Prostate tumor growth is impaired by CtBP1 depletion in high-fat diet-fed mice
Purpose: Clinical and epidemiologic data suggest that obesity is associated with more aggressive forms of prostate cancer, poor prognosis, and increased mortality. C-terminal-binding protein 1 (CtBP1) is a transcription repressor of tumor suppressor genes and is activated by NADH binding. High calor...
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2014
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10780432_v20_n15_p4086_Moiola http://hdl.handle.net/20.500.12110/paper_10780432_v20_n15_p4086_Moiola |
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paper:paper_10780432_v20_n15_p4086_Moiola |
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dspace |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
carboxy terminal binding protein 1 tumor suppressor protein unclassified drug alcohol dehydrogenase C-terminal binding protein DNA binding protein messenger RNA sex hormone small interfering RNA tumor marker animal cell animal experiment animal model animal tissue article cancer inhibition cell adhesion cell cycle controlled study gene expression gene identification genetic analysis in vitro study in vivo study lipid diet male metabolism mouse nonhuman priority journal prostate cancer protein depletion protein function adverse effects animal antagonists and inhibitors apoptosis cell proliferation cell transformation chromatin immunoprecipitation DNA microarray drug effects drug screening enzyme immunoassay gene expression profiling genetics human lipid diet metabolic syndrome X nude mouse obesity pathology Prostatic Neoplasms real time polymerase chain reaction reverse transcription polymerase chain reaction tumor cell culture Western blotting Alcohol Oxidoreductases Animals Apoptosis Blotting, Western Cell Adhesion Cell Proliferation Cell Transformation, Neoplastic Chromatin Immunoprecipitation Diet, High-Fat DNA-Binding Proteins Gene Expression Profiling Gonadal Steroid Hormones Humans Immunoenzyme Techniques Male Metabolic Syndrome X Mice Mice, Nude Obesity Oligonucleotide Array Sequence Analysis Prostatic Neoplasms Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger RNA, Small Interfering Tumor Cells, Cultured Tumor Markers, Biological Xenograft Model Antitumor Assays |
| spellingShingle |
carboxy terminal binding protein 1 tumor suppressor protein unclassified drug alcohol dehydrogenase C-terminal binding protein DNA binding protein messenger RNA sex hormone small interfering RNA tumor marker animal cell animal experiment animal model animal tissue article cancer inhibition cell adhesion cell cycle controlled study gene expression gene identification genetic analysis in vitro study in vivo study lipid diet male metabolism mouse nonhuman priority journal prostate cancer protein depletion protein function adverse effects animal antagonists and inhibitors apoptosis cell proliferation cell transformation chromatin immunoprecipitation DNA microarray drug effects drug screening enzyme immunoassay gene expression profiling genetics human lipid diet metabolic syndrome X nude mouse obesity pathology Prostatic Neoplasms real time polymerase chain reaction reverse transcription polymerase chain reaction tumor cell culture Western blotting Alcohol Oxidoreductases Animals Apoptosis Blotting, Western Cell Adhesion Cell Proliferation Cell Transformation, Neoplastic Chromatin Immunoprecipitation Diet, High-Fat DNA-Binding Proteins Gene Expression Profiling Gonadal Steroid Hormones Humans Immunoenzyme Techniques Male Metabolic Syndrome X Mice Mice, Nude Obesity Oligonucleotide Array Sequence Analysis Prostatic Neoplasms Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger RNA, Small Interfering Tumor Cells, Cultured Tumor Markers, Biological Xenograft Model Antitumor Assays Moiola, Cristian Pablo Rodriguez Segui, Santiago Andres Pignataro, Omar Pedro Vázquez, Elba Susana De Siervi, Adriana Prostate tumor growth is impaired by CtBP1 depletion in high-fat diet-fed mice |
| topic_facet |
carboxy terminal binding protein 1 tumor suppressor protein unclassified drug alcohol dehydrogenase C-terminal binding protein DNA binding protein messenger RNA sex hormone small interfering RNA tumor marker animal cell animal experiment animal model animal tissue article cancer inhibition cell adhesion cell cycle controlled study gene expression gene identification genetic analysis in vitro study in vivo study lipid diet male metabolism mouse nonhuman priority journal prostate cancer protein depletion protein function adverse effects animal antagonists and inhibitors apoptosis cell proliferation cell transformation chromatin immunoprecipitation DNA microarray drug effects drug screening enzyme immunoassay gene expression profiling genetics human lipid diet metabolic syndrome X nude mouse obesity pathology Prostatic Neoplasms real time polymerase chain reaction reverse transcription polymerase chain reaction tumor cell culture Western blotting Alcohol Oxidoreductases Animals Apoptosis Blotting, Western Cell Adhesion Cell Proliferation Cell Transformation, Neoplastic Chromatin Immunoprecipitation Diet, High-Fat DNA-Binding Proteins Gene Expression Profiling Gonadal Steroid Hormones Humans Immunoenzyme Techniques Male Metabolic Syndrome X Mice Mice, Nude Obesity Oligonucleotide Array Sequence Analysis Prostatic Neoplasms Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger RNA, Small Interfering Tumor Cells, Cultured Tumor Markers, Biological Xenograft Model Antitumor Assays |
| description |
Purpose: Clinical and epidemiologic data suggest that obesity is associated with more aggressive forms of prostate cancer, poor prognosis, and increased mortality. C-terminal-binding protein 1 (CtBP1) is a transcription repressor of tumor suppressor genes and is activated by NADH binding. High calorie intake decreases intracellular NAD+/NADH ratio. The aim of this work was to assess the effect of high-fat diet (HFD) and CtBP1 expression modulation over prostate xenograft growth. Experimental Design: We developed a metabolic syndrome-like disease in vivo model by feeding male nude mice with HFD during 16 weeks. Control diet (CD)-fed animals were maintained at the same conditions. Mice were inoculated with PC3 cells stable transfected with shCtBP1 or control plasmids. Genome-wide expression profiles and Gene Set Enrichment Analysis (GSEA) were performed from PC3. shCtBP1 versus PC3.pGIPZ HFD-fed mice tumors. Results: No significant differences were observed in tumor growth on CD-fed mice; however, we found that only 60% of HFD-fed mice inoculated with CtBP1-depleted cells developed a tumor. Moreover these tumors were significantly smaller than those generated by PC3.pGIPZ control xenografts. We found 823 genes differentially expressed in shCtBP1 tumors from HFD-fed mice. GSEA from expression dataset showed that most of these genes correspond to cell adhesion, metabolic process, and cell cycle. Conclusions: Metabolic syndrome-like diseases and CtBP1 expression cooperate to induce prostate tumor growth. Hence, targeting of CtBP1 expression might be considered for prostate cancer management and therapy in the subset of patients with metabolic syndromes. © 2014 American Association for Cancer Research. |
| author |
Moiola, Cristian Pablo Rodriguez Segui, Santiago Andres Pignataro, Omar Pedro Vázquez, Elba Susana De Siervi, Adriana |
| author_facet |
Moiola, Cristian Pablo Rodriguez Segui, Santiago Andres Pignataro, Omar Pedro Vázquez, Elba Susana De Siervi, Adriana |
| author_sort |
Moiola, Cristian Pablo |
| title |
Prostate tumor growth is impaired by CtBP1 depletion in high-fat diet-fed mice |
| title_short |
Prostate tumor growth is impaired by CtBP1 depletion in high-fat diet-fed mice |
| title_full |
Prostate tumor growth is impaired by CtBP1 depletion in high-fat diet-fed mice |
| title_fullStr |
Prostate tumor growth is impaired by CtBP1 depletion in high-fat diet-fed mice |
| title_full_unstemmed |
Prostate tumor growth is impaired by CtBP1 depletion in high-fat diet-fed mice |
| title_sort |
prostate tumor growth is impaired by ctbp1 depletion in high-fat diet-fed mice |
| publishDate |
2014 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10780432_v20_n15_p4086_Moiola http://hdl.handle.net/20.500.12110/paper_10780432_v20_n15_p4086_Moiola |
| work_keys_str_mv |
AT moiolacristianpablo prostatetumorgrowthisimpairedbyctbp1depletioninhighfatdietfedmice AT rodriguezseguisantiagoandres prostatetumorgrowthisimpairedbyctbp1depletioninhighfatdietfedmice AT pignataroomarpedro prostatetumorgrowthisimpairedbyctbp1depletioninhighfatdietfedmice AT vazquezelbasusana prostatetumorgrowthisimpairedbyctbp1depletioninhighfatdietfedmice AT desierviadriana prostatetumorgrowthisimpairedbyctbp1depletioninhighfatdietfedmice |
| _version_ |
1768542999641325568 |
| spelling |
paper:paper_10780432_v20_n15_p4086_Moiola2023-06-08T16:05:32Z Prostate tumor growth is impaired by CtBP1 depletion in high-fat diet-fed mice Moiola, Cristian Pablo Rodriguez Segui, Santiago Andres Pignataro, Omar Pedro Vázquez, Elba Susana De Siervi, Adriana carboxy terminal binding protein 1 tumor suppressor protein unclassified drug alcohol dehydrogenase C-terminal binding protein DNA binding protein messenger RNA sex hormone small interfering RNA tumor marker animal cell animal experiment animal model animal tissue article cancer inhibition cell adhesion cell cycle controlled study gene expression gene identification genetic analysis in vitro study in vivo study lipid diet male metabolism mouse nonhuman priority journal prostate cancer protein depletion protein function adverse effects animal antagonists and inhibitors apoptosis cell proliferation cell transformation chromatin immunoprecipitation DNA microarray drug effects drug screening enzyme immunoassay gene expression profiling genetics human lipid diet metabolic syndrome X nude mouse obesity pathology Prostatic Neoplasms real time polymerase chain reaction reverse transcription polymerase chain reaction tumor cell culture Western blotting Alcohol Oxidoreductases Animals Apoptosis Blotting, Western Cell Adhesion Cell Proliferation Cell Transformation, Neoplastic Chromatin Immunoprecipitation Diet, High-Fat DNA-Binding Proteins Gene Expression Profiling Gonadal Steroid Hormones Humans Immunoenzyme Techniques Male Metabolic Syndrome X Mice Mice, Nude Obesity Oligonucleotide Array Sequence Analysis Prostatic Neoplasms Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger RNA, Small Interfering Tumor Cells, Cultured Tumor Markers, Biological Xenograft Model Antitumor Assays Purpose: Clinical and epidemiologic data suggest that obesity is associated with more aggressive forms of prostate cancer, poor prognosis, and increased mortality. C-terminal-binding protein 1 (CtBP1) is a transcription repressor of tumor suppressor genes and is activated by NADH binding. High calorie intake decreases intracellular NAD+/NADH ratio. The aim of this work was to assess the effect of high-fat diet (HFD) and CtBP1 expression modulation over prostate xenograft growth. Experimental Design: We developed a metabolic syndrome-like disease in vivo model by feeding male nude mice with HFD during 16 weeks. Control diet (CD)-fed animals were maintained at the same conditions. Mice were inoculated with PC3 cells stable transfected with shCtBP1 or control plasmids. Genome-wide expression profiles and Gene Set Enrichment Analysis (GSEA) were performed from PC3. shCtBP1 versus PC3.pGIPZ HFD-fed mice tumors. Results: No significant differences were observed in tumor growth on CD-fed mice; however, we found that only 60% of HFD-fed mice inoculated with CtBP1-depleted cells developed a tumor. Moreover these tumors were significantly smaller than those generated by PC3.pGIPZ control xenografts. We found 823 genes differentially expressed in shCtBP1 tumors from HFD-fed mice. GSEA from expression dataset showed that most of these genes correspond to cell adhesion, metabolic process, and cell cycle. Conclusions: Metabolic syndrome-like diseases and CtBP1 expression cooperate to induce prostate tumor growth. Hence, targeting of CtBP1 expression might be considered for prostate cancer management and therapy in the subset of patients with metabolic syndromes. © 2014 American Association for Cancer Research. Fil:Moiola, C.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rodríguez-Seguí, S.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pignataro, O. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Vazquez, E.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:De Siervi, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2014 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10780432_v20_n15_p4086_Moiola http://hdl.handle.net/20.500.12110/paper_10780432_v20_n15_p4086_Moiola |