Potato Virus X Coat Protein Fusion to Human Papillomavirus 16 E7 Oncoprotein Enhance Antigen Stability and Accumulation in Tobacco Chloroplast
Cervical cancer linked to infection with human papillomavirus (HPV) is the third cause of cancer-related death in women. As the virus cannot be propagated in culture, vaccines have been based on recombinant antigens with inherited high-cost production. In a search of alternative cheap production sys...
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2009
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10736085_v43_n3_p243_Morgenfeld http://hdl.handle.net/20.500.12110/paper_10736085_v43_n3_p243_Morgenfeld |
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paper:paper_10736085_v43_n3_p243_Morgenfeld2023-06-08T16:04:58Z Potato Virus X Coat Protein Fusion to Human Papillomavirus 16 E7 Oncoprotein Enhance Antigen Stability and Accumulation in Tobacco Chloroplast Morgenfeld, Mauro Miguel Segretin, María Eugenia Wirth, Sonia Alejandra Lentz, Ezequiel Matías Zelada, Alicia M. Mentaberry, Alejandro Néstor Chloroplast transformation E7 antigen Fusion protein Human papillomavirus (HPV) Molecular farming Transplastomic tobacco Chloroplast transformation E7 antigen Fusion protein Human papillomavirus (HPV) Molecular farming Transplastomic tobacco Amines Chlorophyll Peptides System stability Tobacco Antigens coat protein protein E7 article chloroplast gene sequence nonhuman nucleotide sequence polymerase chain reaction protein engineering protein expression protein stability Southern blotting stroma cell tobacco Blotting, Northern Blotting, Southern Capsid Proteins Chloroplasts Cloning, Molecular Oncogene Proteins, Viral Plants, Genetically Modified Polymerase Chain Reaction Protein Stability Recombinant Fusion Proteins Tobacco Human papillomavirus Human papillomavirus - 16 Human papillomavirus type 16 Nicotiana tabacum Potato virus X Cervical cancer linked to infection with human papillomavirus (HPV) is the third cause of cancer-related death in women. As the virus cannot be propagated in culture, vaccines have been based on recombinant antigens with inherited high-cost production. In a search of alternative cheap production system, E7 HPV type 16 protein, an attractive candidate for anticancer vaccine development, was engineered to be expressed in tobacco chloroplast. In addition, E7 coding sequence was fused to potato virus X coat protein (CP) to compare expression level. Results show that E7CP transcript accumulation reached lower levels than non-fused E7. However, antigen expression levels were higher for fusion protein indicating that CP stabilizes E7 peptide in the chloroplast stroma. These results support viability of transplastomic plants for antigen production and the relevance of improving recombinant peptide stability for certain transgenes to enhance protein accumulation in this organelle. © 2009 Humana Press. Fil:Morgenfeld, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Segretin, M.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Wirth, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Lentz, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Zelada, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Mentaberry, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2009 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10736085_v43_n3_p243_Morgenfeld http://hdl.handle.net/20.500.12110/paper_10736085_v43_n3_p243_Morgenfeld |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Chloroplast transformation E7 antigen Fusion protein Human papillomavirus (HPV) Molecular farming Transplastomic tobacco Chloroplast transformation E7 antigen Fusion protein Human papillomavirus (HPV) Molecular farming Transplastomic tobacco Amines Chlorophyll Peptides System stability Tobacco Antigens coat protein protein E7 article chloroplast gene sequence nonhuman nucleotide sequence polymerase chain reaction protein engineering protein expression protein stability Southern blotting stroma cell tobacco Blotting, Northern Blotting, Southern Capsid Proteins Chloroplasts Cloning, Molecular Oncogene Proteins, Viral Plants, Genetically Modified Polymerase Chain Reaction Protein Stability Recombinant Fusion Proteins Tobacco Human papillomavirus Human papillomavirus - 16 Human papillomavirus type 16 Nicotiana tabacum Potato virus X |
| spellingShingle |
Chloroplast transformation E7 antigen Fusion protein Human papillomavirus (HPV) Molecular farming Transplastomic tobacco Chloroplast transformation E7 antigen Fusion protein Human papillomavirus (HPV) Molecular farming Transplastomic tobacco Amines Chlorophyll Peptides System stability Tobacco Antigens coat protein protein E7 article chloroplast gene sequence nonhuman nucleotide sequence polymerase chain reaction protein engineering protein expression protein stability Southern blotting stroma cell tobacco Blotting, Northern Blotting, Southern Capsid Proteins Chloroplasts Cloning, Molecular Oncogene Proteins, Viral Plants, Genetically Modified Polymerase Chain Reaction Protein Stability Recombinant Fusion Proteins Tobacco Human papillomavirus Human papillomavirus - 16 Human papillomavirus type 16 Nicotiana tabacum Potato virus X Morgenfeld, Mauro Miguel Segretin, María Eugenia Wirth, Sonia Alejandra Lentz, Ezequiel Matías Zelada, Alicia M. Mentaberry, Alejandro Néstor Potato Virus X Coat Protein Fusion to Human Papillomavirus 16 E7 Oncoprotein Enhance Antigen Stability and Accumulation in Tobacco Chloroplast |
| topic_facet |
Chloroplast transformation E7 antigen Fusion protein Human papillomavirus (HPV) Molecular farming Transplastomic tobacco Chloroplast transformation E7 antigen Fusion protein Human papillomavirus (HPV) Molecular farming Transplastomic tobacco Amines Chlorophyll Peptides System stability Tobacco Antigens coat protein protein E7 article chloroplast gene sequence nonhuman nucleotide sequence polymerase chain reaction protein engineering protein expression protein stability Southern blotting stroma cell tobacco Blotting, Northern Blotting, Southern Capsid Proteins Chloroplasts Cloning, Molecular Oncogene Proteins, Viral Plants, Genetically Modified Polymerase Chain Reaction Protein Stability Recombinant Fusion Proteins Tobacco Human papillomavirus Human papillomavirus - 16 Human papillomavirus type 16 Nicotiana tabacum Potato virus X |
| description |
Cervical cancer linked to infection with human papillomavirus (HPV) is the third cause of cancer-related death in women. As the virus cannot be propagated in culture, vaccines have been based on recombinant antigens with inherited high-cost production. In a search of alternative cheap production system, E7 HPV type 16 protein, an attractive candidate for anticancer vaccine development, was engineered to be expressed in tobacco chloroplast. In addition, E7 coding sequence was fused to potato virus X coat protein (CP) to compare expression level. Results show that E7CP transcript accumulation reached lower levels than non-fused E7. However, antigen expression levels were higher for fusion protein indicating that CP stabilizes E7 peptide in the chloroplast stroma. These results support viability of transplastomic plants for antigen production and the relevance of improving recombinant peptide stability for certain transgenes to enhance protein accumulation in this organelle. © 2009 Humana Press. |
| author |
Morgenfeld, Mauro Miguel Segretin, María Eugenia Wirth, Sonia Alejandra Lentz, Ezequiel Matías Zelada, Alicia M. Mentaberry, Alejandro Néstor |
| author_facet |
Morgenfeld, Mauro Miguel Segretin, María Eugenia Wirth, Sonia Alejandra Lentz, Ezequiel Matías Zelada, Alicia M. Mentaberry, Alejandro Néstor |
| author_sort |
Morgenfeld, Mauro Miguel |
| title |
Potato Virus X Coat Protein Fusion to Human Papillomavirus 16 E7 Oncoprotein Enhance Antigen Stability and Accumulation in Tobacco Chloroplast |
| title_short |
Potato Virus X Coat Protein Fusion to Human Papillomavirus 16 E7 Oncoprotein Enhance Antigen Stability and Accumulation in Tobacco Chloroplast |
| title_full |
Potato Virus X Coat Protein Fusion to Human Papillomavirus 16 E7 Oncoprotein Enhance Antigen Stability and Accumulation in Tobacco Chloroplast |
| title_fullStr |
Potato Virus X Coat Protein Fusion to Human Papillomavirus 16 E7 Oncoprotein Enhance Antigen Stability and Accumulation in Tobacco Chloroplast |
| title_full_unstemmed |
Potato Virus X Coat Protein Fusion to Human Papillomavirus 16 E7 Oncoprotein Enhance Antigen Stability and Accumulation in Tobacco Chloroplast |
| title_sort |
potato virus x coat protein fusion to human papillomavirus 16 e7 oncoprotein enhance antigen stability and accumulation in tobacco chloroplast |
| publishDate |
2009 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10736085_v43_n3_p243_Morgenfeld http://hdl.handle.net/20.500.12110/paper_10736085_v43_n3_p243_Morgenfeld |
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| _version_ |
1768542232076353536 |