Suppression of SPARC expression by antisense RNA abrogates the tumorigenicity of human melanoma cells

Acquisition of invasive/metastatic potential is a key event in tumor progression. Cell surface glycoproteins and their respective matrix ligands have been implicated in this process. Recent evidence reveals that the secreted glycoprotein SPARC (secreted protein, acidic and rich in cysteine) is highl...

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Autores principales:	Ledda, María Fernanda, Bover, Laura del Carmen, Podhajcer, Osvaldo Luis
Publicado:	1997
Materias:	complementary rna glycoprotein osteonectin animal experiment article cancer inhibition cancer invasion controlled study human human cell melanoma cell metastasis mouse nonhuman priority journal Animals Cell Adhesion Cell Division Cell Movement Down-Regulation Gene Expression Regulation, Neoplastic Humans Melanoma Melanoma, Experimental Mice Oligonucleotides, Antisense Osteonectin Transfection Tumor Cells, Cultured Animalia
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10788956_v3_n2_p171_FernandaLedda http://hdl.handle.net/20.500.12110/paper_10788956_v3_n2_p171_FernandaLedda
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_10788956_v3_n2_p171_FernandaLedda
record_format	dspace
spelling	paper:paper_10788956_v3_n2_p171_FernandaLedda2023-06-08T16:05:36Z Suppression of SPARC expression by antisense RNA abrogates the tumorigenicity of human melanoma cells Ledda, María Fernanda Bover, Laura del Carmen Podhajcer, Osvaldo Luis complementary rna glycoprotein osteonectin animal experiment article cancer inhibition cancer invasion controlled study human human cell melanoma cell metastasis mouse nonhuman priority journal Animals Cell Adhesion Cell Division Cell Movement Down-Regulation Gene Expression Regulation, Neoplastic Humans Melanoma Melanoma, Experimental Mice Oligonucleotides, Antisense Osteonectin Transfection Tumor Cells, Cultured Animalia Acquisition of invasive/metastatic potential is a key event in tumor progression. Cell surface glycoproteins and their respective matrix ligands have been implicated in this process. Recent evidence reveals that the secreted glycoprotein SPARC (secreted protein, acidic and rich in cysteine) is highly expressed in different malignant tissues. The present study reports that the suppression of SPARC expression by human melanoma cells using a SPARC antisense expression vector results in a significant decrease in the in vitro adhesive and invasive capacities of tumor cells, completely abolishing their in vivo tumorigenicity. This is the first evidence that SPARC plays a key role in human melanoma invasive-metastatic phenotype development. Fil:Fernanda Ledda, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Bover, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Podhajcer, O.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1997 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10788956_v3_n2_p171_FernandaLedda http://hdl.handle.net/20.500.12110/paper_10788956_v3_n2_p171_FernandaLedda
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	complementary rna glycoprotein osteonectin animal experiment article cancer inhibition cancer invasion controlled study human human cell melanoma cell metastasis mouse nonhuman priority journal Animals Cell Adhesion Cell Division Cell Movement Down-Regulation Gene Expression Regulation, Neoplastic Humans Melanoma Melanoma, Experimental Mice Oligonucleotides, Antisense Osteonectin Transfection Tumor Cells, Cultured Animalia
spellingShingle	complementary rna glycoprotein osteonectin animal experiment article cancer inhibition cancer invasion controlled study human human cell melanoma cell metastasis mouse nonhuman priority journal Animals Cell Adhesion Cell Division Cell Movement Down-Regulation Gene Expression Regulation, Neoplastic Humans Melanoma Melanoma, Experimental Mice Oligonucleotides, Antisense Osteonectin Transfection Tumor Cells, Cultured Animalia Ledda, María Fernanda Bover, Laura del Carmen Podhajcer, Osvaldo Luis Suppression of SPARC expression by antisense RNA abrogates the tumorigenicity of human melanoma cells
topic_facet	complementary rna glycoprotein osteonectin animal experiment article cancer inhibition cancer invasion controlled study human human cell melanoma cell metastasis mouse nonhuman priority journal Animals Cell Adhesion Cell Division Cell Movement Down-Regulation Gene Expression Regulation, Neoplastic Humans Melanoma Melanoma, Experimental Mice Oligonucleotides, Antisense Osteonectin Transfection Tumor Cells, Cultured Animalia
description	Acquisition of invasive/metastatic potential is a key event in tumor progression. Cell surface glycoproteins and their respective matrix ligands have been implicated in this process. Recent evidence reveals that the secreted glycoprotein SPARC (secreted protein, acidic and rich in cysteine) is highly expressed in different malignant tissues. The present study reports that the suppression of SPARC expression by human melanoma cells using a SPARC antisense expression vector results in a significant decrease in the in vitro adhesive and invasive capacities of tumor cells, completely abolishing their in vivo tumorigenicity. This is the first evidence that SPARC plays a key role in human melanoma invasive-metastatic phenotype development.
author	Ledda, María Fernanda Bover, Laura del Carmen Podhajcer, Osvaldo Luis
author_facet	Ledda, María Fernanda Bover, Laura del Carmen Podhajcer, Osvaldo Luis
author_sort	Ledda, María Fernanda
title	Suppression of SPARC expression by antisense RNA abrogates the tumorigenicity of human melanoma cells
title_short	Suppression of SPARC expression by antisense RNA abrogates the tumorigenicity of human melanoma cells
title_full	Suppression of SPARC expression by antisense RNA abrogates the tumorigenicity of human melanoma cells
title_fullStr	Suppression of SPARC expression by antisense RNA abrogates the tumorigenicity of human melanoma cells
title_full_unstemmed	Suppression of SPARC expression by antisense RNA abrogates the tumorigenicity of human melanoma cells
title_sort	suppression of sparc expression by antisense rna abrogates the tumorigenicity of human melanoma cells
publishDate	1997
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10788956_v3_n2_p171_FernandaLedda http://hdl.handle.net/20.500.12110/paper_10788956_v3_n2_p171_FernandaLedda
work_keys_str_mv	AT leddamariafernanda suppressionofsparcexpressionbyantisensernaabrogatesthetumorigenicityofhumanmelanomacells AT boverlauradelcarmen suppressionofsparcexpressionbyantisensernaabrogatesthetumorigenicityofhumanmelanomacells AT podhajcerosvaldoluis suppressionofsparcexpressionbyantisensernaabrogatesthetumorigenicityofhumanmelanomacells
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Suppression of SPARC expression by antisense RNA abrogates the tumorigenicity of human melanoma cells

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