Altered expression of Bcl-2 and Bax in follicles within dehydroepiandrosterone-induced polycystic ovaries in rats
PCOS (polycystic ovary syndrome) is a heterogeneous disease characterized by hyperandrogenaemia, hirsutism, oligo- or amenorrhea, insulin resistance and anovulation. The aim of the present study was to evaluate if the balance between the ovarian expression of Bax (proapoptotic protein) and Bcl-2 (an...
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2011
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paper:paper_10656995_v35_n5_p423_Bas2023-06-08T16:04:15Z Altered expression of Bcl-2 and Bax in follicles within dehydroepiandrosterone-induced polycystic ovaries in rats Abramovich, Dalhia Nurit Tesone, Marta Apoptotic protein Dehydroepiandrosterone Polycystic ovary syndrome prasterone progesterone protein Bax protein bcl 2 animal cell animal experiment animal model animal tissue apoptosis article cell structure cellular distribution controlled study corpus luteum female follicle cyst histopathology immunohistochemistry nonhuman ovary follicle cell ovary polycystic disease pathogenesis protein analysis protein expression protein function protein localization rat Western blotting Animals bcl-2-Associated X Protein Dehydroepiandrosterone Female Gene Expression Regulation Ovary Polycystic Ovary Syndrome Progesterone Proto-Oncogene Proteins c-bcl-2 Rats Rats, Sprague-Dawley Rattus PCOS (polycystic ovary syndrome) is a heterogeneous disease characterized by hyperandrogenaemia, hirsutism, oligo- or amenorrhea, insulin resistance and anovulation. The aim of the present study was to evaluate if the balance between the ovarian expression of Bax (proapoptotic protein) and Bcl-2 (antiapoptotic protein) is altered in a PCOS model developed in rats by DHEA (dehydroepiandrosterone) administration. In addition, the ovarian morphology and the circulating progesterone levels were evaluated. Histological studies confirmed the presence of follicular cysts, atretic follicles and the absence of corpora lutea in the ovaries from the PCOS group and a significant decrease in circulating progesterone levels. Immunohistochemical studies showed that the expression of Bcl-2 and Bax were mainly localized in granulosa cells of AFs (antral follicles) in both groups. Bax expression was greater in preantral and AFs from PCOS ovarian sections than in the controls. In contrast, intense Bcl-2 immunostaining was observed in the control AFs, while Bcl-2 protein was either absent in PFs (preantral follicles) or weakly expressed in AFs from PCOS rats. These results were partially confirmed by Western studies. Data revealed that the ovarian level of Bcl-2 protein was lower in PCOS than in the control and that there were no differences in Bax ovarian levels between groups. However, Bax/Bcl-2 ratio was significantly higher in PCOS group than in the control group. In conclusion, an increase in ovarian apoptosis through an imbalance among the Bcl-2 family members may be involved in the transformation of growing follicles in cystic follicles in the ovaries from DHEA-induced PCOS rats. © The Author(s) Journal compilation © 2011 Portland Press Limited. Fil:Abramovich, D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Tesone, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2011 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10656995_v35_n5_p423_Bas http://hdl.handle.net/20.500.12110/paper_10656995_v35_n5_p423_Bas |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Apoptotic protein Dehydroepiandrosterone Polycystic ovary syndrome prasterone progesterone protein Bax protein bcl 2 animal cell animal experiment animal model animal tissue apoptosis article cell structure cellular distribution controlled study corpus luteum female follicle cyst histopathology immunohistochemistry nonhuman ovary follicle cell ovary polycystic disease pathogenesis protein analysis protein expression protein function protein localization rat Western blotting Animals bcl-2-Associated X Protein Dehydroepiandrosterone Female Gene Expression Regulation Ovary Polycystic Ovary Syndrome Progesterone Proto-Oncogene Proteins c-bcl-2 Rats Rats, Sprague-Dawley Rattus |
| spellingShingle |
Apoptotic protein Dehydroepiandrosterone Polycystic ovary syndrome prasterone progesterone protein Bax protein bcl 2 animal cell animal experiment animal model animal tissue apoptosis article cell structure cellular distribution controlled study corpus luteum female follicle cyst histopathology immunohistochemistry nonhuman ovary follicle cell ovary polycystic disease pathogenesis protein analysis protein expression protein function protein localization rat Western blotting Animals bcl-2-Associated X Protein Dehydroepiandrosterone Female Gene Expression Regulation Ovary Polycystic Ovary Syndrome Progesterone Proto-Oncogene Proteins c-bcl-2 Rats Rats, Sprague-Dawley Rattus Abramovich, Dalhia Nurit Tesone, Marta Altered expression of Bcl-2 and Bax in follicles within dehydroepiandrosterone-induced polycystic ovaries in rats |
| topic_facet |
Apoptotic protein Dehydroepiandrosterone Polycystic ovary syndrome prasterone progesterone protein Bax protein bcl 2 animal cell animal experiment animal model animal tissue apoptosis article cell structure cellular distribution controlled study corpus luteum female follicle cyst histopathology immunohistochemistry nonhuman ovary follicle cell ovary polycystic disease pathogenesis protein analysis protein expression protein function protein localization rat Western blotting Animals bcl-2-Associated X Protein Dehydroepiandrosterone Female Gene Expression Regulation Ovary Polycystic Ovary Syndrome Progesterone Proto-Oncogene Proteins c-bcl-2 Rats Rats, Sprague-Dawley Rattus |
| description |
PCOS (polycystic ovary syndrome) is a heterogeneous disease characterized by hyperandrogenaemia, hirsutism, oligo- or amenorrhea, insulin resistance and anovulation. The aim of the present study was to evaluate if the balance between the ovarian expression of Bax (proapoptotic protein) and Bcl-2 (antiapoptotic protein) is altered in a PCOS model developed in rats by DHEA (dehydroepiandrosterone) administration. In addition, the ovarian morphology and the circulating progesterone levels were evaluated. Histological studies confirmed the presence of follicular cysts, atretic follicles and the absence of corpora lutea in the ovaries from the PCOS group and a significant decrease in circulating progesterone levels. Immunohistochemical studies showed that the expression of Bcl-2 and Bax were mainly localized in granulosa cells of AFs (antral follicles) in both groups. Bax expression was greater in preantral and AFs from PCOS ovarian sections than in the controls. In contrast, intense Bcl-2 immunostaining was observed in the control AFs, while Bcl-2 protein was either absent in PFs (preantral follicles) or weakly expressed in AFs from PCOS rats. These results were partially confirmed by Western studies. Data revealed that the ovarian level of Bcl-2 protein was lower in PCOS than in the control and that there were no differences in Bax ovarian levels between groups. However, Bax/Bcl-2 ratio was significantly higher in PCOS group than in the control group. In conclusion, an increase in ovarian apoptosis through an imbalance among the Bcl-2 family members may be involved in the transformation of growing follicles in cystic follicles in the ovaries from DHEA-induced PCOS rats. © The Author(s) Journal compilation © 2011 Portland Press Limited. |
| author |
Abramovich, Dalhia Nurit Tesone, Marta |
| author_facet |
Abramovich, Dalhia Nurit Tesone, Marta |
| author_sort |
Abramovich, Dalhia Nurit |
| title |
Altered expression of Bcl-2 and Bax in follicles within dehydroepiandrosterone-induced polycystic ovaries in rats |
| title_short |
Altered expression of Bcl-2 and Bax in follicles within dehydroepiandrosterone-induced polycystic ovaries in rats |
| title_full |
Altered expression of Bcl-2 and Bax in follicles within dehydroepiandrosterone-induced polycystic ovaries in rats |
| title_fullStr |
Altered expression of Bcl-2 and Bax in follicles within dehydroepiandrosterone-induced polycystic ovaries in rats |
| title_full_unstemmed |
Altered expression of Bcl-2 and Bax in follicles within dehydroepiandrosterone-induced polycystic ovaries in rats |
| title_sort |
altered expression of bcl-2 and bax in follicles within dehydroepiandrosterone-induced polycystic ovaries in rats |
| publishDate |
2011 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10656995_v35_n5_p423_Bas http://hdl.handle.net/20.500.12110/paper_10656995_v35_n5_p423_Bas |
| work_keys_str_mv |
AT abramovichdalhianurit alteredexpressionofbcl2andbaxinfollicleswithindehydroepiandrosteroneinducedpolycysticovariesinrats AT tesonemarta alteredexpressionofbcl2andbaxinfollicleswithindehydroepiandrosteroneinducedpolycysticovariesinrats |
| _version_ |
1768542851522625536 |