Detection of protein from detergent solutions by probe electrospray ionization mass spectrometry (PESI-MS)

Detergents are necessarily used for different extraction protocols of proteins from biological cells or tissues. After the extraction, elimination of detergent is necessary for the better performance of electrospray ionization mass spectrometry (ESI-MS). Elimination of detergents is laborious and ti...

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Publicado:	2011
Materias:	detergents electrospray multiply charged protein ions nano-electrospray probe electrospray ionization Analytical tool Biological cells Charged proteins Detergent solution Direct analysis Electrospray ionization mass spectrometry Electrosprays Extraction protocols Nano-ESI Protein extract Sample solution Sodium dodecyl sulphate Triton X-100 Bromine compounds Ionization Mass spectrometry Probes Proteins Soaps (detergents) Tissue Electrospray ionization 3 [(3 cholamidopropyl)dimethylammonio] 1 propanesulfonic acid cetrimonium chloride detergent dodecyl sulfate sodium triton x 100 article controlled study electrospray mass spectrometry nanoanalysis priority journal protein analysis Cetrimonium Compounds Detergents Equipment Design Octoxynol Sodium Dodecyl Sulfate Solutions Spectrometry, Mass, Electrospray Ionization
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10765174_v46_n10_p967_Mandal http://hdl.handle.net/20.500.12110/paper_10765174_v46_n10_p967_Mandal
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_10765174_v46_n10_p967_Mandal
record_format	dspace
spelling	paper:paper_10765174_v46_n10_p967_Mandal2023-06-08T16:05:23Z Detection of protein from detergent solutions by probe electrospray ionization mass spectrometry (PESI-MS) detergents electrospray multiply charged protein ions nano-electrospray probe electrospray ionization Analytical tool Biological cells Charged proteins Detergent solution Direct analysis Electrospray ionization mass spectrometry Electrosprays Extraction protocols nano-electrospray Nano-ESI Protein extract Sample solution Sodium dodecyl sulphate Triton X-100 Bromine compounds Ionization Mass spectrometry Probes Proteins Soaps (detergents) Tissue Electrospray ionization 3 [(3 cholamidopropyl)dimethylammonio] 1 propanesulfonic acid cetrimonium chloride detergent dodecyl sulfate sodium triton x 100 article controlled study electrospray electrospray mass spectrometry nanoanalysis priority journal protein analysis Cetrimonium Compounds Detergents Equipment Design Octoxynol Proteins Sodium Dodecyl Sulfate Solutions Spectrometry, Mass, Electrospray Ionization Detergents are necessarily used for different extraction protocols of proteins from biological cells or tissues. After the extraction, elimination of detergent is necessary for the better performance of electrospray ionization mass spectrometry (ESI-MS). Elimination of detergents is laborious and time-consuming, and also sample loss may be unavoidable. Probe electrospray ionization (PESI) developed in our laboratory has been found to be tolerant to the presence of salts and buffers in sample solutions. In this report, it was examined whether PESI is applicable to the sample solutions that contain high-concentration of detergents. It was found that PESI is highly tolerant to the presence of sodium dodecyl sulphate, cetyl trimethylamminium bromide, Triton X100 and 3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate compared with conventional ESI and nanoESI. Therefore, PESI can be a potential analytical tool for direct analysis of protein extracts and digests containing high-concentration detergents. Copyright © 2011 John Wiley & Sons, Ltd. 2011 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10765174_v46_n10_p967_Mandal http://hdl.handle.net/20.500.12110/paper_10765174_v46_n10_p967_Mandal
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	detergents electrospray multiply charged protein ions nano-electrospray probe electrospray ionization Analytical tool Biological cells Charged proteins Detergent solution Direct analysis Electrospray ionization mass spectrometry Electrosprays Extraction protocols nano-electrospray Nano-ESI Protein extract Sample solution Sodium dodecyl sulphate Triton X-100 Bromine compounds Ionization Mass spectrometry Probes Proteins Soaps (detergents) Tissue Electrospray ionization 3 [(3 cholamidopropyl)dimethylammonio] 1 propanesulfonic acid cetrimonium chloride detergent dodecyl sulfate sodium triton x 100 article controlled study electrospray electrospray mass spectrometry nanoanalysis priority journal protein analysis Cetrimonium Compounds Detergents Equipment Design Octoxynol Proteins Sodium Dodecyl Sulfate Solutions Spectrometry, Mass, Electrospray Ionization
spellingShingle	detergents electrospray multiply charged protein ions nano-electrospray probe electrospray ionization Analytical tool Biological cells Charged proteins Detergent solution Direct analysis Electrospray ionization mass spectrometry Electrosprays Extraction protocols nano-electrospray Nano-ESI Protein extract Sample solution Sodium dodecyl sulphate Triton X-100 Bromine compounds Ionization Mass spectrometry Probes Proteins Soaps (detergents) Tissue Electrospray ionization 3 [(3 cholamidopropyl)dimethylammonio] 1 propanesulfonic acid cetrimonium chloride detergent dodecyl sulfate sodium triton x 100 article controlled study electrospray electrospray mass spectrometry nanoanalysis priority journal protein analysis Cetrimonium Compounds Detergents Equipment Design Octoxynol Proteins Sodium Dodecyl Sulfate Solutions Spectrometry, Mass, Electrospray Ionization Detection of protein from detergent solutions by probe electrospray ionization mass spectrometry (PESI-MS)
topic_facet	detergents electrospray multiply charged protein ions nano-electrospray probe electrospray ionization Analytical tool Biological cells Charged proteins Detergent solution Direct analysis Electrospray ionization mass spectrometry Electrosprays Extraction protocols nano-electrospray Nano-ESI Protein extract Sample solution Sodium dodecyl sulphate Triton X-100 Bromine compounds Ionization Mass spectrometry Probes Proteins Soaps (detergents) Tissue Electrospray ionization 3 [(3 cholamidopropyl)dimethylammonio] 1 propanesulfonic acid cetrimonium chloride detergent dodecyl sulfate sodium triton x 100 article controlled study electrospray electrospray mass spectrometry nanoanalysis priority journal protein analysis Cetrimonium Compounds Detergents Equipment Design Octoxynol Proteins Sodium Dodecyl Sulfate Solutions Spectrometry, Mass, Electrospray Ionization
description	Detergents are necessarily used for different extraction protocols of proteins from biological cells or tissues. After the extraction, elimination of detergent is necessary for the better performance of electrospray ionization mass spectrometry (ESI-MS). Elimination of detergents is laborious and time-consuming, and also sample loss may be unavoidable. Probe electrospray ionization (PESI) developed in our laboratory has been found to be tolerant to the presence of salts and buffers in sample solutions. In this report, it was examined whether PESI is applicable to the sample solutions that contain high-concentration of detergents. It was found that PESI is highly tolerant to the presence of sodium dodecyl sulphate, cetyl trimethylamminium bromide, Triton X100 and 3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate compared with conventional ESI and nanoESI. Therefore, PESI can be a potential analytical tool for direct analysis of protein extracts and digests containing high-concentration detergents. Copyright © 2011 John Wiley & Sons, Ltd.
title	Detection of protein from detergent solutions by probe electrospray ionization mass spectrometry (PESI-MS)
title_short	Detection of protein from detergent solutions by probe electrospray ionization mass spectrometry (PESI-MS)
title_full	Detection of protein from detergent solutions by probe electrospray ionization mass spectrometry (PESI-MS)
title_fullStr	Detection of protein from detergent solutions by probe electrospray ionization mass spectrometry (PESI-MS)
title_full_unstemmed	Detection of protein from detergent solutions by probe electrospray ionization mass spectrometry (PESI-MS)
title_sort	detection of protein from detergent solutions by probe electrospray ionization mass spectrometry (pesi-ms)
publishDate	2011
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10765174_v46_n10_p967_Mandal http://hdl.handle.net/20.500.12110/paper_10765174_v46_n10_p967_Mandal
_version_	1768542562605334528

Detection of protein from detergent solutions by probe electrospray ionization mass spectrometry (PESI-MS)

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