscopy, Confocal Microscopy, Fluorescence Molecular Motor Proteins Movement Quantum Dots Spectrometry, Fluorescence
description	In the last years, significant advances in microscopy techniques and the introduction of a novel technology to label living cells with genetically encoded fluorescent proteins revolutionized the field of Cell Biology. Our understanding on cell dynamics built from snapshots on fixed specimens has evolved thanks to our actual capability to monitor in real time the evolution of processes in living cells. Among these new tools, single particle tracking techniques were developed to observe and follow individual particles. Hence, we are starting to unravel the mechanisms driving the motion of a wide variety of cellular components ranging from organelles to protein molecules by following their way through the cell. In this review, we introduce the single particle tracking technology to new users. We briefly describe the instrumentation and explain some of the algorithms commonly used to locate and track particles. Also, we present some common tools used to analyze trajectories and illustrate with some examples the applications of single particle tracking to study dynamics in living cells. © Humana Press Inc. 2007.
author	Levi, Valeria
author_facet	Levi, Valeria
author_sort	Levi, Valeria
title	Exploring dynamics in living cells by tracking single particles
title_short	Exploring dynamics in living cells by tracking single particles
title_full	Exploring dynamics in living cells by tracking single particles
title_fullStr	Exploring dynamics in living cells by tracking single particles
title_full_unstemmed	Exploring dynamics in living cells by tracking single particles
title_sort	exploring dynamics in living cells by tracking single particles
publishDate	2007
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10859195_v48_n1_p1_Levi http://hdl.handle.net/20.500.12110/paper_10859195_v48_n1_p1_Levi
work_keys_str_mv	AT levivaleria exploringdynamicsinlivingcellsbytrackingsingleparticles
_version_	1768545705163489280

Exploring dynamics in living cells by tracking single particles

Ejemplares similares