Inhibition of early endosome fusion by Trypanosoma cruzi-infected macrophage cytosol

Trypanosoma cruzi trypomastigotes survive inside macrophages by promoting fusion between the parasitophorous vacuole and mature host lysosomes upon internalization. Since trypomastigotes can evade the lyric pathway, the earliest steps of endocytosis, such as early endosome fusion, may be affected. T...

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Detalles Bibliográficos
Publicado:	1997
Materias:	Calcium supply GTPγS Trypomastigote-infected macrophage Animalia Trypanosoma Trypanosoma cruzi
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10665234_v44_n5_p497_Ochatt http://hdl.handle.net/20.500.12110/paper_10665234_v44_n5_p497_Ochatt
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_10665234_v44_n5_p497_Ochatt
record_format	dspace
spelling	paper:paper_10665234_v44_n5_p497_Ochatt2023-06-08T16:04:16Z Inhibition of early endosome fusion by Trypanosoma cruzi-infected macrophage cytosol Calcium supply GTPγS Trypomastigote-infected macrophage Animalia Trypanosoma Trypanosoma cruzi Trypanosoma cruzi trypomastigotes survive inside macrophages by promoting fusion between the parasitophorous vacuole and mature host lysosomes upon internalization. Since trypomastigotes can evade the lyric pathway, the earliest steps of endocytosis, such as early endosome fusion, may be affected. To test this hypothesis, we used an in vitro early endosome fusion assay. Our results show that trypomastigote-infected macrophage cytosols cannot promote fusion between early endosomes, compared to mock-infected cytosols (heat-killed trypomastigotes were used in the parasite-macrophage interaction assay). GTPγS addition potentiates the fusogenic activity driven by trypomastigote-infected macrophage cytosol-mediated assays, unlike the biphasic fusogenic effect obtained with GTPγS treatment of macrophage cytosol controls. Calcium-stimulated early endosome fusogenic processes are not affected in the assays mediated by infected macrophage cytosol. We conclude that GTP-regulated factors, and not calcium-regulated elements, are involved in the inhibition of the early endosome fusogenic process by the trypomastigote-infected macrophage cytosol. This primary impediment to the progress of a normal endocytosis may be a relevant step required for the lysosomal recruitment-fusion of the host lysosomes upon trypomastigote infection and further survival of the parasite within its host. 1997 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10665234_v44_n5_p497_Ochatt http://hdl.handle.net/20.500.12110/paper_10665234_v44_n5_p497_Ochatt
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Calcium supply GTPγS Trypomastigote-infected macrophage Animalia Trypanosoma Trypanosoma cruzi
spellingShingle	Calcium supply GTPγS Trypomastigote-infected macrophage Animalia Trypanosoma Trypanosoma cruzi Inhibition of early endosome fusion by Trypanosoma cruzi-infected macrophage cytosol
topic_facet	Calcium supply GTPγS Trypomastigote-infected macrophage Animalia Trypanosoma Trypanosoma cruzi
description	Trypanosoma cruzi trypomastigotes survive inside macrophages by promoting fusion between the parasitophorous vacuole and mature host lysosomes upon internalization. Since trypomastigotes can evade the lyric pathway, the earliest steps of endocytosis, such as early endosome fusion, may be affected. To test this hypothesis, we used an in vitro early endosome fusion assay. Our results show that trypomastigote-infected macrophage cytosols cannot promote fusion between early endosomes, compared to mock-infected cytosols (heat-killed trypomastigotes were used in the parasite-macrophage interaction assay). GTPγS addition potentiates the fusogenic activity driven by trypomastigote-infected macrophage cytosol-mediated assays, unlike the biphasic fusogenic effect obtained with GTPγS treatment of macrophage cytosol controls. Calcium-stimulated early endosome fusogenic processes are not affected in the assays mediated by infected macrophage cytosol. We conclude that GTP-regulated factors, and not calcium-regulated elements, are involved in the inhibition of the early endosome fusogenic process by the trypomastigote-infected macrophage cytosol. This primary impediment to the progress of a normal endocytosis may be a relevant step required for the lysosomal recruitment-fusion of the host lysosomes upon trypomastigote infection and further survival of the parasite within its host.
title	Inhibition of early endosome fusion by Trypanosoma cruzi-infected macrophage cytosol
title_short	Inhibition of early endosome fusion by Trypanosoma cruzi-infected macrophage cytosol
title_full	Inhibition of early endosome fusion by Trypanosoma cruzi-infected macrophage cytosol
title_fullStr	Inhibition of early endosome fusion by Trypanosoma cruzi-infected macrophage cytosol
title_full_unstemmed	Inhibition of early endosome fusion by Trypanosoma cruzi-infected macrophage cytosol
title_sort	inhibition of early endosome fusion by trypanosoma cruzi-infected macrophage cytosol
publishDate	1997
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10665234_v44_n5_p497_Ochatt http://hdl.handle.net/20.500.12110/paper_10665234_v44_n5_p497_Ochatt
_version_	1768544970446209024

Inhibition of early endosome fusion by Trypanosoma cruzi-infected macrophage cytosol

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